ISSN:
1573-5044
Keywords:
transient gene expression
;
β-glucuronidase
;
alfalfa
;
soybean
;
DNA transfer
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A simple particle bombardment device was designed, constructed and shown to be efficient for the delivery of DNA into plant cells. High levels of transient β-glucuronidase expression were observed in alfalfa suspension-cultured cells and embryogenic soybean suspension-cultured cells. Expression of β-glucuronidase in alfalfa suspension-cultured cells was used to optimize the bombardment conditions for the device. Transient gene expression in alfalfa was found to be dependent on the state of the target tissue, the size of particles employed, the helium pressure used to accelerate the particles and the distance travel led by the tungsten particles carrying DNA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00048116
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