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  • 1
    Electronic Resource
    Electronic Resource
    Participation of poly(ADP-robose) polymerase in the drug sensitivity in human lung cancer cell lines (1992)
    Springer
    Journal of cancer research and clinical oncology 118 (1992), S. 244-248 
    Details
    ISSN: 1432-1335
    Keywords: Poly(ADP-ribose) polymerase ; Drug resistance ; Lung cancer cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Poly(ADP-ribose) polymerase has been generally assumed to be involved in DNA repair. The level of the enzyme in various lung cancer cell lines was examined to determine if it is involved in drug resistance. Among nine cell lines of lung cancer tested, small-cell lung cancer lines, which showed higher sensitivity to cisplatin and etoposide, were unexpectedly found to contain significantly higher poly(ADP-ribose) polymerase activity than five non-small-cell lung cancer cell lines. This activity inversely correlated with IC50 values of lung cancer cell lines to etoposide, an inhibitor of topoisomerase II. The polymerase activity was also examined in several cisplatin-resistant variants of the cell lines. However, no difference was observed between parental and cisplatin-resistant cells. There was no significant relation between poly(ADP-ribose) polymerase activity and IC50 values for cisplatin and carboplatin. Although this enzyme was considered to play some role in the resistance to specific drugs, it might not be a critical factor in cisplatin-induced cytotoxicity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01208612
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  • 2
    Electronic Resource
    Electronic Resource
    Function of poly(ADP-ribose) polymerase in response to DNA damage: Gene-disruption study in mice (1999)
    Springer
    Molecular and cellular biochemistry 193 (1999), S. 149-152 
    Details
    ISSN: 1573-4919
    Keywords: poly(ADP-ribose) polymerase ; gene-disruption ; embryonic stem cell ; DNA damage responses ; p53 ; pRB ; cdk2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract To elucidate the biological functions of poly(ADP-ribose) polymerase (PARP, [EC 2.4.2.30]) in DNA damage responses, genetic and biochemical approaches were undertaken. By disrupting exon 1 of the mouse PARP gene by a homologous recombination, PARP-deficient mouse embryonic stem (ES) cell lines and mice could be produced without demonstrating lethality. PARP-/- ES cells showed complete loss of PARP activity and increased sensitivity to γ-irradiation and an alkylating agents, indicating a physiological role for PARP in the response to DNA damage. p53, a key molecule in cellular DNA damage response, was found to stimulate PARP activity and became poly(ADP-ribosyl)ated in the presence of damaged DNA. However, PARP-/- ES cells showed p21 and Mdm-2 mRNA induction following γ-irradiation, indicating that PARP activity is not indispensable for p21 and Mdm-2 mRNA induction in the established p53-cascade. On the other hand, in a reconstituted reaction system, purified PARP from human placenta suppressed the pRB-phosphorylation activity in the presence of NAD and damaged DNA. Human PARP expressed in E. coli showed a similar effect on pRB-phosphorylation activity of cdk2. These findings suggest a direct involvement of PARP in the regulation of cdk activity for cell-cycle arrest.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006941016799
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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