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  • 1
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    European journal of neuroscience 8 (1996), S. 0 
    ISSN: 1460-9568
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: A highly specific monoclonal antibody and pre-embedding immunocytochemistry were employed to examine the distribution of the K+ channel a subunit Kv1.2 in the rat cerebellum. At the light microscopic level, the heaviest immunoreactivity was seen in the basket cell pinceau at the base of Purkinje cells, with lighter staining of basket and Golgi cell bodies and a punctate pattern in the granule cell and molecular layers. Electron microscopy was performed to identify the ultrastructural location of Kv1.2 α subunit in these labelled structures. This revealed that the labelling of the pinceau was confined to the preterminal axonal plexus, the area immediately around the Purkinje axon initial segment being relatively devoid of staining. Basket cell parent axons were not immunostained, but gave rise to heavily stained fine processes. Immunoreactivity was also seen in myelinated axons in the granule cell layer and in the medial cerebellar nucleus, the staining being most concentrated at the juxtaparanodal regions of the axons. An unusual pattern of staining was seen in some mossy fibre terminals, with staining restricted to fine protuberances of mossy fibre glomeruli. Structures contacted by these protuberances included adjoining glial processes. Immunostaining was absent from Purkinje cell bodies, dendrites, their axon initial segments and their terminals in the medial cerebellar nucleus. In this study, the a subunit Kv1.2 was localized to a number of different cell types in the cerebellum. Each neuronal type displays a distinct subcellular distribution of the subunit.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 227 (2000), S. 265-272 
    ISSN: 1573-5036
    Schlagwort(e): Aerenchyma ; Eriophorum vaginatum L. ; methane ; peatland ; Sphagnum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Vegetation composition was found to be an important factor controlling CH4 emission from an ombrotrophic peatland in the UK, with significantly greater (P 〈 0.01) CH4 released from areas containing both Eriophorum vaginatumL. and Sphagnum, than from similar areas without E. vaginatum. Positive correlations were observed between the amount of E. vaginatum and CH4 emission, with the best predictor of flux being the amount of below-ground biomass of this species (r 2 = 0.93). A cutting experiment revealed that there was no significant difference (P 〉 0.05) in CH4 flux between plots with E. vaginatum stems cut above the water table and plots with intact vegetation, yet there was a 56% mean reduction in CH4 efflux where stems were cut below the water table (P 〈 0.05). The effect of E. vaginatum on CH4 release was mimicked by the presence of inert glass tubes. These findings suggest that the main short-term role of E. vaginatum in the ecosystem is simply as a conduit for CH4 release. The longer-term importance of E. vaginatum in controlling CH4 fluxes through C substrate input was suggested by the positive correlation between the night-time CO2 and CH4 fluxes (r 2 = 0.70), which only occurred when the vegetation was not senescent.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 165 (1982), S. 225-248 
    ISSN: 0002-9106
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: Isolated guinea pig adrenocortical cells were maintained in longterm culture in order to perform sequential experiments on the same cell populations. The cells produced fluorogenic steroids, shown by thin-layer chromatography to be at least aldosterone, cortisol, and corticosterone. In addition, they increased production of these steroids when treated with either ACTH or dibutyryl cyclic AMP. Of particular interest was the fact that cultures treated for the initial 24-hour culture period with ACTH maintained enhanced levels of secretion for several days in absence of hormone and had an enhanced response to ACTH later in the culture period. Such enhancement of secretion was not seen following early treatment with dibutyryl cyclic AMP. The fine structure of the ACTH-treated cells was consistent with increased steroidogenesis. They possessed more smooth-surfaced endoplasmic reticulum, larger mitochondrial cristal surfaces, and larger Golgi complexes than the cells in untreated cultures.
    Zusätzliches Material: 16 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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