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  • 1
    Electronic Resource
    Electronic Resource
    Protein phosphatase type-1 regulatory subunits Reg1p and Reg2p act as signal transducers in the glucose-induced inactivation of maltose permease in Saccharomyces cerevisiae (2000)
    Springer
    Molecular genetics and genomics 263 (2000), S. 411-422 
    Details
    ISSN: 1617-4623
    Keywords: Key words Maltose permease ; REG1 ; 2 ; Glucose signaling ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The REG1 gene encodes a regulatory subunit of the type-1 protein phosphatase (PP1) Glc7 in Saccharomyces cerevisiae, which directs the catalytic subunit to substrates involved in glucose repression. Loss of REG1 relieves glucose repression of many genes, including the MAL structural genes that encode the maltose fermentation enzymes. In this report, we explore the role of Reg1p and its homolog Reg2p in glucose-induced inactivation of maltose permease. Glucose stimulates the proteolysis of maltose permease and very rapid loss of maltose transport activity – more rapid than can be explained by loss of the permease protein alone. In a reg1Δ strain we observe a significantly reduced rate of glucose-induced proteolysis of maltose permease, and the rapid loss of maltose transport activity does not occur. Instead, surprisingly, the slow rate of proteolysis of maltose permease is accompanied by an increase in maltose transport activity. Loss of Reg2p modestly reduces the rates of both glucose-induced proteolysis of maltose permease and inactivation of maltose transport activity. Overexpression of Reg2p in a reg1Δ strain suppresses the effect on maltose permease proteolysis and partially restores the inactivation of maltose transport activity, but does not affect the insensitivity of MAL gene expression to repression by glucose observed in this strain. Thus, protein phosphatase type-1 (Glc7p-Reg1p and Glc7p-Reg2p) plays a role in transduction of the glucose signal during glucose-induced proteolysis of maltose permease, but only Glc7p-Reg1p is involved in glucose-induced inactivation of maltose transport activity and glucose repression of MAL gene expression. Overexpression of REG1 partially restores proteolysis of maltose permease in a grr1Δ strain, which lacks glucose signaling, but does not rescue rapid inactivation of maltose transport activity or sensitivity to glucose repression. A model for the role of Reg1p and Reg2p in glucose signaling pathways is discussed. We also uncovered a previously unrecognized G2/M delay in the grr1Δ but not the reg1Δ strains, and this delay is suppressed by REG1 overexpression. The G1/S delay seen in grr1Δ mutants is slightly suppressed as well, but REG1 overexpression does not suppress other grr1Δ phenotypes such as insensitivity to glucose repression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051185
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  • 2
    Electronic Resource
    Electronic Resource
    New types of cellulose materials obtained by an alternative spinning method (1996)
    Springer
    Fibre chemistry 28 (1996), S. 22-27 
    Details
    ISSN: 1573-8493
    Source: Springer Online Journal Archives 1860-2000
    Topics: Technology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Conclusions ALCERU, Lyocell, Newcell, and Tencel (the order is alphabetical and not by importance) are thus alternative hydrated cellulose fibres fabricated by a method with a low degree of harmfulness. Is this only a name or is it also a hope, as for viscose wool fibre 60 years ago? In our opinion, this is much more than only the beginning of a new generation of fibres, yarns, thin strips from film, spun articles, etc. Is this perhaps an overestimation again? No, we are convinced that this is the true path to future developments. In research on this type of processing, the goal should not be to replace natural silk, cotton, or chemical fibres, but to create a totally different type of biodegradable material fabricated with a low degree of harmfulness of the process. A multitude of requirements of modern economic development related to the operation and cost-effectiveness of production could be satisfied in this way. During development, we became convinced that fibrillation is not a defect, but instead a positive effect whose use will make it possible to discover new types of materials. And we should now be bold in using these new fibres and yarns for creating new materials. The reports from Courtaulds (Great Britain) concerning the introduction of three installations with a capacity of 50,000 tons a year each and Lenzing (Austria) concerning an installation with a capacity of 40,000 tons a year in the next three years are convincing evidence of the validity of this opinion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01130693
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    Paper (German National Licenses)
    Fulltext
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