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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 23 (1988), S. 0 
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Chemotactic N-formyl oligopeptides are produced by bacteria at sites of bacterial colonization in the digestive tract, but are susceptible to degradation by intestinal mucosal and luminal carboxypeptidases. Since production of such peptides by oral bacteria may promote neutrophil leukocyte accumulation in periodontal inflammatory disease, the activity and source of chemotactic peptide-degrading enzymes in human saliva was investigated. An N-formyl-methionyl-leucyl-phenylalanyl carboxypeptidase was identified in whole mixed, but not pure parotid or submandibular saliva. The enzyme activity was attributable to a bacterial rather than a host source.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Inflammation research 18 (1986), S. 550-554 
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A method has been developed for thein vivo measurement of leucocyte chemotaxis in response to the bacterial chemotactic peptide F-met-leu-phe (FMLP). Polyurethane sponges were pre-treated with FMLP and implanted subcutaneously in rats and after a suitable interval removed for determination of leucocyte influx.In vivo concentration gradients of chemotactic factors within intact sponges were shallow and leucocyte accumulation unsatisfactory. Accordingly a cored sponge model was developed in which the cylindrical core only was treated with chemotactic factor and the sponge reassembled prior to subcutaneous implantation. Steep concentration gradients were established within the outer sponge matrix with marked effects on leucocyte accumulation, permitting studies of the time course ofin vivo chemotaxis. With cored sponges test to control cell number ratios were maximal at 4 hours using both free and albumin-bound FMLP. This model ofin vivo chemotaxis may prove useful in several areas of inflammation research.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 45 (1989), S. 1097-1099 
    ISSN: 1420-9071
    Keywords: Formyl peptides ; microsequencing ; E. coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A novel purification procedure for N-formyl methionyl oligopeptides from bacterial culture supernatants has been developed. C-terminal carboxypeptidase microsequencing of purified peptides fromE. coli supernatants enabled identification of 6 naturally occurring formyl-oligopeptides.
    Type of Medium: Electronic Resource
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