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  • 1
    Electronic Resource
    Electronic Resource
    The relationship between wound-induced proteinase inhibitors and hydraulic signals in tomato seedlings (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 17 (1994), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Localized wounding is known to induce systemic proteinase inhibitors (PI) in seedlings of tomato (Lycopersicon esculentum L.). Inhibitors of elastase (EC 3.4.21.36) were shown here to be among those systemically induced by wounding, and a simple rapid assay for PI based on elastase was developed. Using this assay, the nature of the systemic signalling system (‘PIIF’) was investigated. Hydraulic signals were shown to be induced in tomato by localized wounds. These signals travelled throughout the plant well within the lag time before appearance of systemic wound-induced PI. A number of correlations were drawn between the occurrence of the hydraulic signals and induction of systemic PI, suggesting that hydraulic signals might be the PIIF, or a component of it. It was shown that systemic hydraulic signals could be triggered, without significant wounding, by excision of a single leaflet through the submerged petiole. These hydraulic signals were similar in both kinetics and magnitude to those induced by localized wounding. However, they did not induce systemic PI. In addition, it was shown that systemic events almost as rapid as wound-induced hydraulic signals could be induced without wounding, under certain environmental conditions. This indicates that rapid hydraulic signals do not provide a specific signal of wounding. These findings demonstrate that hydraulic signals per se are not the PIIF.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1994.tb00268.x
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  • 2
    Electronic Resource
    Electronic Resource
    Reversed-phase high-performance liquid chromatography of human haemoglobin chains
    Amsterdam : Elsevier
    Journal of Chromatography A 321 (1985), S. 407-419 
    Details
    ISSN: 0021-9673
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0021-9673(01)90459-5
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  • 3
    Electronic Resource
    Electronic Resource
    The bone-ligament junction: A comparison between biological and artificial ACL reconstruction (1993)
    Springer
    Knee surgery, sports traumatology, arthroscopy 1 (1993), S. 9-12 
    Details
    ISSN: 1433-7347
    Keywords: Knee ; anterior cruciate ligament junction ; Biological artificial reconstruction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Sports Science
    Notes: Abstract The physiological bone-ligament junction is composed of four zones: ligament, fibrocartilage, calcified fibrocartilage and bone. It plays a very important part in the distribution of mechanical loads applied to ligaments so as to diminish stress concentration or shearing at the interface. This paper examines types of bone and neoligament insertion after anterior cruciate ligament (ACL) reconstruction with a Dacron prothesis, the Leeds-Keio scaffold ligament (LK), patellar tendon with LAD augmentation (PT+LAD) and bone patellar tendon bone alone (PT). The anterior cruciate reconstructions were implanted in 16 sheep via double-isometric bone tunnels without postoperative knee immobilization. Histological examination of the new insertions (using haematoxylin-cosin, Giemsa, Masson, and Mallory stains) was performed following animal sacrifice after 2, 3, 6 and 9 months. A layer of fibrocartilage between the bone and the ligament was observed with PT, followed by a nearly normal insertion after 6 months. With PT, followed by PT+LAD, the augmentation was surrounded by fibrous tissue (also noted inside the LAD). The PT insertion was virtually physiological after 3–6 months. With the LK scaffold, fibrous tissue was noted in and around the scaffold, even after 6 and 9 months. With the Dacron prosthesis, fibrous tissue around the ligament was unaccompanied by ingrowth into the prosthesis. Nerve endings (pacinian corpuscles) were only present in the PT. These findings show that even after 9 months artificial ligaments are separated from bone by fibrous tissue and devoid of the histological and biomechanical features of a physiological junction. PT alone was the only technique that resulted in formation of a structure very similar to the physiological junction, capable of protecting the bone against excessive shearing stress and the tendon against excessive strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01552151
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  • 4
    Electronic Resource
    Electronic Resource
    Magnetic resonance imaging versus arthroscopy for the investigation of the osteochondral humeral defect in anterior shoulder instability (1995)
    Springer
    Knee surgery, sports traumatology, arthroscopy 3 (1995), S. 184-186 
    Details
    ISSN: 1433-7347
    Keywords: Hill-Sachs lesion ; Magnetic resonance imaging ; Arthroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Sports Science
