ISSN:
1432-0991
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The protoplasting and transformation techniques described by Chang and Cohen [5] have been modified by the inclusion of mutanolysin and these techniques have been used to prepare protoplasts of a number ofBacillus spp. Cells of some, however, remained resistant to cell wall hydrolysis by both mutanolysin and lysozyme. Protoplasts were prepared from sixBacillus species, including a strain ofB. subtilis, and transformed with the plasmid pUB110. Transformation with the shuttle vector pHV33 was, however, less successful and antibiotic-resistant protoplasts, although detected, either failed to regenerate their osmotic stability or rapidly lost their antibiotic resistance.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01568945
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