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  • 1
    Electronic Resource
    Electronic Resource
    An embryogenic cell line of maize from A188 (Minnesota) contains Mu1-like elements (1988)
    Springer
    Plant molecular biology 10 (1988), S. 273-279 
    Details
    ISSN: 1573-5028
    Keywords: embryogenic cell culture ; maize ; Mutator ; transposable elements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The maize inbred line A188 is popularly used for the production of embryogenic cell lines. A188, maintained at the University of Minnesota, was found upon molecular analysis to contain 2 to 4 copies of a DNA sequence very similar in structure to transposable Mu1 elements, which have been implicated in Robertson's Mutator system. These Mu1-like elements are in the same chromosomal locations in sibling plants and in A188 cell cultures derived from them. This suggests that the elements are in an inactive state and do not undergo transposition. However, we have observed that they are not modified at the target sites for certain restriction endonucleases. Possible causes for the apparent lack of transposition of these Mu1-like elements in these A188 lines are discussed. Inasmuch as the elements do not transpose, they must be maintained in this line as homozygous Mendelian elements by self-pollination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027404
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A maize Ds transposable element containing a dihydrofolate reductase gene transposes in Nicotiana tabacum and Arabidopsis thaliana (1989)
    Springer
    Molecular genetics and genomics 219 (1989), S. 461-466 
    Details
    ISSN: 1617-4623
    Keywords: Transposition ; Gene tagging ; Dihydrofolate reductase ; Arabidopsis thaliana ; Activator
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A mouse dihydrofolate reductase gene (DHFR), encoding an enzyme conferring methotrexate (MTX) resistance, under the control of the cauliflower mosaic virus (CaMV) 35 S promoter, was inserted within a maize nonautonomous Ds transposable element. The presence of at least one element (Ds-DHFR) can easily be monitored using methotrexate selection in plants. This chimeric element is able to transpose at a frequency similar to its unmodified progenitor in transgenic tobacco callus containing an autonomous Ac element. The orientation of the selectable marker cassette in the Ds element does not affect relative excision frequencies. Approximately two-thirds of these elements can be detected after excision while the remaining one-third cannot. The Ds-DHFR element is useful in elucidating the mechanism by which Ac/Ds transposition occurs, and allows for a rapid identification of mutants in which methotrexate resistance cosegregates with a mutant phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00259620
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    Paper (German National Licenses)
    Fulltext
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