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  • 1
    Electronic Resource
    Electronic Resource
    Molecular cloning of a cDNA fromBrassica napus L. for a homologue of acyl-CoA-binding protein (1994)
    Springer
    Plant molecular biology 25 (1994), S. 917-920 
    Details
    ISSN: 1573-5028
    Keywords: acyl-CoA-binding protein ; Brassica napus ; diazepam-binding inhibitor protein ; linkage map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA encoding an acyl-CoA-binding protein (ACBP) homologue has been cloned from a λgt11 library made from mRNA isolated from developing seeds of oilseed rape (Brassica napus L.). The derived amino acid sequence reveals a protein 92 amino acids in length which is highly conserved when compared with ACBP sequences from yeast, cow, man and fruit fly. Southern blot analysis ofBrassica napus genomic DNA revealed the presence of 6 genes, 3 derived from theBrassica rapa parent and 3 fromBrassica oleracea. Northern blot analysis showed that ACBP genes are expressed strongly in developing embryo, flowers and cotyledons of seedlings and to a lesser extent in leaves and roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028886
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  • 2
    Electronic Resource
    Electronic Resource
    A 2.6 kb DNA sequence of Streptomyces coelicolor A3(2) which functions as a transposable element (1986)
    Springer
    Molecular genetics and genomics 203 (1986), S. 79-88 
    Details
    ISSN: 1617-4623
    Keywords: Streptomyces ; Transposon ; Plasmid ; Integration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Streptomyces coelicolor A3(2) contains CCC DNA molecules, 2.6 kb in size, with an average copy number of less than one per ten chromosomes. Southern hybridisation revealed, in addition, two linear, integrated copies (A and B) of this “mini-circle” sequence per chromosome. The two integrated copies have similar (if not identical) ends and are present in the same locations in various S. coelicolor A3(2) derivatives. The mini-circle sequence is absent from S. lividans 66 and S. violaceolatus ISP5438 and from several Streptomyces species less closely related to S. coelicolor A3(2). None of a variety of Streptomyces plasmids tested contained homology to the mini-circle sequence. When a 1.8 kb fragment of the mini-circle lacking the ends of the integrated copies was inserted into KC515 (a derivative of the temperate phage ϕC31 which is unable to lysogenise host strains by the natural route because the phage attachment site has been deleted) the resulting phage lysogenised S. coelicolor A3(2) (integrating into the genome of this host by homologous recombination with resident minicircle sequences) but not S. lividans or a variety of other ϕC31 hosts. In contrast, a KC515 derivative (KC591) carrying the entire 2.6 kb mini-circle sequence linearised at its single BclI site (and therefore containing the integration site of the free mini-circle) lysogenised not only S. coelicolor A3(2) but also S. lividans 66 and most other strains normally lysogenised by ϕC31. The KC591 lysogens of the eight Streptomyces species tested contained a linear, integrated prophage with termini apparently identical to those of the linear mini-cricle copies of S. coelicolor. In S. lividans, KC591 integrated preferentially at a site apparently homologous to the site occupied by mini-circle sequence A in S. coelicolor A3(2) strains, but integration into secondary sites also occurred.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330387
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  • 3
    Electronic Resource
    Electronic Resource
    Mutation and cloning of clustered Streptomyces genes essential for sulphate metabolism (1988)
    Springer
    Molecular genetics and genomics 211 (1988), S. 415-423 
    Details
    ISSN: 1617-4623
    Keywords: Streptomyces ; Selenate resistance ; Cysteine auxotrophy ; Sulphate permease ; ATP sulphurylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A range of mutants auxotrophic for cysteine (cys) and resistant to selenate (sel) were isolated from many Streptomyces strains but chiefly from S. coelicolor A3(2) and S. lividans 66. Two of the classes of sel/cys mutants probably contained simple biochemical lesions of sulphate permease (selC) and ATP sulphurylase (selA) activities, while a further two classes (selD and selE) were pleiotropic and possibly regulatory. Most classes of sel mutations were clustered around the cysD locus of S. coelicolor. Segments of chromosomal DNA cloned from S. coelicolor, S. cattleya and S. clavuligerus and able to complement various sel/cys mutations allowed the relative positions of these mutations and the cysC and cysD mutations of S. coelicolor to be determined. The sel/cys DNA can be used for two-way selection: Cys+Sels↔Cys-Selr.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425694
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  • 4
    Electronic Resource
    Electronic Resource
    Selecting the optimum genetic background for transgenic varieties, with examples from Brassica (1955)
    Springer
    Euphytica 85 (1955), S. 351-358 
    Details
    ISSN: 1573-5060
    Keywords: binomial distribution ; genetic complexity ; interspecific crosses ; introgression ; marker-assited selection ; modifier genes ; recombination frequency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The performance of transgenic varieties depends not only upon the stable and correctly-regulated expression of specific transgenes but also upon the agronomic potential of the background genotype. Ideally, transgenes should be introduced into agronomically-superior cultivars and transgenic varieties will become out-classed if their agronomic properties are not continually improved. It will often prove desirable to use conventional breeding techniques, as opposed to new cycles of transformation, to carry out this process of varietal improvement. Continuing advances in marker-assisted selection have made possible the selection and manipulation of an entire genetic background. This means that transgenes can be transferred to new and often ‘untransformable’ varieties with relative ease. To carry out this process efficiently requires the correct choice of male and female parents, the use of appropriate marker-systems and the concentration of selection on the most appropriate generations. Efficient, phenotypically-neutral marker-systems have revolutionised the identification and manipulation of quantitative trait loci (QTLs). The loci which modify the expression of transgenes are a form of QTL. Desirable alleles at modifier QTLs can be transferred to new varieties along with the transgenes themselves, using marker-assisted breeding. The strategies for maker-assisted selection are becoming ever more sophisticated. A range of complementary marker systems allows the selection of desirable genotypes. In addition, the meiotic reassortment and recombination of chromosomes which produces new genotypes is becoming better understood. The most efficient plant breeding methods and the most powerful genetics will make optimal use of both markers and meiosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023967
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Conserved structure and function of the Arabidopsis flowering time gene CONSTANS in Brassica napus (1998)
    Springer
    Plant molecular biology 37 (1998), S. 763-772 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; Brassica napus ; constans ; flowering ; zinc finger
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Arabidopsis thaliana CONSTANS (CO) gene which promotes flowering in long days was recently isolated by chromosome walking. The mapping of QTLs controlling flowering time in Brassica species has identified genomic regions that contain homologues of the CO gene. Four genes homologous to the Arabidopsis CO gene were isolated from a pair of homoeologous loci in each of two doubled-haploid Brassica napus lines displaying different flowering times, N-o-1 and N-o-9. The four genes, BnCOa1, BnCOa9, BnCOb1 and BnCOb9, are located on linkage groups N10 and N19, and are highly similar to each other and to the Arabidopsis CO gene. Two regions of the proteins are particularly well conserved, a N-terminal region with two putative zinc fingers and a C-terminal region which may contain a nuclear localization signal. All four genes appear to be expressed in B. napus. The BnCOa1 allele was shown to complement the co-2 mutation in Arabidopsis in a dosage-dependent manner causing earlier flowering than in wild type under both long- and short-day conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006064514311
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