ISSN:
1432-072X
Keywords:
Key words DMSO respiration
;
DMS production
;
Anaerobic respiration
;
Anaerobic regulation
;
Wolinella succinogens
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The anaerobic rumen bacterium Wolinella succinogenes was able to grow by respiration with dimethylsulphoxide (DMSO) as electron acceptor and formate or H2 as electron donors. The growth yield amounted to 6.7 g and 6.4 g dry cells/mol DMSO with formate or H2 as the donors, respectively. This suggested an ATP yield of about 0.7 mol ATP/mol DMSO. Cell homogenates and the membrane fraction con tained DMSO reductase activity with a high K m (43 mM) for DMSO. The electron transport from H2 to DMSO in the membranes was inhibited by 2-(heptyl)-4-hydroxyquinoline N-oxide, indicating the participation of menaquinone. Formation of DMSO reductase activity occurred only during growth on DMSO, presence of other electron acceptors (fumarate, nitrate, nitrite, N2O, and sulphur) repressed the DMSO reductase activity. DMSO can therefore be used by W. succin ogenes as an acceptor for phosphorylative electron transport, but other electron acceptors are used preferentially.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00301851
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