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  • 1
    Electronic Resource
    Electronic Resource
    Spinocerebellar ataxia type 5 in a family descended from the grandparents of President Lincoln maps to chromosome 11 (1994)
    [s.l.] : Nature Publishing Group
    Nature genetics 8 (1994), S. 280-284 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Autosomal dominant ataxias are a genetically heterogeneous group of disorders for which spinocerebellar ataxia (SCA) loci on chromosomes 6p, 12q, 14q and 16q have been reported. We have examined 170 individuals (56 of whom were affected) from a previously unreported ten-generation kindred with a ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/ng1194-280
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  • 2
    Electronic Resource
    Electronic Resource
    The effect of canavanine on the maintenance in yeast of chimeric plasmids containing portions of the 2-µm DNA plasmid (1983)
    Springer
    Current genetics 7 (1983), S. 363-367 
    Details
    ISSN: 1432-0983
    Keywords: Canavanine ; Yeast ; Plasmids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have found that the application of the amino acid analog canavanine to a culture of yeast cells transformed with chimeric plasmids based on the yeast 2-µm DNA plasmid increases the percentage of cells which have lost the transforming plasmid. This effect is found whether the plasmid carries the CAN1 sensitive allele and the yeast strain carries a can1 mutation confering resistance, or the plasmid contains no CAN1 allele and the yeast strain carries the wild-type CAN1 sensitive allele. Canavanine exerts this effect on yeast strains transformed with chimeric plasmids containing either a portion or the entire 2-µm DNA plasmid, yet canavanine does not appear to effect the maintenance of the native 2-µm DNA plasmid complement within the cell. The effect of canavanine on strains transformed with chimeric plasmids is the same whether or not the yeast strain also contains native 2-µm plasmid DNA. Neither the amino acid analog ethionine, the protein synthesis inhibitor cycloheximide, nor the DNA replication inhibitor hydroxyurea exhibit this effect. Some of the experimental results suggest that canavanine may be a curing agent rather than an agent which selects for spontaneous plasmid loss.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00445876
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Allele-specific suppression of temperature-sensitive mutations of theSaccharomyces cerevisiae RAD52 gene (1996)
    Springer
    Current genetics 29 (1996), S. 203-210 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Mutations ; Suppressors ; RAD52
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We screened forrad52 suppressors against temperature-sensitive (ts), missense, nonsense, and deletionrad52 mutations. Except for the deletion strain all mutants yielded suppressor candidates, indicating that suppressors completely bypassing the need forRAD52 are rare. Characterization of seven, recessive extragenic suppressors from our screen and two previously identified suppressors revealed that nearly all exhibit allele specificity. The allele specificity is positional in that suppressors that suppress a is mutation in the C-terminal third of the coding region do not suppress three is mutations in the N-terminal third. Conversly, suppressors against one of the three N-terminal mutations suppress more than one of these mutations but not the C-terminal mutation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02221549
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  • 4
    Electronic Resource
    Electronic Resource
    Characterization of the transmission during cytoductant formation of the 2 μm DNA plasmid from Saccharomyces (1981)
    Springer
    Molecular genetics and genomics 183 (1981), S. 59-65 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The transmission of the yeast 2 μm DNA plasmid has been examined in heterokaryons formed between a haploid donor cell containing the plasmid and a haploid recipient cell lacking the plasmid. Strains lacking the plasmid were mated to donor strains and cytoductants, haploid exconjugants arising from heterokaryons, were selected. The cytoductants bearing the genotype of the recipient cells were then tested for the presence of 2 μm DNA. The frequency with which the recipient received plasmid copies varied between 0 and 46%. This frequency depended on the recipient strain but was not highly dependent on whether or not the donor strain carried a kar1 mutation. Exceptional cytoductants, which had acquired a chromosome from the mating partner, were examined and found to have a much greater probability of acquiring plasmid DNA than cytoductants in general. This correlation supports the contention that plasmid copies are associated with the nucleus. In one mating the donor strain contained nearly equal amounts of two physically distinct plasmid types. Of the cytoductants bearing the recipient genotype which had acquired the plasmid during mating, most contained only one of the two plasmid types present in the donor. Analysis of this result using a Poisson distribution indicates that the average number of plasmid copies transmitted between nuclei of a heterokaryon is 0.2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270139
