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  • 1
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The carrier rate of group B beta-haemolytic streptococci (GBS) was assessed in pregnant and non-pregnant women attending four clinics. Multiple sites were sampled and standard methods were used to isolate GBS. Colonization rates were highest in patients attending a venereal disease clinic but were not associated with other sexually transmitted diseases. Higher colonization rates were detected when multiple sites were sampled. Serotypes II and III occurred most frequently.
    Type of Medium: Electronic Resource
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  • 2
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    Philadelphia, etc. : Periodicals Archive Online (PAO)
    Current History. 24:141 (1953:May) 295 
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  • 3
    ISSN: 1432-0983
    Keywords: Neurospora ; Transformation ; LaBelle ; Plasmids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have studied the efficacy of transformation of Neurospora crassa with a chimaeric plasmid. We constructed a recombinant plasmid, pMK2, consisting of the mitochondrial plasmid of N. intermedia LaBelle, a part of the qa gene cluster of Neurospora and the Escherichia coli plasmid pBR322. Compared to plasmid pVK88, not containing the LaBelle sequence, the pMK2 plasmid gives a five-fold increase in transformation of the qa2+ gene. Analysis of the DNA from Neurospora transformants revealed that the pMK2 plasmid is not stable. The qa insert as well as the LaBelle part of pMK2 are rapidly lost from the plasmid. In most cases the qa insert integrates into the nuclear DNA of the host. Plasmids recovered from Neurospora transformants are rearranged and show insertions or deletions. Some of these plasmids are described here. In most cases the qa insert and the LaBelle sequence of plasmid pMK2 have been deleted. Frequently plasmid dimers, carrying an insertion of mitochondrial DNA, are recovered.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Pediatric radiology 20 (1990), S. 447-450 
    ISSN: 1432-1998
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Five patients, whose espphageal atresia and distal tracheoesophageal fistula had been repaired in infancy, were examined by computed tomography at age 2 to 21 because of recurrent or persistent tracheal or esophageal problems. Their tracheas generally failed to have the roughly circular cross-sectional shape found in controls. The pars membranacea was often unusually broad, with much more section-to-section variation in area than in controls. Change in cross-sectional area with respiratory phase, in the one patient assessed, was much greater than in a control. These tracheal abnormalities help to explain the tracheal symptoms found in these and simular patients. Their esophagi contained much air and fluid.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5028
    Keywords: cDNA cloning ; light-harvesting chlorophyll a/b protein ; Pisum ; shoot-specific polypeptide ; small subunit ribulose 1,5 biphosphate carboxylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The molecular cloning of cDNA corresponds to pea seedling mRNA sequences encoding a shoot-specific polypeptide, the small subunit of the ribulose 1,5 biphosphate carboxylase and a component of the light-harvesting chlorophyll a/b complex is described. cDNA prepared from polysomal poly(A)RNA of light-grown shoots was enriched for shoot-specific and light-induced sequences by heterologous liquid hybridization with mercurated polysomal poly(A)RNA of dark-grown roots, followed by sulfhydryl chromatography. Cloned shoot-specific sequences were identified by 2D electrophoretic analysis of hybrid release translation products. The cloned shoot-specific sequence corresponded to a mRNA of 850 nt present both in light-and dark-grown shoots, and produced anin vitro translation product of Mr27 500 and isoelectric point of 4.7.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-2568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A plastic tissue adhesive, trifluoroisopropyl 2-cyanoacrylate (FlucrylateTM, MBR4197), was tested for hemostatic efficacy in acute laparotomy experiments using a canine model of acute bleeding gastric ulcer. An improved delivery system suitable for endoscopic use was developed. Hemostatic efficacy of the adhesive was tested in both briskly bleeding ulcers and in oozing ulcers after partial treatment with a heater probe. In pilot studies at laparotomy, primary and adjunctive cyanoacrylate therapy of 81 bleeding ulcers were evaluated in seven unheparinized foxhounds. Hemostasis was produced in 11% of ulcers treated with cyanoacrylate alone and in 31% of ulcers treated with cyanoacrylate as an adjunctive after partial heater-probe treatment; no sham-treated control ulcers stopped bleeding under the conditions of the experiment. To evaluate FlucrylateTM using our standard heparinized ulcer model, a randomized study was performed in six heparinized foxhounds at laparotomy. Ulcers were randomized to treatment with cyanoacrylate alone, adjunctive cyanoacrylate, heater probe alone or untreated control. Sham-treated control ulcers or ulcers treated with cyanoacrylate alone did not stop bleeding; 42% of ulcers treated with cyanoacrylate as an adjunctive stopped bleeding; all ulcers treated with a heater probe stopped bleeding. In this experimental model of acute bleeding gastric ulcer, trifluoroisopropyl 2-cyanoacrylate (FlucrylateTM, MBR4197) did not stop severe bleeding and was unpredictable as an adjunctive treatment.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1617-4623
    Keywords: Neurospora ; Transformation ; Mitoribosomal protein gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We showed previously that the Neurospora crassa mutation cyt-21-1 causes abnormal splicing of cyt-21 pre-mRNA, putatively leading to an internally deleted mitochondrial ribosomal protein. In this study, deletion derivatives of the Neurospora crassa cyt-21 + gene are used to complement cyt-21-1. Large parts of the 3′ end of the gene, comprising almost the entire last exon, can be deleted without affecting the transformation frequency. Analysis of DNA from transformants shows that the transforming DNA integrates non-specifically into the genome of the host. Northern analysis showed that the 3′ end of the cyt-21 + gene can be altered dramatically without affecting the 3′ processing of transcripts. One deletion derivative, in which part of the coding region of the gene is removed, yields transformants expressing a carboxyterminally modified ribosomal protein. The direct relationship between in vitro modification of the cloned Neurospora cyt-21 + gene and the detection of a modified mitoribosomal protein in cyt-21 transformants provides firm evidence that the cyt-21 + gene encodes a mitoribosomal protein.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 20 (1982), S. 351-356 
    ISSN: 1741-0444
    Keywords: Carotid artery ; Doppler ultrasound ; Feature extraction ; Principal component factor analysis ; Transient cerebral ischaemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Principal component factor analysis, a mathematical feature extraction technique, has been used to analyse the total information contained in the Doppler signal. In this study two patient groups have been investigated, normals and stenoses of less than 50%. The patients have been classified according to angiographic findings (patients with hypertension, migrane, heart disease, etc. havenot been excluded). The results from the principal component analysis technique have been compared with the more familiar A/B ratio based on the maximum frequency enevelope. Of the 25 normal vessel segments 20 were classified as normal by the A/B ratio technique and 22 by the principal component technique, while of the 19 abnormal vessels 13 were classified as abnormal by the A/B technique and 17 by the principal component analysis. Also the principal component analysis of the total Doppler signal was statistically superior to the A/B ratio in separating the two groups examined in this study.
    Type of Medium: Electronic Resource
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