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  • 1
    Electronic Resource
    Electronic Resource
    Synthesis and turnover of Euglena gracilis nuclear and chloroplast deoxyribonucleic acid (1972)
    s.l. : American Chemical Society
    Biochemistry 11 (1972), S. 2036-2043 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00761a007
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  • 2
    Electronic Resource
    Electronic Resource
    Kinetoplast DNA of Crithidia (1974)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 21 (1974), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. The results of physiochemical and electron microscope studies on the structure of the kinetoplast DNA of Crithidia are discussed. The molecular weight of isolated kinetoplast DNA is approximately 41 × 109 daltons. The basic unit of organization of this DNA is a covalently closed circular molecule 0.8 μm in contour length (molecular weight 1.54 × 106 daltons). An average of 33 such circles are held together in a group by topological interlocking of each circle with a large number of other circles in the group. An average of 804 groups are held together into a structure termed an association. A group of circles is attached to several adjacent groups. This is accomplished by 1 or more circles each interlocking with many circles in 2 (but never more) of the groups. The groups of circles are arranged in such a way that the association, comprising about 27,000 circles, has a topologically 2-dimensional form. A model is presented for the in situ arrangement of the kinetoplast DNA association. Data which we have obtained is consistent with the view that all of the circular molecules of kinetoplast DNA of one organism carry the same genetic information. The transcription product is a single RNA molecule of approximately the size of a single circular DNA molecule. From studies involving density shift experiments using deuterium oxide, buoyant density ultracentrifugation and electron microscopy we have determined the following concerning replication of kinetoplast DNA: (a) doubling of the kinetoplast DNA results from the replication of each circular molecule rather than from extensive replication of a small number of circular molecules; (b) replication of kinetoplast DNA is a semi-conservative rather than a conservative process and there is recombination at an undefined time during the cell cycle; (c) a portion of a kinetoplast DNA association is in the form of long linear molecules during the replicative phase of that association.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1974.tb03716.x
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  • 3
    Electronic Resource
    Electronic Resource
    DNA sequence organization in theThysanura Thermobia domestica (1980)
    Springer
    Journal of molecular evolution 15 (1980), S. 277-289 
    Details
    ISSN: 1432-1432
    Keywords: DNA sequence organization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cot and chemical analysis show that the haploid genome size of Thermobia domestica is 3–4×109 nucleotide pairs. Of this DNA 33% is single copy sequences and 67% is repetitive sequences. The repetitive sequences are predominantly 300 nucleotides in length and are interspersed among the single copy sequences in a short period interspersion pattern similar to that observed in Xenopus and many other higher eucaryotes. The DNA sequence organization observed in Thermobia is compared with that of other more highly evolved insects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01733135
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  • 4
    Electronic Resource
    Electronic Resource
    Cloning of a major surface-antigen gene of Trypanosoma cruzi and identification of a nonapeptide repeat (1986)
    [s.l.] : Nature Publishing Group
    Nature 322 (1986), S. 566-568 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We have previously reported that the major surface proteins of the trypomastigote stage can be isolated free of most cytosolic components by using iminobiotin-avidin interaction6. This preparation of iminobiotinylated surface proteins (IBSP) containsthe major 85K and 90K glycoproteins as well as ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/322566a0
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  • 5
    Electronic Resource
    Electronic Resource
    A new method of in situ hybridization (1975)
    Springer
    Chromosoma 53 (1975), S. 107-117 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A new method for gene mapping at the chromosome level using in situ hybridization and scanning electron microscopy is described and has been applied to mapping the rRNA genes of Drosophila melanogaster. Biotin is covalently attached to Drosophila rRNA via a cytochrome c bridge at a ratio of one cytochrome-biotin per 130 nucleotides by a chemical procedure. Polymethacrylate spheres with a diameter of ca. 60 nm are prepared by emulsion polymerization and are covalently attached to the protein avidin at a ratio of 5–20 avidins per sphere. The biotin-labeled rRNA is hybridized to denatured DNA in a chromosome squash. Upon incubation with a sphere solution, some of the biotin sites become labeled with spheres because of the strong non-covalent interaction between biotin and avidin. The chromosome squash is examined in the scanning electron microscope (SEM). Polymer spheres, which are visible in the SEM, are observed to label the nucleolus, where the rRNA genes are located.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333039
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  • 6
    Electronic Resource
    Electronic Resource
    The non-dosage compensatedLSPI -α gene ofDrosophila melanogaster lies immediately downstream of the dosage compensatedL12 gene (1992)
    Springer
    Molecular genetics and genomics 233 (1992), S. 49-52 
    Details
    ISSN: 1617-4623
    Keywords: Dosage compensation ; LSPI-α ; L12 ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The X-linked geneLSPI-α ofDrosophila melanogaster, expressed in the third larval instar, does not exhibit dosage compensation at its normal locus but does compensate when it is relocated to ectopic sites on the X chromosome. A transcription unit designatedL12, which is active in the second larval instar and capable of encoding a putative protein of 28.5 kDa, lies immediately downstream fromLSPI-α. We have determined thatL12 is dosage compensated by measuring the steady-state level of its transcript in male and female larvae. The difference in response of these two adjacent genes should be taken into consideration when models of the mechanism of dosage compensation are formulated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00587560
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  • 7
    Electronic Resource
    Electronic Resource
    Cloning and dosage compensation of the 6-phosphogluconate dehydrogenase gene (Pgd+) of Drosophila melanogaster (1989)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 10 (1989), S. 155-161 
    Details
    ISSN: 0192-253X
    Keywords: 6PGD ; 6-phosphogluconate dehydrogenase ; X chromosome ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Using a heterologous rat cDNA probe, we have identified a 14.7 kbp Drosophila melanogaster genomic clone containing the X-linked gene Pgd+, which encodes the enzyme 6-phosphogluconate dehydrogenase (6PGD). We used in situ hybridization to larval polytene chromosomes, a somatic transient expression assay for enzyme activity, and the rescue of the lethal Pgd- phenotype by germline transformation to verify the identity of the gene. A 7.4 kbp fragment including the gene and approximately 1.2 kbp of upstream and 1.8 kbp of downstream sequences was relocated to autosomal ectopic sites by germline transformation; this transduced gene exhibits levels of enhanced activity in males comparable to those of the indigenous gene at its normal X chromosome locus. We conclude that the sequences responsible for dosage compensation of Pgd+ are included in this fragment.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020100305
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