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  • 1
    Electronic Resource
    Electronic Resource
    Reduction in TrkA-Immunoreactive Neurons Is Not Associated with an Overexpression of Galaninergic Fibers Within the Nucleus Basalis in Down's Syndrome (2000)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 74 (2000), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Down's syndrome (DS) individuals develop neuropathological features similar to Alzheimer's disease (AD), including degeneration of cholinergic basal forebrain (CBF) neurons. In AD a reduction in CBF/trkA-containing neurons has been suggested to trigger a hyperexpression of galaninergic fibers within the nucleus basalis subfield of the basal forebrain. The present study examined the interrelationship between reductions in CBF/trkA-containing neurons and the overexpression of galaninergic fibers within the nucleus basalis in DS. Within the nucleus basalis stereologic evaluation revealed a 46% reduction in the number of trkA-immunopositive neurons, whereas optical density measurements displayed a nonsignificant 18% reduction in neuronal trkA immunoreactivity in DS as compared with age-matched controls. Western blot analysis also showed a significant reduction in cortical trkA protein levels in DS. A semiquantitative examination of galaninergic fibers in the nucleus basalis revealed only a modest hypertrophy of galaninergic fibers within the nucleus basalis in DS. The present findings indicate a significant reduction in trkA within the nucleus basalis and cortex with only a moderate hypertrophy of galaninergic fibers in DS. These observations suggest that DS may not be an exact genetic model for investigation of changes in the AD basal forebrain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.2000.741185.x
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  • 2
    Electronic Resource
    Electronic Resource
    Proinflammatory cytokines promote glial heme oxygenase-1 expression and mitochondrial iron deposition: implications for multiple sclerosis (2001)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 77 (2001), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Proinflammatory cytokines, pathological iron deposition, and oxidative stress have been implicated in the pathogenesis of multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE). HO-1 mRNA levels and mitochondrial uptake of [55Fe]Cl3-derived iron were measured in rat astroglial cultures exposed to interleukin-1β (IL-1β) or tumor necrosis factor-α (TNF-α) alone or in combination with the heme oxygenase-1 (HO-1) inhibitors, tin mesoporphyrin (SnMP) or dexamthasone (DEX), or interferon β1b (INF-β). HO-1 expression in astrocytes was evaluated by immunohistochemical staining of spinal cord tissue derived from MS and control subjects. IL-1β or TNF-α promoted sequestration of non-transferrin-derived 55Fe by astroglial mitochondria. HO-1 inhibitors, mitochondrial permeability transition pore (MTP) blockers and antioxidants significantly attenuated cytokine-related mitochondrial iron sequestration in these cells. IFN-β decreased HO-1 expression and mitochondrial iron sequestration in IL-1β- and TNF-α-challenged astroglia. The percentage of astrocytes coexpressing HO-1 in affected spinal cord from MS patients (57.3% ± 12.8%) was significantly greater (p 〈 0.05) than in normal spinal cord derived from controls subjects (15.4% ± 8.4%). HO-1 is over-expressed in MS spinal cord astroglia and may promote mitochondrial iron deposition in MS plaques. In MS, IFN-β may attenuate glial HO-1 gene induction and aberrant mitochondrial iron deposition accruing from exposure to proinflammatory cytokines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.2001.00354.x
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  • 3
    Electronic Resource
    Electronic Resource
    Novel application of quantitative immunoassays for screening seed globulins of cowpea varieties (1985)
    Springer
    Bioscience reports 5 (1985), S. 673-681 
    Details
    ISSN: 1573-4935
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Using antibodies raised in rabbits, radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) are standardized for cowpea (var. Pusa Barsati) seed globulins. The RIA, when used to screen three stages of seed development, reveals that maximum globulins are detected at 28 days after flowering. When three different varieties of cowpea are assayed for their globulin content by RIA and ELISA, it is observed that the Bold Grain cowpea has the highest amount of related globulin as compared to two other varieties, namely Pusa Phalgun and Asparagus Bean.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01116999
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  • 4
    Electronic Resource
    Electronic Resource
    A sensitive and rapid immunochemical method for quantitation of proteins (1988)
    Springer
    Bioscience reports 8 (1988), S. 427-433 
    Details
    ISSN: 1573-4935
    Keywords: ELISA ; storage proteins ; globulins ; pigeon pea (Cajanus cajan)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A sensitive and rapid ELISA for quantitation of seed globulins is described. This method employs conjugation of pigeon pea (Cajanus cajan) globulin antibodies and the enzyme peroxidase together with dextran. Using this conjugate, proteins as low as 0.1 ng were detected. Dextran conjugate has a ten-fold greater efficiency of quantitating pigeon pea globulins than the commercial goat anti-rabbit IgG conjugate, and is three-fold more efficient than pigeon pea globulin IgG peroxidase conjugate. The method can be conveniently adapted for quantitation of other proteins also.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01121640
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  • 5
    Electronic Resource
    Electronic Resource
    Export of proteins across membranes: The helix reversion hypothesis (1989)
    Springer
    Bioscience reports 9 (1989), S. 737-745 
    Details
    ISSN: 1573-4935
    Keywords: protein export ; conformational changes ; leader peptides ; hairpin helix
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A model is presented which explains the biological role of the leader peptide in protein export. Along the lines of this model, the conformational changes of a protein with environment serves as a general mechanism for translocation. The leader peptide in the cytoplasm takes a hairpin like conformation which reverts to an extended helix upon integration into the membrane. The essential features of this model are in accord with recent results of protein export.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01114812
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  • 6
    Electronic Resource
    Electronic Resource
    Silicalite zeolite : A novel matrix for purification and estimation of a hydrophobic protein rice prolamin (1990)
    Springer
    Biotechnology techniques 4 (1990), S. 177-180 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A simple and easy affinity purification of antibodies raised against a highly hydrophobic protein is described. This method employs silicalite zeolite, a solid matrix, to which prolamin is adsorbed. A rapid and sensitive immunoradiometric assay was developed to quantitate prolamin. Maximum prolamin in rice is present between 10 to 14 days post-anthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222501
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  • 7
    Electronic Resource
    Electronic Resource
    Quantitation of rice glutelin, an acid/alkali/urea soluble protein, by enzyme linked immunosorbent assay (1990)
    Springer
    Biotechnology techniques 4 (1990), S. 311-314 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A sensitive and rapid ELISA for quantitation of rice glutelin was developed. This method employed binding of rice glutelin to Immulon I microtitre plate and quantitating it with commercial goat anti-rabbit IgG conjugate by conventional ELISA. Using this procedure, glutelin as low as 5ng could be detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00157427
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