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  • 1
    Electronic Resource
    Electronic Resource
    Microbial composition and characterization of prevalent methanogens and acetogens isolated from syntrophic methanogenic granules (1992)
    Springer
    Applied microbiology and biotechnology 38 (1992), S. 282-290 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The microbial species composition of methanogenic granules developed on an acetate-propionate-butyrate mixture was characterized. The granules contained high numbers of adhesive methanogens (1012/g dry weight) and butyrate-, isobutyrate-, and propionate-degrading syntrophic acetogens (1011/g dry weight), but low numbers of hydrolytic-fermentative bacteria (109/g dry weight). Prevalent methanogens in the granules included: Methanobacterium formicicum strain T1N and RF, Methanosarcina mazei strain T18, Methanospirillum hungatei strain BD, and a non-filamentous, bamboo-shaped rod species, Methanothrix/Methanosaeta-like strain M7. Prevalent syntrophic acetogens included: a butyrate-degrading Syntrophospora bryantii-like strain BH, a butyrate-isobutyrate degrading non-spore-forming rod, strain IB, a propionate-degrading sporeforming oval-shaped species, strain PT, and a propionate-degrading none-spore-forming sulfate-reducing rod species, strain PW, which was able to grow syntrophically with an H2-utilizing methanogen. Sulfate-reducing bacteria did not play a significant role in the metabolism of H2, formate, acetate and butyrate but they were involved in propionate degradation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00174484
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Microbial subpopulations in the biofilm attached to the substratum and in the free flocs of a fixed-bed anaerobic bioreactor (1996)
    Springer
    Applied microbiology and biotechnology 46 (1996), S. 443-449 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The microbial flora of a fixed-bed anaerobic methanogenic bioreactor fed with acetate/propionate/butyrate was studied by direct, qualitative and quantitative methods avoiding culture isolation. The aims were to identify species, determine the distribution of microbes between the biofilm attached to the substratum and the free flocs, and define the acidogenic, acetogenic and methanogenic contingents. Optical and scanning electron microscopies showed heterogeneous assemblies of microbes in the biofilm and flocs, which were confirmed by antigenic fingerprinting. A diversity of species involved in the three phases of methanogenesis was detected, and most of these species were antigenically different from the reference organisms. Some microbial subpopulations identified by antigenic fingerprinting changed in size within an interval of 3 weeks, i.e. they either increased or decreased their concentrations by at least tenfold, while others remained relatively constant. The total cell concentration in the flocs was lower than in the biofilm, but at least one microbial subpopulation was more concentrated in the former than in the latter, indicating a preference of location between the compartments available within the bioreactor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00166243
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Microbial subpopulations in the biofilm attached to the substratum and in the free flocs of a fixed-bed anaerobic bioreactor (1996)
    Springer
    Applied microbiology and biotechnology 46 (1996), S. 443-449 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The microbial flora of a fixed-bed anaerobic methanogenic bioreactor fed with acetate/propionate/butyrate was studied by direct, qualitative and quantitative methods avoiding culture isolation. The aims were to identify species, determine the distribution of microbes between the biofilm attached to the substratum and the free flocs, and define the acidogenic, acetogenic and methanogenic contingents. Optical and scanning electron microscopies showed heterogeneous assemblies of microbes in the biofilm and flocs, which were confirmed by antigenic fingerprinting. A diversity of species involved in the three phases of methanogenesis was detected, and most of these species were antigenically different from the reference organisms. Some microbial subpopulations identified by antigenic fingerprinting changed in size within an interval of 3 weeks, i.e. they either increased or decreased their concentrations by at least tenfold, while others remained relatively constant. The total cell concentration in the flocs was lower than in the biofilm, but at least one microbial subpopulation was more concentrated in the former than in the latter, indicating a preference of location between the compartments available within the bioreactor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00166243
