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  • 1
    Electronic Resource
    Electronic Resource
    Expression of the two chloroplast-like tRNAAsn genes in potato mitochondria: mapping of transcription initiation sites present in the trnN1-trnY-nad2 cluster and upstream of trnN2 (1999)
    Springer
    Current genetics 36 (1999), S. 49-54 
    Details
    ISSN: 1432-0983
    Keywords: Key words Chloroplast-like tRNA gene ; nad2 ; Plant mitochondrial promoter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two copies of the chloroplast-like tRNAAsn gene, trnN1 and trnN2, are expressed in potato mitochondria. While Northern-blot analysis revealed only mature tRNAAsn, RT-PCR indicated that trnN1 is co-transcribed with trnY and nad2 (exons c, d and e). Using primer-extension and capping experiments, four transcription initiation sites have been mapped in the vicinity of these genes. The first site, responsible for the co-transcription of trnN1, trnY and nad2 (exons c, d and e), gives rise to a primary transcript of at least 7000 nt. A second site, 58 nt downstream from trnY, corresponds to an alternative promoter specific for nad2. In both cases, only the CRTA core motif of the consensus CRTAaGaGA of dicot mitochondrial promoters was found. Finally, two transcription initiation sites were identified 135 and 128 nt upstream of trnN2 in a region which shows no sequence homology with this consensus motif.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050471
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of some major identity elements in plant alanine and phenylalanine transfer RNAs (1994)
    Springer
    Plant molecular biology 26 (1994), S. 1843-1853 
    Details
    ISSN: 1573-5028
    Keywords: aminoacylation ; identity element ; plant ; suppression ; transfer RNA ; transient expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Alanine and phenylalanine tRNA sequences were amplified by PCR from Arabidopsis thaliana nuclear DNA using degenerate oligonucleotides which introduced specific mutations into the acceptor stem. The aminoacylation of T7 RNA polymerase transcripts of these sequences was investigated in vitro using partially purified bean alanyl- or phenylalanyl-tRNA synthetase. In parallel, the in vivo activity of amber suppressor derivatives of these tRNAs was investigated in transient expression assays in tobacco protoplasts using a β-glucuronidase (GUS) reporter gene containing a premature amber stop codon. The results show that mutation of the G3:U70 base pair to G3:C70 blocks aminoacylation of plant alanine tRNA, whilst conversion of the G3:C70 pair normally found in plant tRNAPhe to G3:U70 enables the mutated tRNAPhe to be a good substrate for analyl-tRNA synthetase and impairs its aminoacylation with phenylalanine. In addition, the amber suppressor derivative of wild-type tRNAPhe showed very little suppressor activity in vivo, and was poorly aminoacylated with phenylalanine in vitro, suggesting that the anticodon is a major identity determinant for tRNAPhe in plant cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019497
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Striking differences in mitochondrial tRNA import between different plant species (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 404-411 
    Details
    ISSN: 1617-4623
    Keywords: Key words Mitochondria ; tRNA ; Import ; Maize ; Potato ; Larch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A systematic comparison of the tRNAs imported into the mitochondria of larch, maize and potato reveals considerable differences among the three species. Larch mitochondria import at least eleven different tRNAs (more than half of those tested) corresponding to ten different amino acids. For five of these tRNAs [tRNAPhe(GAA), tRNALys(CUU), tRNAPro(UGG), tRNASer(GCU) and tRNASer(UGA)] this is the first report of import into mitochondria in any plant species. There are also differences in import between relatively closely related plants; wheat mitochondria, unlike maize mitochondria import tRNAHis, and sunflower mitochondria, unlike mitochondria from other angiosperms tested, import tRNASer(GCU) and tRNASer(UGA). These results suggest that the ability to import each tRNA has been acquired independently at different times during the evolution of higher plants, and that there are few apparent restrictions on which tRNAs can or cannot be imported. The implications for the mechanisms of mitochondrial tRNA import in plants are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173005
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Structure and expression of several bean (Phaseolus vulgaris) nuclear transfer RNA genes: relevance to the process of tRNA import into plant mitochondria (1998)
    Springer
    Plant molecular biology 36 (1998), S. 613-625 
    Details
    ISSN: 1573-5028
    Keywords: identity determinants ; mitochondria ; nuclear genome ; plant ; tRNA genes ; tRNA import
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Bean nuclear genes for tRNAPro, tRNAThrand tRNALeu were isolated. Expression of the tRNAPro genes was demonstrated in vivo and sequence analysis suggested amplification of the tRNAPro gene copy number through duplication of a gene cluster at the same locus of the bean genome. The two tRNAThr genes isolated were actively transcribed and their transcripts processed in a HeLa cell system. In vivo expression tests of these genes and aminoacylation assays of the corresponding in vitro transcripts showed the presence of identity determinants in the anticodon of plant tRNAThr. The tRNALeu gene was not expressed due to deviation from the consensus in the internal B-box promoter. The same sequence deviation also prevented aminoacylation of the corresponding in vitro transcript. This tRNALeu however exists in plants and is synthesized from another gene with a consensus B-box promoter. Plant mitochondria import from the cytosol a number of nucleus-encoded tRNAs, including tRNALeu and tRNAThr. From the available sequence data, we could not identify any conserved structural motif characteristic for the nucleus-encoded tRNAs imported into plant mitochondria, either in the tRNAs, or in the gene flanking sequences. These results suggest that recognition of tRNAs for import is idiosyncratic and likely to depend on protein/RNA interactions that are specific to each tRNA or each isoacceptor group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005972023506
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    Paper (German National Licenses)
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