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  • 1
    Electronic Resource
    Electronic Resource
    Transcription and Motoneuron Size (1994)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 63 (1994), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Nuclear size and total RNA synthesis were compared in single lumbar motoneurons isolated from the grass frog. Transcription was found to correlate significantly, but not exclusively, with nuclear area or volume over a wide range of nuclear size, the largest nuclei having the highest mean transcriptional activity. Flow cytometric analysis of propidium iodide-stained nuclei excluded polyploidy or polyteny as an explanation for the increased transcription, but left open the possibility of a small increase in DNA with increasing nuclear size. Alternatively, motoneurons may increase transcription and nuclear size without increasing their DNA content, possibly by increasing the proportion of dispersed chromatin (euchromatin). These two mechanisms for size-related changes in RNA synthesis in motoneurons present an interesting contrast to mechanisms used by many other large animal cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.1994.63051609.x
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  • 2
    Electronic Resource
    Electronic Resource
    Distribution of Soluble Proteins Within Spinal Motoneurons: A Quantitative Two-Dimensional Electrophoretic Analysis (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 36 (1981), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Soluble protein fractions obtained from bovine lumbar spinal motoneuron cell bodies, ventral gray matter, and ventral and dorsal roots were analyzed by two-dimensional gel electrophoresis. Each extract was separated into Coomassie blue-stained patterns of up to 350 polypeptides ranging in isoelectric point from pH 4 to 8 and in molecular weight from 10,000 to 200,000. Visual inspection of the protein pattern of the isolated cell bodies showed it to be substantially different from those of ventral gray matter and the spinal roots, while the patterns obtained from ventral and dorsal roots were indistinguishable. Computer-assisted densitometry of the major soluble proteins from spinal roots showed no quantitative difference between the predominant proteins in ventral and dorsal root extracts. Differences of 10-fold or more were common when the major proteins of the isolated perikarya were compared with those of the other fractions. Since most of the soluble proteins extracted from ventral and dorsal roots were probably derived from the axoplasm of motor and sensory nerves, respectively, these results are interpreted to mean that large differences exist in the distribution of individual soluble proteins between the cell body and axon of spinal motoneurons, while the major soluble proteins of spinal motor and sensory axons are highly similar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1981.tb02400.x
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  • 3
    Electronic Resource
    Electronic Resource
    CHOLINE ACETYLTRANSFERASE ACTIVITY IN LARGE VENTRAL SPINAL NEURONS (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 29 (1977), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— Up to approx 3 pmol of acetylcholine (ACh)/h/cell body was synthesized by perikarya of large spinal neurons isolated in bulk fractions from bovine ventral spinal cord. Many of the cell bodies are probably derived from motoneurons. A medium of low ionic strength and pH was used to minimize losses of soluble acetyl CoA:choline-o-acetyltransferase (ChAc; EC 2.3.1.6) from the neurons, whose permeability properties were altered. Such a medium also increased the retention of other soluble proteins by the cell bodies. The maximal rate of hydrolysis of ACh by the isolated neurons exceeded that of its synthesis by a factor of at least 100. It was estimated that ChAc and acetylcholinesterase (AChE; EC 3.1.1.7) each represent less than 0.01% by weight of the total protein in these cell bodies and that as little as 10% of each enzyme in the ventral spinal cord is located within the large neuronal somata and their proximal processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1977.tb10727.x
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  • 4
    Electronic Resource
    Electronic Resource
    MEMBRANE FRAGMENTS FROM MYELIN TREATED WITH DIFFERENT DETERGENTS (1971)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 18 (1971), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Bovine CNS myelin treated with cationic, anionic and non-ionic detergents yielded fragments which did not sediment in water at 56,500 g for 15 min. Substantial chemical and immunological differences were observed among the myelin fragments produced by these detergents. The chemical and immunological characteristics of such membrane products were also dependent upon the concentration of detergent. These and similar findings with other biological membranes emphasize the difficulties in using detergents to obtain and examine proposed subunit structures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1971.tb00182.x
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  • 5
    Electronic Resource
    Electronic Resource
    THE NUCLEAR DNA CONTENT OF LARGE VENTRAL SPINAL NEURONS (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 27 (1976), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— A nuclear fraction has been obtained from large spinal neurons previously isolated from bovine ventral spinal cord in bulk suspensions. The fraction contained an average of 5.3 ± 0.9 pg DNA/nucleus, indicating a high incidence of diploid nuclei. This conclusion was confirmed by distribution analysis of DNA in propidium iodide-stained nuclei examined by flow microfluorometry. That technique showed that at least 90% of the nuclei from large spinal neurons are diploid. Mixed, mostly non-neuronal nuclei derived from many types of cells in the ventral spinal cord contained an average of 5.9 ± 0.6 pg DNA/nucleus, 19% of which possibly possess more than diploid amounts of DNA. The uniform DNA content in nuclei of large spinal neurons and most other types of cells in the ventral spinal cord contrasts sharply with a wide variation (av 26-fold) in the nuclear volumes of the same cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1976.tb01551.x
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  • 6
    Electronic Resource
    Electronic Resource
    BULK ISOLATION OF LARGE VENTRAL SPINAL NEURONS (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 25 (1975), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: —Cell bodies of spinal neurons can be isolated in bulk fractions, using methods developed for neurons from brain. The technique described here includes sieving steps which allow one to purify and concentrate selectively the larger neuronal perikarya (50 μm or more), most of which could be alpha motoneurons. An average of 170,000 large neurons can be obtained from 50 g of trimmed bovine ventral enlargements. Roughly 6% of the total large neurons in the ventral grey matter are recovered, and 85% of the isolated neurons are larger than 50 μm in their smallest dia. Non-neuronal contaminants, mostly capillary fragments, account for 19% of the total particles in the fraction. Each isolated neuron contains an average of 9690 pg protein and 1353 pg RNA and hydrolyses 2·2 pmol of acetylthiocholine/min. Pretreatment of the spinal tissue with collagenase before cell isolation is also evaluated here.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1975.tb04358.x
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  • 7
    Electronic Resource
    Electronic Resource
    Characterization of the Phospholipids in Pinus ponderosa Pollen* (1966)
    s.l. : American Chemical Society
    Biochemistry 5 (1966), S. 4054-4061 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00876a040
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