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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 28 (1980), S. 666-667 
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 110 (1993), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: All wheat varieties (106) grown in the U.S. on more than 100,000 acres (38,610 ha) as of the latest (1984) crop variety survey were characterized by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The high-molecular-weight glutenin subunit (HMW-GS) band patterns for each variety were assigned the corresponding Payne numbers and theoretical quality scores based on those assignments. The subunit assignments were compared for the different wheat varieties and the five main wheat classes grown in the U.S. Hard red spring (HRS) and winter (HRW) wheats used mainly for breadmaking showed a remarkably high percentage of bands associated with good breadmaking quality. The allele 5+10, which has the strongest association with good quality, was present in 91 % of the hard red spring wheats and 62 % of the hard red winter wheats. Also, 91 % of all HRS and 53 % of HRW wheat varieties had quality scores of 9 or 10 (10 is the highest possible score). Evidently, by selecting for quality through close cooperation with quality testing laboratories, U.S. breeders have unknowingly selected for high quality glutenin subunits in their released varieties.HRS and HRW wheat varieties are normally grown in different environments in time and/or space, accounting to a large extent for differences in protein content (∼2 %) and other quality traits in the two crops. The uniformly high theoretical quality scores of the HRS wheats compared to more variable scores for HRW wheats may help to explain the popular perception that spring wheats have intrinsically higher quality than winter wheats. Admixing grain from variable (some poorer, most good) HRW wheat varieties (due to genetics or environment) has probably also led to the perception of overall lower quality for HRW than HRS wheats.In the soft red winter (SRW) and soft white (SW) wheat classes where the end-use is typically cookies and cakes, 40 and 90 %, respectively, have the allele 2+12 that correlates with poor bread baking quality. The absence of alleles for good bread baking quality may be predictive of good quality for soft wheat products.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Tissue culture ; Electrophoresis ; Storage proteins ; Mutation ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fertile r0 plants of the winter wheat line ND7532 (Triticum aestivum L.) were regenerated from callus tissue after 60–190 days in culture. Seeds produced from these self-pollinated plants were planted in the field. Of the 5586 R1 plants, 32 differed for one or more agronomic traits from plants not passed through tissue culture process. Gliadin electrophoregrams were prepared from bulk samples of R2 seed from these 32 plants. Four of the 32 produced gliadin patterns different from controls, so 12 seeds of each of these four lines were examined individually. Three of the four mutant lines were fixed for the presence of a mutant protein of 50 relative mobility units (RMU) and the corresponding loss of a parental protein of 26 RMU. The remaining line segregated for the presence/absence of band 50 and the corresponding loss/retention of band 26. The mutant protein of 50 RMU was never seen in control plants. This indicated that either band 50 was coded for by a mutant gene allelic to the gene that coded for band 26 or that bands 26 and 50 were coded for by two different structural alleles under the control of a common regulatory locus. Each of the 12 seeds from the four mutant lines contained a prominent protein band at 30 (RMU), which was only observed as a faint band in one control seed. The types of variation in gliadin patterns observed in somaclones of ND7532 were similar to those reported for the line ‘Yaqui 50E’, except that, gliadin changes occurred less frequently in ND7532.
    Type of Medium: Electronic Resource
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