    Notes: Abstract A double-blind prospective study was done with 15 patients with anterior shoulder instability to determine the diagnostic efficacy of magnetic resonance (MR) imaging versus arthroscopy in the evaluation of chondral or osteochondral lesions of the humeral head. MR produced 6 true positives, 5 true negatives and 4 false negatives, and its accuracy and sensitivity were 60% and 87%, respectively, whereas arthroscopy gave 8 true positives, 5 true negatives and 2 false negatives, with a sensitivity of 80% and an accuracy of 87%. All lesions diagnosed with either method were regarded as positive by definition, with the result that the specificity was always 100%. The differences in diagnosis sprang from the false negatives. The 40% discrepancy between the two methods was probbly due to our distinction in MR between intra- and extraarticular osteochondral lesions. In the first group (the 4 MR false negatives), there were three instances of firstdegree intra-articular lesion and one diagnostic error (third-degree lesion). In the second (the 2 arthroscopy false negatives), the lesions were of the extra-articular type. It is thus advisable to employ both of these methods to ensure the correct diagnosis of a Hill-Sachs lesion, and hence the correct choice of treatment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01565481
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  • 5
    Electronic Resource
    Electronic Resource
    Graft-tunnel mismatch in endoscopic anterior cruciate ligament reconstruction Intraoperative and cadaver measurement of the intra-articular graft length and the length of the patellar tendon (1998)
    Springer
    Knee surgery, sports traumatology, arthroscopy 6 (1998), S. 165-168 
    Details
    ISSN: 1433-7347
    Keywords: Key words Anterior cruciate ; ligament reconstruction ; Intra-articular graft length ; Patellar ; tendon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Sports Science
    Notes: Abstract The results of a study conducted on 50 knees endoscopically reconstructed for an anterior cruciate ligament (ACL) lesion with a free bone-patellar tendon-bone graft and 9 cadaver knees are reported. The mean lengths of the patellar tendon (45.48 ± 4.71 mm) and intra-articular ACL graft (20.44 ± 1.98 mm) were measured in the operated knees. The mean length of the tibial bone tunnel (51.62 ± 2.60 mm) was also measured with a tibial guide at 55°. No statistically significant correlation was found between these three measurements. The length of the patellar tendon was weakly correlated with body height. Measurement of the tibial tunnel on the cadaver knees with increasing degrees of inclination revealed a mean length increase of 0.68 mm per degree (confidence limits: 0.49–0.86). Comparison between the tunnel lengths obtained with the guide and those measured with a Kirschner wire showed a mean difference of 2.3 mm. It is thus desirable to make the tunnel about 53 mm long to ensure excellent fixation of a 28 mm bone block with a 25 mm interference screw. Correct measurement of the anatomical structures involved is in any event an essential requirement for proper execution of the surgical technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001670050093
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  • 6
    Electronic Resource
    Electronic Resource
    Long-term results of the Leeds-Keio anterior cruciate ligament reconstruction (1995)
    Springer
    Knee surgery, sports traumatology, arthroscopy 3 (1995), S. 75-77 
    Details
    ISSN: 1433-7347
    Keywords: Knee ; ACL reconstruction ; Artificial ligament
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Sports Science
    Notes: Abstract This paper discusses the long-term results of the anterior cruciate ligament (ACL) reconstruction with the Leeds-Keio (LK) prosthetic ligament. For this type of reconstruction we used arthrotomy and an arthroscopy-assisted technique. The fixation was obtained with two bone plugs, and the distal portion was also attached with a staple. A postoperative protocol was used with a progressive range of motion and weight bearing after 50 days. We performed 50 LK operations in professional and amateur athletes aged 17–39 years with an isolated anterior instability. We reviewed at follow-up (5–7 years) 37 patients; 8 were lost, and 5 had a subsequent failure. At the Lysholm score the patients were classified: 19 excellent, 13 good, 3 fair, and 2 poor. At the IKDC grading the patients were classified as follows: 2 class A, 22 B, 8 C, and 5 D. The Lachman test was 1+ in 15 patients, 2+ in 7, 3+ in 2, and negative in 13: pivot shift was 1+ in 9, 2+ in 7, 3+ in 2, and negative in 25. Results of the KT 1000 test at 30 Ib side to side was 〈3 mm in 23 patients, 3–5 mm in 6, 6–10 mm in 6, and 〉10 mm in 2. In view of the results observed and the progressive deterioration over the years, this procedure should no longer be performed as an ACL substitute.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01552378
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