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Denitrifying and methanogenic bacteria in the biofilm of a fixed-film reactor operated with methanol/nitrate demonstrated by immunofluorescence and microscopy (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 566-571 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  A denitrifying bacterial biofilm population established on a polypropylene substratum of a fixed-film reactor was characterized by microscopy, scanning electron microscopy and immunofluorescence after 120 days of operation. The reactor, operated at pH 7.0, 22°C, and −180 mV with synthetic wastewater containing methanol/nitrate, achieved a denitrification rate of 0.24 mol NO- 3 l-1 day-1 with a removal efficiency for nitrate of 95%–99% at an organic loading rate of 0.325 mol methanol l-1 day-1. The gas produced contained 2%–3% (v/v) methane and 3%–4% (v/v) carbon dioxide in addition to nitrogen. The biofilm contained mainly cells of Methanobrevibacter arboriphilus antigenically related to strain DC, short, flagellated, gram-negatively staining rods of Pseudomonas sp. antigenically related to Pseudomonas stutzeri strain AN11, non-identified pink-pigmented rods and small lemon-shaped cells with mono- and bipolar appendages resembling prosthecate Hyphomicrobium sp. The biofilm analysis provided evidence for a syntrophy between the denitrifying, methylotrophic, bacterial consortium and hydrogenotrophic methanogens, which were identified by antigenic fingerprinting with 17 antibody probes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050452
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  • 5
    Electronic Resource
    Electronic Resource
    Antigenic determinants distinctive of Methanospirillum hungatei and Methanogenium cariaci identified by monoclonal antibodies (1986)
    Springer
    Archives of microbiology 144 (1986), S. 20-24 
    Details
    ISSN: 1432-072X
    Keywords: Methanospirillum hungatei ; Methanogenium cariaci ; Monoclonal antibodies ; Distinctive antigenic determinants ; JF1 strain sheath ; JR1c strain S layer ; Slide immunoenzymatic assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Monoclonal antibodies were prepared against two species of Methanomicrobiaceae. Antibody 1A is specific for Methanospirillum hungatei strain JF1 and the determinant it recognizes is expressed on the surface of JF1 cells, where it is exposed and accessible to antibody. The determinant is found in a polypeptide (MW〈12,000) in the sheath that covers the bacterial cell; it is not present in Methanospirillum hungatei strain GP1; and it is not expressed on the surface of whole cells of the other 24 methanogenic bacteria tested. It is therefore a marker of strain JF1, consequently, antibody 1A is potentially useful for tracking JF1 and fragments thereof in a variety of samples. Antibody 7A is specific for Methanogenium cariaci JR1c. It did not react with any other methanogen tested, not even with Mg. marisnigri or Ms. hungatei JF1, although these cross-react with Mg. cariaci if tested with polyclonal antisera. Therefore antibody 7A recognizes specifically a marker of Mg. cariaci JR1c.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00454950
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  • 6
    Electronic Resource
    Electronic Resource
    Characterization of a new mesophilic, secondary alcohol-utilizing methanogen, Methanobacterium palustre spec. nov. from a peat bog (1988)
    Springer
    Archives of microbiology 151 (1988), S. 1-9 
    Details
    ISSN: 1432-072X
    Keywords: Archaebacteria ; Methanobacterium palustre spec. nov. ; Secondary alcohol utilization ; Secondary alcohol dehydrogenase ; NADP+ ; Serological probes ; Polyamines ; Polar lipids ; Chemotaxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The isolation and characterization of a new methanogen from a peat bog, Methanobacterium palustre spec. nov., strain F, is described. Strain F grew on H2/CO2 and formate in complex medium. It also grew autotrophically on H2/CO2. Furthermore, growth on 2-propanol/CO2 was observed. Methane was formed from CO2 by oxidation of 2-propanol to acetone or 2-butanol to 2-butanone, but growth on 2-butanol plus CO2 apparently was too little to be measurable. Similarly, Methanobacterium bryantii M. o. H. and M. o. H. G formed acetone and 2-butanone from 2-propanol and 2-butanol, but no growth was measurable. On the basis of morphological and biochemical features strain F could be excluded from the genus Methanobrevibacter. Due to its cell morphology, lipid composition and polyamine pattern it belonged to the genus Methanobacterium. From known members of this genus strain F could be distinguished either by a different G+C content of the DNA, low DNA-DNA homology with reference strains, lacking serological reactions with anti-S probes and differences in the substrate spectrum. An alcohol dehydrogenase activity, specific for secondary alcohols and its substrate specificity was determined in crude extracts of strain F. NADP+ was the only electron carrier that was utilized. No reaction was found with NAD+, F420, FMN and FAD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00444660
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