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  • 1
    Electronic Resource
    Electronic Resource
    Copia-like retrotransposons in rice: sequence heterogeneity, species distribution and chromosomal locations (1997)
    Springer
    Plant molecular biology 33 (1997), S. 1051-1058 
    Details
    ISSN: 1573-5028
    Keywords: genetic diversity ; indica ; japonica ; mapping ; reverse transcriptase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Degenerated oligonucleotide primers were used to amplify, clone, and analyze sequence heterogeneity and chromo-somal distribution of 23 PCR fragments corresponding to the reverse transcriptase domain of copia-like retrotrans-posons in rice. Of the 23 fragments 22 could be aligned by their deduced amino acid sequences and were divided into 6 groups according to the phylogenetic and Southern blot analyses. Amino acid sequence differences among the 22 aligned fragments ranged from 1 to 64%. Southern blot analysis of 10 rice accessions including indica, japonica and common wild rice, using these 23 fragments as probes, showed that copia-like retrotransposons were present in moderate to high copy numbers in all the rice genome although the exact copy number cannot be determined. The major difference revealed by southern analysis is a differentiation between the four indica varieties as one group and the four japonica varieties and the two wild rice accessions as another group. Polymorphisms were also detected among the indica and japonica varieties by major bands and repeatable minor bands. Five hybridization bands were mapped to chromosomes 3, 4, 8, and 9, respectively. All the five bands were inherited in a dominant Mendelian fashion and were not allelic with each other, indicating that the same element did not reside on the same location in different rice accessions. No transcript of the copia-like reverse transcriptase was detected on northern blot. The results suggest that the sequence heterogeneity and distributional variability of retrotransposons may be one of contributory factors causing genetic diversity in rice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005715118851
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of quantitative trait loci controlling sucrose content in soybean (Glycine max) (2000)
    Springer
    Molecular breeding 6 (2000), S. 105-111 
    Details
    ISSN: 1572-9788
    Keywords: carbohydrates ; genetic mapping ; oil ; protein ; QTL ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Sucrose is a primary constituent of soybean (Glycine max) seed; however, little information concerning the inheritance of seed sucrose in soybean is available. The objective of this research was to use molecular markers to identify genomic regions significantly associated with quantitative trait loci (QTL) controlling sucrose content in a segregating F2 population. DNA samples from 149 F2 individuals were analyzed with 178 polymorphic genetic markers, including RFLPs, SSRs, and RAPDs. Sucrose content was measured on seed harvested from each of 149 F2:3 lines from replicated field experiments in 1993 and 1995. Seventeen marker loci, mapping to seven different genomic regions, were significantly associated with sucrose variation at P〈0.01. Individually, these markers explained from 6.1% to 12.4% of the total phenotypic variation for sucrose content in this population. In a combined analysis these genomic regions; explained 53% of total variation for sucrose content. No significant evidence of epistasis among QTLs was observed. Comparison of our QTL mapping results for sucrose content and those previously reported for protein and oil content (the other major seed constituents in soybean), suggests that seed quality traits are inherited as clusters of linked loci or that `major' QTLs with pleiotropic effects may control all three traits. Of the seven genomic regions having significant effects on sucrose content, three were associated with significant variation for protein content and three were significantly associated with oil content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009628614988
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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