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  • 1
    Electronic Resource
    Electronic Resource
    Intergeneric somatic hybridization of sexually incompatible parents: Citrus sinensis and Atalantia ceylanica (1993)
    Springer
    Plant cell reports 12 (1993), S. 687-690 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protoplast fusion using polyethylene glycol (PEG) was conducted to combine Citrus sinensis (L.) Osbeck cv. ‘Hamlin’ sweet orange protoplasts, isolated from nucellus-derived embryogenic callus with Atalantia ceylanica (Arn.) Oliv, leaf protoplasts. Five plants regenerated from independent fusion events following protoplast culture were identified as intergeneric allotetraploid somatic hybrids of ‘Hamlin’ sweet orange and A. ceylanica, and confirmed by isozyme analysis and chromosome number determination in root tip cells (2n=4x=36). Two different types of leaf morphology were observed among the hybrids (normal and narrow), although no differences in chromosome number nor isozyme banding patterns were observed. This is the first report of the production of hybrid plants between these sexually incompatible genera.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233420
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Allotetraploid hybrids between citrus and seven related genera produced by somatic hybridization (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 577-582 
    Details
    ISSN: 1432-2242
    Keywords: Key words  Citrus cultivar improvement ; Germ plasm expansion ; Protoplast fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   We have developed an efficient protoplast-fusion method to produce somatic hybrid allopolyploid plants that combine Citrus with seven related genera, including four that are sexually incompatible. In this paper we report the creation of 18 new allotetraploid hybrids of Citrus, including ten among sexually incompatible related genera, that may have direct cultivar potential as improved citrus rootstocks. All hybrids were confirmed by cytological and RAPD analyses. If fertile, the attributes of these hybrids may be amenable to further genetic manipulation by breeding at the tetraploid level. Wide somatic hybridization of Citrus via protoplast fusion bypasses biological barriers to the natural allopolyploidization of Citrus, and creates new evolutionary opportunities that would be difficult or impossible to achieve by natural or conventional hybridization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050166
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Allotetraploid hybrids between citrus and seven related genera produced by somatic hybridization (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 577-582 
    Details
    ISSN: 1432-2242
    Keywords: Citrus cultivar improvement ; Germ plasm expansion ; Protoplast fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have developed an efficient protoplast-fusion method to produce somatic hybrid allopolyploid plants that combine Citrus with seven related genera, including four that are sexually incompatible. In this paper we report the creation of 18 new allotetraploid hybrids of Citrus, including ten among sexually incompatible related genera, that may have direct cultivar potential as improved citrus rootstocks. All hybrids were confirmed by cytological and RAPD analyses. If fertile, the attributes of these hybrids may be amenable to further genetic manipulation by breeding at the tetraploid level. Wide somatic hybridization of Citrus via protoplast fusion bypasses biological barriers to the natural allopolyploidization of Citrus, and creates new evolutionary opportunities that would be difficult or impossible to achieve by natural or conventional hybridization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224561
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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