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  • 1
    Digitale Medien
    Digitale Medien
    Verification of the introgression of Erianthus arundinaceus germplasm into sugarcane using molecular markers (2005)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 124 (2005), S. 0 
    Details
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Erianthus arundinaceus has a number of important agronomic traits including good ratooning ability, tolerance to both drought and water logging, disease resistance and vigour and is of interest as a potential source of parental germplasm to sugarcane breeders. However, to date, attempts to produce fertile hybrids between sugarcane (Saccharum spp.) and E. arundinaceus have been unsuccessful. Microsatellite markers that generated genus-specific markers and were highly polymorphic within sugarcane were identified. The microsatellite markers and 5S rDNA PCR were used to screen intergeneric (F1) clones from Saccharum officinarum×E. arundinaceus crosses, and two Saccharum backcross progeny (BC1) populations derived from crosses between selected F1 clones and sugarcane (Saccharum spp.), to identify genuine Saccharum spp. BC1 progeny. The 5S rDNA PCR marker and highly polymorphic microsatellites with Erianthus-specific bands confirmed the F1 parent of the two putative BC1 populations was a S. officinarum×E. arundinaceus hybrid and allowed the identification of the genuine BC1 progeny from selfs of the F1 parent. This is the first verification of BC1 progeny from an F1 intergeneric hybrid x sugarcane (Saccharum spp.) clone with molecular markers and confirms the introgression of E. arundinaceus germplasm into sugarcane. It should now be possible to exploit genes of value from E. arundinaceus in sugarcane breeding programmes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1439-0523.2005.01099.x
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  • 2
    Digitale Medien
    Digitale Medien
    Relationships between hard-seededness and seed weight in mungbean (Vigna radiata) assessed by QTL analysis (2005)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 124 (2005), S. 0 
    Details
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Weather damage reduces the value of commercial mungbean. but hard-seededness can reduce the level of damage. However. attempts lo breed large- and hard-seeded mungbean varieties have been unsuccessful. To understand the relationship between seed weight and hard-seededness. these trails were investigated using a quantitative trail loci (QTL) mapping approach with a recombinant inbred population derived from a cross between a completely soft-seeded variety and a completely hard-seeded genotype. The two parental genotypes also had a sixfold difference in seed weight. QTL analyses revealed four loci for hard-seededness and 11 loci for seed weight. Two of the hard-seeded ness loci co-localized with seed weight QTL. When seed weight was used as a covariate in the analysis of hard-seededness from the field data, two of the four hard-seeded QTL remained significant with the effect al one of these remaining unchanged. These results explain why retaining hard-seededness in large seeded mungbean lines has been unsuccessful. The existence of a persistent locus, however. indicated that breeding large and persistently hard-seeded varieties of mungbean may be possible.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1439-0523.2005.01084.x
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  • 3
    Digitale Medien
    Digitale Medien
    Identification of genomic regions associated with stay green in sorghum by testing RILs in multiple environments (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 1225-1232 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Sorghum ; Genome mapping ; Stay green ; Marker-assisted selection ; Multi-environment testing
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Stay green is an important drought resistance trait for sorghum production. QTLs for this trait with consistent effects across a set of environments would increase the efficiency of selection because of its relatively low heritability. One hundred and sixty recombinant inbreds, derived from a cross between QL39 and QL41, were used as a segregating population for genome mapping and stay green evaluation. Phenotypic data were collected in replicated field trials from five sites and in three growing seasons, and analysed by fitting appropriate models to account for spatial variability and to describe the genotype by environment interaction. Interval mapping and non-parametric mapping identified three regions, each in a separate linkage group, associated with stay green in more than one trial, and two regions in single trial. The regions on linkage groups B and I were both consistently identified from three trials. The multiple environment testing was very helpful for correctly identifying QTLs associated with the trait. The utilisation of molecular markers for stay green in sorghum breeding is also discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051428
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  • 4
    Digitale Medien
    Digitale Medien
    Analysis of phylogenetic relationships in the Triticeae tribe using RFLPs (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 649-655 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Cladogram ; Phenogram ; Phylogenetic tree ; Restriction fragment length polymorphisms ; Triticeae tribe
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The use of restriction fragment length polymorphisms in combination with other approaches is very useful for the reconstruction of evolutionary events revealing phylogenetic relationships. A set of 21 cDNA probes hybridizing to different chromosome arms in hexaploid wheat was used in a series of experiments designed to estimate the phylogenetic relationships among and within 16 species of the Triticeae tribe. A high degree of polymorphism was found both between and within the species examined. The RFLP data were used to generate a cladogram and a phenogram in order to compare the two different methods of constructing phylogenetic trees. The results of both methods were consistent with each other and with the general taxonomic information provided by earlier morphological studies, meiotic pairing analysis, isozyme tests, and sequence alignment in theTer,NOR, and5s DNA loci. In addition, several correlations were found between the geographical origin of accessions from the same species and their phylogenetic relationships as shown by the cladogram and phenogram.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00838722
