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  • 1
    Electronic Resource
    Electronic Resource
    028
Early ProInflammatory Cytokine Expression During Mucosal Wound Healing (2005)
    Oxford, UK; Malden, USA : Blackwell Publishing Ltd/Inc.
    Wound repair and regeneration 13 (2005), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Proinflammatory cytokines (such as IL-1 [alpha], IL-1 [beta]) have been shown to play a significant role in early stages of wound healing. Previous studies also indicate that mucosal wounds heal faster in males. The purpose of this study was to assess gene expression of inflammatory cytokines before and during wound healing. Younger and older volunteers received two small oral wounds on the hard palate. One of the wounds (3.5 mm in diameter) was videographed daily until healed. The other wound (1 × 5 mm) was biopsied either 6 or 24 h postwounding. Real-time PCR (ABI Prism 7000 SDS) was performed on the unwounded and wounded tissues to study gene expression. Preliminary analyses revealed that compared to unwounded tissue, wounding induced rapid gene expression for these cytokines. In males, IL-1 [beta] gene expression peaked at 6 h and was higher than in females. Since males have been shown to heal this type of wound faster than females, this earlier peak of IL-1 [beta] may be beneficial to wound healing. Regardless of gender, IL-1ra and IL-1 [alpha] levels peaked at 6 and 24 h, respectively. Interestingly, cytokine levels in unwounded tissue correlated with wound healing parameters (e.g., wound sizes, time to heal), but in females only. Specifically, women with higher basal levels of IL-1 [beta] and IL-1ra, and lower levels of IL-1 [alpha] tended to heal faster. Thus, the expression of inflammatory cytokine genes in unwounded mucosal tissue may play a determinant role in wound healing outcomes.(Supported by NIH P50 DE-13749)
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2005.130215ab.x
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  • 2
    Electronic Resource
    Electronic Resource
    020
Stress-Induced Modulation of Matrix Metalloproteinases During Cutaneous Wound Repair (2005)
    Oxford, UK; Malden, USA : Blackwell Publishing Ltd/Inc.
    Wound repair and regeneration 13 (2005), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Chronic nonhealing wounds have been long associated with an imbalance between the degrading metalloproteinases (MMPs) and their inhibitors (TIMPs). Human and animal studies have shown psychological stress to impair healing and differentially regulate gene expression. We hypothesized that psychological stress-impaired healing is mediated by altered expression of MMP and TIMP. SKH-1 mice were subjected to restraint stress 3 days before and 5 days after placement of 3.5 mm biopsy wounds. The wounds were biopsied at various time points and analyzed for gene expression by real-time PCR. The wounds of stressed mice showed significantly delayed healing when compared to the wounds of controls through day 5 postwounding. There was a significantly higher expression of MMP-8 in the stressed groups on days 1 (172%, p 〈 0.0003) and 3 (204%, p 〈 0.0003) postwounding. Stress decreased MMP-9 expression on day 5 postwounding (56%, p 〈 0.02), but showed no significant difference to controls on the other days. TIMP-1, which is known to inhibit most activated MMPs, had significantly lower expression on day 5 postwounding (56%, p 〈 0.0003). TIMP-2, which is constitutively expressed, did not show any difference in expression between the stress and the control groups. During stress, increased MMP-8 (neutrophil collagenase) activity is in agreement with previous studies in our model that have demonstrated a increase in neutrophil recruitment on days 3 and 5 postwounding. Likewise, lower expression of TIMP-1 during stress is similar to findings in chronic wounds as opposed to healing surgical wounds. Hence, altered expression of the metalloproteinases and their inhibitors, may lead to increased damage early in healing and decreased repair later in stress-impaired wound healing.Supported by NIDCR-P50(DE13749), NIA-P01(AG16321)
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2005.130215t.x
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  • 3
    Electronic Resource
    Electronic Resource
    164
Amygdala Lesions Increase IL-8 and MIP-1[alpha] Gene Expression in Dermal Wound Tissue (2005)
    Oxford, UK; Malden, USA : Blackwell Publishing Ltd/Inc.
    Wound repair and regeneration 13 (2005), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Cortisol has been shown to impact upon wound healing through its immunosuppressive properties, and its release is modulated by the central nucleus of the amygdala (CeA) which is involved in fear and stress reactions. This study assessed the role of the CeA in modulating proinflammatory chemokine expression following wounding, under both stress and nonstress conditions. Bilaterally CeA-lesioned rhesus monkeys and nonoperated controls received separate dermal wounds on 3 consecutive days. Six hours postwounding, tissue biopsies were obtained from each wound. Following this, animals were subjected to 2 h of confinement as a stressor. Thus, examination of tissues on day 1 represented a nonstressed condition, and on days 2–3 a stressed condition. Using real-time PCR, we determined mRNA expression for IL-8 and MIP-1[alpha] in wounded and unwounded tissue, two chemokines critical in the recruitment and activation of neutrophils and monocytes in response to tissue injury. Analyses revealed that tissue mRNA levels for IL-8 and MIP-1[alpha] were increased in both groups following wounding. However, these rises were significantly greater in CeA-lesioned animals than controls. Repeated stress (confinement) attenuated these increases in mRNA expression equally in both groups (days 2–3). These results indicate that the inflammatory response to skin wounds is modulated, in part, by the amygdala. However, stress-induced alterations in inflammation are not CeA dependent for this particular stressor. Importantly, this finding indicates that a lesion of a central brain structure can impact upon peripheral wound healing parameters.(Supported by P50 DE-13749, MH46729, MH52354, MH61083)
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2005.130216bp.x
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  • 4
    Electronic Resource
    Electronic Resource
    153
Depressed and Lonely Individuals Express Lower EGF and VEGF in Saliva (2005)
    Oxford, UK; Malden, USA : Blackwell Publishing Ltd/Inc.
    Wound repair and regeneration 13 (2005), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) are present in saliva and may impact wound healing within the oral cavity. Previous studies in our lab have demonstrated that psychosocial factors such as elevated depression scores are associated with delayed oral wound healing. The aim of this study was to determine the relationship between salivary levels of EGF and VEGF and depression and loneliness scores. Whole saliva was collected from 43 individuals in groups aged 18–35 years and 50+ years. On the same day, volunteers were asked to complete the Beck’s Depression Inventory (BDI) and the UCLA loneliness scale. Salivary concentrations of EGF and VEGF were determined by standard ELISA. Mean concentrations of EGF and VEGF were 2860 pg/ml and 421 pg/ml, respectively. In the younger age group, significant negative correlations were found between scores on the BDI and UCLA indices and salivary levels of EGF. A median split, based upon BDI scores, similarly revealed that more depressed or lonely individuals exhibited significantly lower EGF levels. A similar trend was observed for VEGF. These preliminary results indicate that psychosocial factors such as depression and loneliness may be related to growth factor expression in saliva. This, in turn, may play a part in the delayed mucosal wound healing observed in depressed individuals.(Supported by NIH P50 DE-13749)
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2005.130216be.x
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  • 5
    Electronic Resource
    Electronic Resource
    An in vitro model of cell migration: Evaluation of vascular endothelial cell migration
    Amsterdam : Elsevier
    Analytical Biochemistry 190 (1990), S. 39-47 
    Details
    ISSN: 0003-2697
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0003-2697(90)90130-2
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