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  • 1
    Electronic Resource
    Electronic Resource
    Solid immersion microscope (1990)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 57 (1990), S. 2615-2616 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A new type of real-time optical microscope has been developed that operates on the same principle as a liquid immersion microscope, with the liquid replaced by a solid lens of high refractive index material. Using a lens with an index n=2 and 436 nm illumination, this microscope has resolved 100 nm lines and spaces and has demonstrated a factor of two improvement in the edge response over a confocal microscope.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.103828
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  • 2
    Electronic Resource
    Electronic Resource
    A Phosphorylation Epitope on MAP 1B that is Transiently Expressed in Growing Axons in the Developing Rat Nervous System (1993)
    Oxford, UK : Blackwell Publishing Ltd
    European journal of neuroscience 5 (1993), S. 0 
    Details
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We have isolated a monoclonal antibody (150) that recognizes a phosphorylation epitope on the microtubule-associated protein (MAP) 1B. Immunoblot analysis of the developing rat central nervous system shows that monoclonal antibody 150 is directed against a protein of ∼325 kDa (MAP 1B) that copolymerizes with microtubules through successive cycles of temperature-dependent assembly and disassembly. Furthermore, immunoprecipitated MAP 1B contains the epitope recognized by monoclonal antibody 150. Removal of phosphate from blotted proteins using alkaline phosphatase abolishes the binding of monoclonal antibody 150 to MAP 1B, indicating that the epitiope is phosphorylated. In the developing rat nervous system, immunohistochemistry with monoclonal antibody 150 shows that the phosphorylation epitope on MAP 1B is transiently expressed in growing axons but not in dendrites. For instance, in the neonatal rat cerebellum, the parallel fibres of granule cells are stained only during elongation and not after synaptogenesis. The monoclonal antibody 150 epitope is also transiently expressed in radial glial fibres and in certain cell nuclei. All immunostaining of sections with monoclonal antibody 150 was completely abolished by alkaline phosphatase treatment. These observations and previous ones made by us in cell culture (Mansfield et al., J. Neurocytol., 20, 654 – 666, 1991) suggest that the phosphorylation epitope on MAP 1B recognized by monoclonal antibody 150, which has not been previously detected in vivo, may be important in axonogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1460-9568.1993.tb00916.x
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  • 3
    Electronic Resource
    Electronic Resource
    Computers across the curriculum: A confluence of ideas
    Amsterdam : Elsevier
    Computers and Composition 6 (1988), S. 9-13 
    Details
    ISSN: 8755-4615
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Computer Science , Education
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S8755-4615(88)80023-9
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  • 4
    Electronic Resource
    Electronic Resource
    Computers across the curriculum: A confluence of ideas
    Amsterdam : Elsevier
    Computers and Composition 6 (1988), S. 9-13 
    Details
    ISSN: 8755-4615
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Computer Science , Education
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S8755-4615(88)80023-9
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  • 5
    Electronic Resource
    Electronic Resource
    Discharge of gonadotrophin-releasing hormone from the mediobasal part of the hypothalamus: Effect of stimulation frequency and gonadal steroids (1980)
    Springer
    Experimental brain research 39 (1980), S. 453-460 
    Details
    ISSN: 1432-1106
    Keywords: Gonadotrophin-releasing hormone ; Hypothalamic stimulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The release of gonadotrophin-releasing hormone (Gn-RH), in response to electrical stimulation of the mediobasal part of the hypothalamus incubated in vitro, was studied in both male and female rats. In male rats significant release of Gn-RH occurred during the 10-min experimental period only when the incubated tissue was stimulated at frequencies of 10 Hz or greater. There was no release when stimulated at 5 Hz. There was also no release of hormone when the mediobasal hypothalami were incubated in a Ca2+ free medium. The amount of Gn-RH released during a 10-min incubation period increased progressively as the frequency of stimulation was raised from 10–100 Hz. During short (4-min) incubation periods the effectiveness of each stimulus pulse for Gn-RH release also increased with the frequency of stimulation. However, when stimulated for 10 min there was no increase in hormone released per stimulus pulse when frequency of stimulation was raised above 10 Hz. The amount of Gn-RH released in response to stimulation at 50 Hz was greater in male rats than in females. For the females, there was no significant difference between the amounts of Gn-RH released at dioestrus and pro-oestrus. In both male and female rats gonadectomised 4 weeks prior to hypothalamic incubation, the response to electrical stimulation at 50 Hz was reduced when compared with intact controls. Indeed, for the females there was no longer a statistically significant increase in the amount of Gn-RH in the incubation medium after 50 Hz stimulation. Ovariectomised female rats, injected twice daily for 3.5 days with 20 μg of oestradiol benzoate released Gn-RH in response to 50 Hz stimulation in the same amounts as intact control animals. By contrast, there was no recovery of Gn-RH release to normal levels in castrated male rats similarly treated with 1.25 mg testosterone propionate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00239310
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  • 6
    Electronic Resource
    Electronic Resource
    Partial correction of endogenous ΔF508 CFTR in human cystic fibrosis airway epithelia by spliceosome-mediated RNA trans -splicing (2002)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 20 (2002), S. 47-52 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Spliceosome-mediated RNA trans-splicing (SMaRT) was investigated as a means for functionally correcting endogenous ΔF508 cystic fibrosis transmembrane conductance regulator (CFTR) transcripts using in vitro human cystic fibrosis (CF) polarized airway epithelia and in vivo human CF bronchial ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0102-47
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  • 7
    Electronic Resource
    Electronic Resource
    Spliceosome-mediated RNA trans -splicing as a tool for gene therapy (1999)
    [s.l.] : Nature America Inc.
