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  • 1
    Electronic Resource
    Electronic Resource
    A mutation hotspot in the chloroplast genome of a conifer (Douglas-fir: Pseudotsuga) is caused by variability in the number of direct repeats derived from a partially duplicated tRNA gene (1995)
    Springer
    Current genetics 27 (1995), S. 572-579 
    Details
    ISSN: 1432-0983
    Keywords: Pinaceae ; Length mutation ; Repetitive DNA ; Chloroplast DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We determined the DNA sequence of a 2.7-kb cpDNA XbaI fragment from douglas-fir [Pseudotsuga menziesii (Mirb.) Franco]. RFLPs revealed by the 2.7-kb XbaI clone were observed to vary up to 1 kb among species within the genus Pseudotsuga and up to 200 bp among trees of P. menziesii. The polymerase chain reaction (PCR) allowed the locus of polymorphism to be identified, and the variable region was then sequenced in a second Douglas-fir tree, a single tree of a related species, Japanese Douglas-fir (P. japonica), and in a species lacking a mutation hotspot in the region, Pinus radiata (Monterey pine). The locus of polymorphism is characterized by hundreds of base pairs of imperfect, tandem direct repeats flanked by a partially duplicated and an intact trnY-GUA gene. The duplication is direct in orientation and consists of 43 bp of the 3′ end of trnY and 25 bp of its 3′ flanking sequence. Tandem repeats show high sequence similarity to a 27-bp region of the trnY gene that overlaps one end of the duplication. The two trees of Douglas-fir sequenced differed by a single tandem repeat unit, whereas these trees differed from the Japanese Douglas-fir sequenced by approximately 34 repeat units. Repetitive DNA in the Pseudotsuga cpDNA hotspot was most likely generated at the time of the partial trnY gene duplication and these sequences expanded by slipped-strand mispairing and unequal crossingover.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00314450
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  • 2
    Electronic Resource
    Electronic Resource
    Chloroplast DNA restriction fragment length polymorphism in Sequoia sempervirens D. Don Endl., Pseudotsuga menziesii (Mirb.) Franco, Calocedrus decurrens (Torr.), and Pinus taeda L. (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 83-89 
    Details
    ISSN: 1432-2242
    Keywords: cpDNA RFLPs ; Sequoia sempervirens D. Don Endl. ; Pseudotsuga menziesii (Mirb.) Franco ; Calocedrus decurrens (Torr.) Florin ; Pinus taeda L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The extent and type of chloroplast DNA restriction fragment length polymorphism was determined among individual tree samples of coast redwood, Douglas fir, incense-cedar, and loblolly pine. A total of 107 trees was surveyed for three restriction enzymes (BamHI, EcoRI, HindIII) and six chloroplast DNA probes from petunia (P3, P4, P6, P8, P10, S8). The probes comprise 64% of the petunia chloroplast genome. Polymorphisms were detected in all species but loblolly pine. Coast redwood and incense-cedar had a small number of rare variants, whereas Douglas fir had one highly polymorphic region of insertions/deletions in sequences revealed by the P6 probe from petunia. The mutation hotspot is currently being studied by DNA sequence analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226116
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Use of DNA markers in forest tree improvement research (1992)
    Springer
    New forests 6 (1992), S. 391-407 
    Details
    ISSN: 1573-5095
    Keywords: DNA markers ; genetic maps ; RFLPs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract DNA markers are rapidly being developed for forest trees. The most important markers are restriction fragment length polymorphisms (RFLPs), polymerase chain reaction- (PCR) based markers such as random amplified polymorphic DNA (RAPD), and fingerprinting markers. DNA markers can supplement isozyme markers for monitoring tree improvement activities such as; estimating genetic diversity in breeding populations, germplasm identification, verifying controlled crosses, and estimating seed orchard efficiencies. Because the number of DNA markers is potentially limitless, it should be possible to map individual quantitative trait loci (QTL) by linkage analysis with high-density maps. Finally, if such associations can be found, it may also be possible to design marker-assisted breeding strategies for forest trees.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00120654
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