ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
Human tau-protein kinase I (TPK-I; also known as glycogen synthase kinase-3β, GSK-3β) is a serine/threonine protein kinase. Full-length TPK-I/GSK-3β was expressed in Escherichia coli as a fusion protein with a 6×His tag at the C-terminus and was crystallized using the hanging-drop vapour-diffusion method. Prismatic crystals of dimensions 0.4 × 0.2 × 0.1 mm were obtained using 12–15%(w/v) polyethylene glycol 6000 as a precipitant at 278 K. The crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 82.9, b = 86.1, c = 178.1 Å measured at 100 K, diffract to 2.3 Å resolution and seem to contain two enzyme molecules per asymmetric unit.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444900010386
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