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  • 1
    Electronic Resource
    Electronic Resource
    Chemotactic and random movement of cord-blood granulocytes (1984)
    Springer
    Cellular and molecular life sciences 40 (1984), S. 1407-1410 
    Details
    ISSN: 1420-9071
    Keywords: Granulocytes, cord-blood ; random movement ; chemotactic responsiveness ; chemotaxis inhibitors ; cyclic nucleotide levels
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Chemotactic responsiveness and random movement of cord-blood granulocytes were studied with a modified Boyden's method. Cord-blood granulocytes were less active chemotactically than granulocytes from healthy children and adults, whereas the random filter movement of the cells from all three sources was about the same. In cord sera, concentrations of cell directed chemotaxis inhibitors were equal to those in sera from other age groups. Compared with the situation in healthy children and adults, the generation of chemotactic factors in cord-blood sera was impaired. This impairment was not related to an increased activity of chemotactic factor inactivators. Measurement of the cyclic nucleotide levels in granulocytes from cord-blood and from children belonging to various age groups revealed that the cord granulocytes have significantly lower concentrations of cAMP and cGMP, which could have been responsible for the decreased chemotactic responsiveness.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01951915
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Chemotactic and random movement of human newborn monocytes (1980)
    Springer
    European journal of pediatrics 135 (1980), S. 73-75 
    Details
    ISSN: 1432-1076
    Keywords: Monocyte chemotaxis ; Random movement ; Boyden chamber ; Lower surface count method ; Leading front method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Boyden's method was used to assess the chemotactic and random mobility of monocytes from healthy newborns, children aged 3–8 years, and adults aged 20–28. Monocyte chemotaxis in the newborn was decreased in comparison with that of control groups. No difference was observed in random cell mobility in the groups examined. Monocyte chemotactic activity in infants over 3 years of age was the same as in adults. According to our results, the leading-front and lower-surface evaluation methods, in newborns at least, do not give the same information on chemotaxis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00445897
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Immunoglobulin deficiency with increased immunoglobulin M in three siblings: effect of long-term immunoglobulin therapy (1988)
    Springer
    European journal of pediatrics 148 (1988), S. 215-217 
    Details
    ISSN: 1432-1076
    Keywords: Immunodeficiency ; Hyper-IgM syndrome ; Siblings ; Immunoglobulin treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Diagnosis of immunoglobulin deficiency with increased IgM (hyper-IgM syndrome) was made in three siblings (two girls and a boy) on the basis of history, physical findings, and laboratory data. The prominent clinical findings were recurrent viral and bacterial infections of the respiratory tract. The most severe infections affected the male patient, who died at the age of 8 years. Family history and the lack of clinical signs in the parents and relatives indicated no immunodeficiency which, together with the occurrence of the disease in both sexes, indicated an autosomal recessive inheritance. The two female patients (18 years old and 3 years old) have been treated with intravenous acid-treated immunoglobulin for 2 years, resulting in significant clinical improvement with respect to the frequency and severity of infections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00441406
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Flow cytometric assay of phagocytic activity of human neutrophils and monocytes in whole blood by neutral red uptake (1995)
    Springer
    Annals of hematology 70 (1995), S. 259-265 
    Details
    ISSN: 1432-0584
    Keywords: Phagocytosis ; Neutrophils ; Monocytes ; Flow cytometry ; Neutral red
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In a new, simple, and fast flow-cytometric method for the simultaneous measurement of phagocytic activity of human neutrophils and monocytes in whole blood, the fluorescence capability of the well-known vital stain, neutral red was used. The incubation of 0.5 ml heparinized human blood with the phagocytic stimulus of zymosan dose- and time-dependently increased the percentage and the red fluorescence intensity of both neutrophils (4.3±1.2 times) and monocytes (2.7±0.7 times) measured cytofluorimetrically. Decreased uptake of neutral red was observed in a patient with phagocytic disorder, based upon impaired engulfment of particles and production of reactive oxygen species. In a patient with chronic granulomatosis, however, no decrease of neutral red uptake was measured. Platelet activating factor and phorbol myristate acetate were also able to increase the uptake of neutral red by both monocytes and neutrophils, but to a lesser extent than zymosan. The advantage of this method is the possibility for the simultaneous measurement of phagocytic activities of monocytes and neutrophils stimulated by either particles or soluble activators. This method is suitable for the selective measurement of activation processes not related to the production of free radicals in the phagocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01784045
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Flow cytometric assay of phagocytic activity of human neutrophils and monocytes in whole blood by neutral red uptake (1995)
    Springer
    Annals of hematology 70 (1995), S. 259-265 
    Details
    ISSN: 1432-0584
    Keywords: Key words Phagocytosis ; Neutrophils ; Monocytes ; Flow cytometry ; Neutral red
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  In a new, simple, and fast flow-cytometric method for the simultaneous measurement of phagocytic activity of human neutrophils and monocytes in whole blood, the fluorescence capability of the well-known vital stain, neutral red was used. The incubation of 0.5 ml heparinized human blood with the phagocytic stimulus of zymosan dose- and time-dependently increased the percentage and the red fluorescence intensity of both neutrophils (4.3±1.2 times) and monocytes (2.7±0.7 times) measured cytofluorimetrically. Decreased uptake of neutral red was observed in a patient with phagocytic disorder, based upon impaired engulfment of particles and production of reactive oxygen species. In a patient with chronic granulomatosis, however, no decrease of neutral red uptake was measured. Platelet activating factor and phorbol myristate acetate were also able to increase the uptake of neutral red by both monocytes and neutrophils, but to a lesser extent than zymosan. The advantage of this method is the possibility for the simultaneous measurement of phagocytic activities of monocytes and neutrophils stimulated by either particles or soluble activators. This method is suitable for the selective measurement of activation processes not related to the production of free radicals in the phagocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01784045
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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