    Nature biotechnology 17 (1999), S. 246-252 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] We have developed RNA molecules capable of effecting spliceosome-mediated RNA trans-splicing reactions with a target messenger RNA precursor (pre-mRNA). Targeted trans-splicing was demonstrated in a HeLa nuclear extract, cultured human cells, and H1299 human lung cancer tumors in athymic mice. ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/6986
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  • 8
    Electronic Resource
    Electronic Resource
    Phenotype correction of hemophilia A mice by spliceosome-mediated RNA trans -splicing (2003)
    [s.l.] : Nature Publishing Group
    Nature medicine 9 (2003), S. 1015-1019 
    Details
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Conventional gene therapy of hemophilia A relies on the transfer of factor VIII (FVIII; encoded by the F8 gene) cDNA. We carried out spliceosome-mediated RNA trans-splicing (SMaRT) to repair mutant FVIII mRNA. A pre-trans-splicing molecule (PTM) corrected endogenous FVIII mRNA in F8 knockout mice ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nm900
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  • 9
    Electronic Resource
    Electronic Resource
    Xylanase prebleaching of fractions of Douglas-fir kraft pulp of different fibre length (1996)
    Springer
    Applied microbiology and biotechnology 46 (1996), S. 319-326 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A commercial xylanase from Trichoderma longibrachiatum was used to treat the fractions of Douglas-fir kraft pulp of different fibre length. Enzymatic prebleaching was followed by chelation and peroxide bleaching. An evaluation of both optical and physical properties of the distinct fractions was conducted. A difference in susceptibility of the fractions of different fibre length to xylanase prebleaching was observed. The bleach-boosting effect observed with all fractions appeared to be related to the high-molecular-mass UV-absorbing material solubilized during enzyme treatments. Xylanase treatments resulted in beneficial effects to handsheet density and roughness as well as to some of the strength properties. However, the response to the xylanase treatments exhibited by all fractions of different fibre length was not uniform, indicating that fiber composition played a key role in the effectiveness of xylanase treatments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00166224
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  • 10
    Electronic Resource
    Electronic Resource
    Xylanase prebleaching of fractions of Douglas-fir kraft pulp of different fibre length (1996)
    Springer
    Applied microbiology and biotechnology 46 (1996), S. 319-326 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  A commercial xylanase from Trichoderma longibrachiatum was used to treat the fractions of Douglas-fir kraft pulp of different fibre length. Enzymatic prebleaching was followed by chelation and peroxide bleaching. An evaluation of both optical and physical properties of the distinct fractions was conducted. A difference in susceptibility of the fractions of different fibre length to xylanase prebleaching was observed. The bleach-boosting effect observed with all fractions appeared to be related to the high-molecular- mass UV-absorbing material solubilized during enzyme treatments. Xylanase treatments resulted in beneficial effects to handsheet density and roughness as well as to some of the strength properties. However, the response to the xylanase treatments exhibited by all fractions of different fibre length was not uniform, indicating that fiber composition played a key role in the effectiveness of xylanase treatments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00166224
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    Paper (German National Licenses)
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