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  • 5
    Digitale Medien
    Digitale Medien
    Ribosomal DNA variations in Erianthus, a wild sugarcane relative (Andropogoneae-Saccharinae) (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 733-743 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words rDNA ; 5S DNA ; RFLP ; Erianthus sect. Ripidium ; Saccharum complex ; Germplasm
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Variation at and 5S ribosomal DNA loci was assessed on 62 Erianthus Michx. clones, representing 11 species, and 15 clones from two Saccharum L. species used as a reference. Genus-specific markers for Erianthus Michx. sect. Ripidium Henrard (Old World species) were identified. Ribosomal DNA units in Erianthus sect. Ripidium exhibited an additional BamHI site compared to Saccharum, and 5S units showed length and restriction-site differences between Erianthus and Saccharum. These markers will be useful to follow introgression in SaccharumErianthus hybrids. Six ribosomal units (genes) were revealed in Erianthus sect. Ripidium, differing by restriction-site positions and/or length. These results provided new information on species relationships and evolution within the genus Erianthus. The Indonesian and Indian forms of E. arundinaceus (Retz.) Jeswiet gave different restriction patterns, which were similar to those of E. bengalense (Retz.) R. C. Bharadwaja and E. procerus (Roxb.) Raizade, respectively. The two species, E. ele-phantinus Hook.f. and E. ravennae (L.) P. Beauv., could also be differentiated at this locus. Two of the New World Erianthus species studied, E. rufipilus (Steud.) Griseb. and E. longisetosus Andersson, appeared more like Erianthus sect. Ripidium, whereas E. trinii Hack. and E. brevibardis Michx. showed patterns consistent with Miscanthus sinensis Andersson and S. spontaneum L., respectively. Finally, the comparison of rDNA restriction maps among Erianthus sect. Ripidium, Saccharum, sorghum and maize, led to unexpected conclusions concerning the relationships between the different genera and the position of Erianthus in the “Saccharum complex”.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050186
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  • 6
    Digitale Medien
    Digitale Medien
    Ribosomal DNA variations in Erianthus, a wild sugarcane relative (Andropogoneae-Saccharinae) (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 733-743 
    Details
    ISSN: 1432-2242
    Schlagwort(e): rDNA ; 5S DNA ; RFLP ; Erianthus sect. Ripidium ; Saccharum complex ; Germplasm
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Variation at the 18S+26S and 5S ribosomal DNA loci was assessed on 62 Erianthus Michx. clones, representing 11 species, and 15 clones from two Saccharum L. species used as a reference. Genus-specific markers for Erianthus Michx. sect. Ripidium Henrard (Old World species) were identified. Ribosomal DNA units in Erianthus sect. Ripidium exhibited an additional BamHI site compared to Saccharum, and 5S units showed length and restriction-site differences between Erianthus and Saccharum. These markers will be useful to follow introgression in Saccharum x Erianthus hybrids. Six ribosomal units (for 18+26S genes) were revealed in Erianthus sect. Ripidium, differing by restriction-site positions and/or length. These results provided new information on species relationships and evolution within the genus Erianthus. The Indonesian and Indian forms of E. arundinaceus (Retz.) Jeswiet gave different restriction patterns, which were similar to those of E. bengalense (Retz.) R. C. Bharadwaja and E. procerus (Roxb.) Raizade, respectively. The two 2n=20 species, E. ele-phantinus Hook.f. and E. ravennae (L.) P. Beauv., could also be differentiated at this locus. Two of the New World Erianthus species studied, E. rufipilus (Steud.) Griseb. and E. longisetosus Andersson, appeared more like Erianthus sect. Ripidium, whereas E. trinii Hack, and E. brevibardis Michx. showed patterns consistent with Miscanthus sinensis Andersson and S. spontaneum L., respectively. Finally, the comparison of rDNA restriction maps among Erianthus sect. Ripidium, Saccharum, sorghum and maize, led to unexpected conclusions concerning the relationships between the different genera and the position of Erianthus in the “Saccharum complex”.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226096
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  • 7
    Digitale Medien
    Digitale Medien
    Evolution and systematic relationships in theTriticeae (Poaceae) (1988)
    Springer
    Plant systematics and evolution 160 (1988), S. 1-28 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Poaceae ; Triticeae ; Systematics ; phylogeny ; cladistic ; phytogeography ; isozyme analyses ; chromosome pairing ; DNA analyses ; relationships ; molecular evolution