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  • 5
    Electronic Resource
    Electronic Resource
    Accumulation of single strand interruptions within the yeast 2 μm DNA plasmid during replication in a DNA ligase mutant (1986)
    Springer
    Molecular genetics and genomics 203 (1986), S. 406-409 
    Details
    ISSN: 1617-4623
    Keywords: Yeast ; 2μm plasmid ; Ligase mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have investigated the fate of the yeast 2 μm DNA plasmid in strains with a temperature sensitive mutation of DNA ligase. At the restrictive temperature the plasmid DNA collects as an open circular form with single strand interruptions. Both alpha factor pheromone, which arrests cells before the start of S phase, and hydroxyurea, which blocks progression through S phase, prevent the appearance of the open circular form. Thus, interrupted plasmid DNA does not accumulate in the absence of DNA replication. On average the interrupted molecules contain four to five interruptions per newly replicated strand. Most of the interruptions are nicks (breaks in a single phosphate ester bond) rather than gaps (absence of one or more nucleotides in a strand) as judged by the in vitro conversion of the interrupted molecules into a covalently closed form by DNA ligase. Mapping of the position of the interruptions reveals no predominate sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422064
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  • 6
    Electronic Resource
    Electronic Resource
    Cell cycle inhibition of yeast spheroplasts (1982)
    Springer
    Molecular genetics and genomics 185 (1982), S. 506-509 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Osmotically stabilized yeast spheroplasts are capable of extensive DNA synthesis. Although the rate of DNA synthesis in spheroplasts is approximately one-third that of intact cells, the relative amounts of nuclear and mitochondrial DNA synthesized by spheroplasts is very similar to the relative amounts synthesized by intact cells. Furthermore, nuclear but not mitochondrial DNA synthesis is inhibited in MATa spheroplasts by the application of the yeast mating pheromone, α-factor. Similarly, DNA synthesis is reversibly temperature-sensitive in spheroplasts created from cdc7 and cdc8 mutant cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334149
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  • 7
    Electronic Resource
    Electronic Resource
    GSG1, a yeast gene required for sporulation (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 11 (1995), S. 1147-1155 
    Details
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; sporulation ; meiosis ; GSG1 ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have identified a gene, GSG1 (general sporulation gene 1), required for sporulation in Saccharomyces cerevisiae. Diploids homozygous for a disruption of GSG1 fail to sporulate. The gene has an open reading frame of 2094 bp, encoding a polypeptide with an expected size of 77 kDa. GSG1 is expressed mitotically in both a and α haploids, and both mitotically and meiotically in diploids. The message level of GSG1 increases approximately two-fold after 4-6 h of sporulation. gsg1 mutants enter pre-meiotic DNA synthesis later than wild-type diploids. Mutant diploids are not rescued by spo13. These results suggest that GSG1 has a role late in meiosis following DNA replication. The sequence reported here has the GenBank Accession Number U26674.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320111205
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    The Effect of a Suppressed rad52 Mutation on the Suppression of rad6 by srs2 (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 13 (1997), S. 1059-1064 
    Details
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; DNA repair ; UV damage ; RAD52 ; proteosome ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have cloned a suppressor of a temperature-sensitive rad52 allele and found it to be a mutation in PUP1, a gene encoding a protease subunit of the 20s proteasome. This identity prompted us to examine the interrelationship among PUP1, RAD52, SRS2 and RAD6 because srs2 mutations not only suppress some rad52 mutations but also suppress deletions of rad6, a gene encoding a protein in the ubiquination-dependent proteolysis pathway. We have found that while srs2 suppresses the UV sensitivity of rad6 in the presence of RAD52, srs2 cannot suppress rad6 when the temperature-sensitive allele of rad52 is present. This inability of srs2 to suppress rad6 is irrespective of the incubation temperature or whether pup1 is suppressing the temperature-sensitive rad52 mutation. © 1997 by John Wiley & Sons, Ltd.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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