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  • 1
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Synthesis of acetylcholine depends on the plasma membrane uptake of choline by a high affinity choline transporter (CHT1). Choline uptake is regulated by nerve impulses and trafficking of an intracellular pool of CHT1 to the plasma membrane may be important for this regulation. We have generated a hemagglutinin (HA) epitope tagged CHT1 to investigate the organelles involved with intracellular trafficking of this protein. Expression of CHT1-HA in HEK 293 cells establishes Na+-dependent, hemicholinium-3 sensitive high-affinity choline transport activity. Confocal microscopy reveals that CHT1-HA is found predominantly in intracellular organelles in three different cell lines. Importantly, CHT1-HA seems to be continuously cycling between the plasma membrane and endocytic organelles via a constitutive clathrin-mediated endocytic pathway. In a neuronal cell line, CHT1-HA colocalizes with the early endocytic marker green fluorescent protein (GFP)-Rab 5 and with two markers of synaptic-like vesicles, VAMP-myc and GFP-VAChT, suggesting that in cultured cells CHT1 is present mainly in organelles of endocytic origin. Subcellular fractionation and immunoisolation of organelles from rat brain indicate that CHT1 is present in synaptic vesicles. We propose that intracellular CHT1 can be recruited during stimulation to increase choline uptake in nerve terminals.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Langenbeck's archives of surgery 346 (1978), S. 265-271 
    ISSN: 1435-2451
    Keywords: Head injury ; Muscular ossification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wird über 14 Patienten mit einer Myositis ossificans bei gleichzeitigem Schädel-Hirntrauma berichtet. Bevorzugte Lokalisationen sind das Hüft- und Ellbogengelenk sowie die Oberschenkelmuskulatur, wo besonders bei Jugendlichen und jüngeren Erwachsenen 5–8 Wochen nach dem Unfall die Verknöcherungen auftreten. Vor einem zu frühen operativen Eingreifen muß wegen der Gefahr des Rezidivs gewarnt werden. Der Prozeß der Verkalkung kommt gewöhnlich 8–12 Wochen nach dem Trauma zum Stillstand. Die klinische Untersuchung (Muskelverhärtung, Einschränkung der Gelenksbeweglichkeit) und das Röntgenbild erlauben uns die Diagnose zu stellen. Pathogenese und Therapiemöglichkeiten werden näher besprochen. Weiterhin werden statistische Untersuchungen zur Häufigkeit dieser Erkrankung bei Schädel-Hirntraumen in den einzelnen Dezennien auf Grund von Angaben aufgeführt.
    Notes: Summary This is a report on 14 patients with myositis ossificans with simultaneous head injury. Predilected sites are the hip and elbow joints as well as thigh musculature, where particularly in the youthful and younger adults ossifications occur 5–8 weeks after the accident. Premature operative treatment is useless because of the danger of recurrence. The process of ossification usually comes to a standstill 8–12 weeks following the trauma. The diagnosis is made on the basis of clinical examination (hardening of muscles, immobility of joints) and X-ray pictures. The pathogenesis and modes of therapy are discussed in detail. Furthermore, the incidence of this complication of head injury in various age groups is evaluated statistically.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Langenbeck's archives of surgery 349 (1979), S. 582-582 
    ISSN: 1435-2451
    Keywords: Reflux oesophagitis ; Ligamentum teres ; Oesophageal traction ; Refluxoesophagitis ; Ligamentum teres ; Längsspannung des Oesophagus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei 29 Patienten mit Refluxoesophagitis bei Gleithernie führten wir eine als Teresplastik bezeichnete Operation durch. Dabei wurde nach Oberbauchmedianschnitt ein handflächengroßes Bauchfellblatt, das in das Ligamentum teres sowie in das Ligamentum falciforme übergeht, zu einem Strang vernäht und anschließend zwischen Fundus und distalen Oesophagus genäht. Kontrollmanometrie und pH-Metrie ergaben normalisierte Werte. Das Prinzip des Verfahrens besteht in der dauernden Wiederherstellung der Oesophaguslängsspannung.
    Notes: Summary A so-called “Teresplasty” was performed on 29 patients presenting with a sliding hiatus hernia and accompanying reflux oesophagitis. A upper median abdominal incision was performed and an area of peritoneum - the size of the palm of the hand - including the ligamentum teres and adjoining the falciform ligament was prepared and sutured, forming a band-like tissue structure. Its free end was then fixed between the stomach fundus and the distal part of the oesophagus. Postoperative manometry and pH investigation produced normal values. The aim of the technique is to restore definitely the longitudinal downward oesophageal traction.
    Type of Medium: Electronic Resource
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