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  • 1
    Electronic Resource
    Electronic Resource
    MOLECULAR GENETIC ANALYSIS OF TRICHOME DEVELOPMENT IN ARABIDOPSIS (1997)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Plant Physiology and Plant Molecular Biology 48 (1997), S. 137-163 
    Details
    ISSN: 1040-2519
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Notes: Abstract Two basic questions in developmental biology are: How does a cell know when it should or should not differentiate, and once a cell is committed to differentiate, how is that process controlled? The first process regulates the arrangement or pattern of the various cell types, whereas the second makes cells functionally distinct. Together, these two processes define plant morphogenesis. Trichome development in Arabidopsis provides an excellent model to analyze these questions. First, trichome development in Arabidopsis is a relatively simple process. A single epidermal cell differentiates into a unicellular trichome. Second, this differentiation occurs in a nonrandom pattern on the plant surface. Finally, the process is amenable to genetic analysis because many mutations that affect trichome differentiation do not alter other aspects of plant development. Thus far, more than 20 genes affecting trichome development have been identified. This review examines the current state of our understanding of these genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.arplant.48.1.137
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of a weak allele of the GL1 gene of Arabidopsis thaliana (1994)
    Springer
    Plant molecular biology 24 (1994), S. 203-207 
    Details
    ISSN: 1573-5028
    Keywords: trichome ; plant development ; Myb ; transcription factor ; cell expansion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A genomic clone containing the gl1–2 allele has been isolated and sequenced. The predicted amino acid sequence of the gl1–2 protein is identical to that of the GL1-Col allele up to position 201. At this point in the coding region of gl1–2 there is a deletion relative to the wild-type sequence that results in an in-frame stop codon at position 202. This deletion removes 27 amino acid residues, including a highly negatively charged region, from the predicted gl1–2 polypeptide. The loss of this negatively charged carboxy-terminal region from the gl1–2 product is most likely the cause of the partial loss of gene activity which results in a reduction in leaf trichome initiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040586
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The relatively large beta-tubulin gene family of Arabidopsis contains a member with an unusual transcribed 5′ noncoding sequence (1987)
    Springer
    Plant molecular biology 10 (1987), S. 91-104 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; beta-tubulin ; glyceraldehyde-3-phosphate dehydrogenase subunit B ; leader sequence ; multigene family
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have characterized the beta-tubulin gene family of Arabidopsis thaliana. Five distinct genes were cloned and analyzed by restriction enzyme mapping and cross-hybridization studies. Three of the genes appear to be dispersed, whereas two others are linked within 1.5 kb of one another. The two linked genes are closely related and appear to have resulted from a fairly recent duplication. The three dispersed genes do not cross-hybridize to one another or to the two linked genes under highly stringent hybridization conditions, suggesting that they arose from more ancient duplications. From Southern analysis we estimate that there are a total of between six and ten beta-tubulin genes in Arabidopsis. Additional analyses indicate that the gene family is equal in size or larger than those in other plants, but significantly smaller than those in related Brassica species. Sequence determination of one of the Arabidopsis genes revealed a highly unusual transcribed leader sequence. The leader contains two fairly long tracks of adenines. One is located toward the 5′ end of the mRNA and the other is just before the initiation codon. A track of uridines is located between the adenine tracks. This leader can form two different secondary structures that may have regulatory significance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016147
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  • 4
    Electronic Resource
    Electronic Resource
    Molecular cloning and physical characterization of a Brassica linear mitochondrial plasmid (1987)
    Springer
    Molecular genetics and genomics 209 (1987), S. 227-233 
    Details
    ISSN: 1617-4623
    Keywords: Inverted repeats ; Terminally attached protein ; Chloroplast and mitochondrial sequence homology ; Cytoplasmic male sterility ; Ogura eytoplasm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A linear mitochondrial plasmid reported to be associated with cytoplasmic male sterility in the genus Brassica was analyzed. A protein was found to be associated with the 5′ ends of the plasmid. The entire plasmid was cloned by the homopolymer tailing technique via free hydroxyl groups present at its 3′ ends. DNA sequence analysis of the cloned plasmid revealed a perfect terminal inverted repeat of 325 base pairs. Southern hybridization and restriction enzyme mapping analysis confirmed colinearity of the native plasmid and the clone, which showed significant homology with organelle DNA but not with nuclear DNA. Under high-stringency hybridization conditions, an internal 4.6 kb fragment of the 11.5 kb plasmid hybridized to the main mitochondrial genome in several species. Although the hybridization signal was weaker, the chloroplast genome also showed homology to the mitochondrial plasmid. The plasmid was undetectable at a molar ratio of less than 1/10 000 of the main mitochondrial genome in some lines of Brassica and Raphanus that contain the Ogura male sterile cytoplasm (cms). The absence of the plasmid in these sterile lines demonstrates that the plasmid is not required for the expression and maternal inheritance of male sterility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00329647
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    Paper (German National Licenses)
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