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The evolution and taxonomic relationships in theTriticeae are discussed with the view to highlight aspects of this agronomically important group of plants, which may be of interest to molecular biology. Some of these aspects are addressed in more detail in adjoining papers in which specific genomic loci have been examined at the DNA sequence or isozyme level. Aspects discussed include the systematics and geographic distribution of theTriticeae species, isozyme and chromosome pairing studies on some of the species as well as more recent developments in DNA analyses. A survey of the systematics of theTriticeae indicated that the genomic system ofLöve is probably the most useful starting point for interpreting molecular data even though the system has many problems from a taxonomic point of view. The geographical distribution ofTriticeae species, using both published and unpublished data, suggested that information of this type taken together with the theory of continental drift provides a broad time-span for considering data from DNA sequence studies. The significance, and modes of analyses, of isozyme studies were assessed because they often provide valuable characters in determining relationships between species. The main character underlyingLöve's andDewey's analyses of theTriticeae, namely chromosome pairing, is discussed with particular reference to isozyme studies to show that in some cases, such as species ofHordeum sensu lato, consistent relationships are obtained. Finally, new developments in understanding chromosome structure are considered in relation to the above variables in the taxonomy and evolution of theTriticeae.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00936706
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  • 8
    Digitale Medien
    Digitale Medien
    DNA sequence analyses of the ribosomal spacer regions in theTriticeae (1988)
    Springer
    Plant systematics and evolution 160 (1988), S. 91-104 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Poaceae ; Triticeae ; Ribosomal DNA spacer regions ; phenetic and molecular relationships
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Two regions of the ribosomal DNA (rDNA) were sequenced from a range of species from the tribeTriticeae. One region, the central spacer, was found to be more divergent in sequence than the other, the 18 S-spacer junction. Both regions contained sequences 20–30 bp long which were more highly conserved than the remainder of the region and their possible significance in rDNA expression is discussed. Phenetic relationships based on the sequence data were generally consistent with the relationships based on other criteria. Species possessing the S, E, J1J2, D, and B genomes clustered together, with the H genome species being the most distinct of those examined. The R, P, and V genome species occupy an intermediate position in the overall pattern of relationships. Some relationships differed in detail from those established by other parameters, for example the position of the N genome species, and explanations for discrepancies of this type are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00936712
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  • 9
    Digitale Medien
    Digitale Medien
    Relationships betweenOryza species (Poaceae) based on 5S DNA sequences (1992)
    Springer
    Plant systematics and evolution 183 (1992), S. 249-264 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Poaceae ; Oryza ; 5S DNA ; phylogeny ; evolution
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Relationships between 9Oryza species, covering 6 different genomes, have been studied using hybridization and nucleotide sequence information from the5S Dna locus. Four to five units of the major size class of 5S DNA in each species, 55 units in all, were cloned and sequenced. Both hybridization and sequence data confirmed the basic differences between the A and B, C, D genome species suggested by morphological and cytological data. The 5S DNA units of the A genome species were very similar, as were the ones from the B, C, and D genome-containing species. The 5S DNA ofO. australiensis (E genome) grouped with the B, C, D cluster, while the units ofO. brachyantha (F genome) were quite different and grouped away from all other species. 5S DNA units fromO. minuta, O. latifolia, O. australiensis, andO. brachyantha hybridized strongly, and preferentially, to the genomic DNA from which the units were isolated and hence could be useful as species/genome specific probes. The 5S DNA units fromO. sativa, O. nivara, andO. rufipogon provided A genome-specific probes as they hybridized preferentially to A genome DNA. The units fromO. punctata andO. officinalis displayed weaker preferential hybridization toO. punctata DNA, possibly reflecting their shared genome (C genome).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00940807
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  • 10
    Digitale Medien
    Digitale Medien
    Molecular analyses suggest a need for a significant rearrangement of Rutaceae subfamilies and a minor reassessment of species relationships withinFlindersia (2000)
    Springer
    Plant systematics and evolution 223 (2000), S. 15-27 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Rutaceae ; Rutoideae ; Citroideae ; Dictyolomatoideae ; Spathelioideae ; Flindersioideae ; Flindersia ; Molecular systematics ; phylogeny ; chloroplast ; trnL-trnF spacer ; ITS-1
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract DNA sequencing has been used to construct two molecular phylogenies at the intrafamily and intrageneric level within the Rutaceae. Analysis oftrnL-trnF sequence data for five Rutaceae subfamilies has shown that there is no molecular support for the current subfamily classifications within the Rutaceae. The Dictyolomatoideae and Spathelioideae belong to a clade separate from the clades containing the remaining Rutaceae subfamilies. Rutoideae and Citroideae do not form discrete clades which suggests a reassessment of the subfamily classification is necessary, particularly asRuta falls within the majority Citroideae clade. Flindersioideae forms a clade within the Rutaceae and does not form a separate family or form a clade with Meliaceae. Sequencing of 17Flindersia species produces a similar phylogeny to that proposed by other authors using morphological methods with two exceptions. The molecular phylogeny indicatesF. amboinensis is associated withF. fournieri andF. laevicarpa and, in addition,F. oppositifolia andF. pimenteliana were found to be genetically identical.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00985324
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