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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 32 (1993), S. 101-107 
    ISSN: 1573-5044
    Keywords: hypocotyl ; IAA-oxidase ; isozyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hypocotyl explants of Linum usitatissimum were induced to form roots without an intermediate eallus phase by incubation on a defined medium. Loosely bound and ionically bound surface-associated proteins were extracted from the explants during root development by sequential vacuum infiltration using distilled water and 100 mM calcium chloride solution. The ionically extracted samples generally had higher peroxidase activity than the secreted samples, but both had reached maxima after 28 days culture. In contrast, the secreted samples were more able to oxidise indole-3-acetic acid (IAA) than the ionically-extracted samples. After 14 days culture the peroxidase and IAA-oxidase activities of the two samples were approximately equal, but by 35 days the secreted sample was twice as effective in oxidising IAA as the ionically extracted sample. The results suggest an accumulation of a loosely associated IAA-oxidase/peroxidase on the surfaces of the explants during root growth and development. Five anionic (A1–A5) and five cationic (C1−C5) isozymes were identified using non-denaturing PAGE. All five anionic isozymes were present throughout the development of roots and became more abundant from 14 days to 35 days culture. In contrast, root development was accompanied by a reduction in the levels of cationic isozymes that are characteristic of hypocotyl tissue. Two cationic isozymes (C3 and C4) were exclusively present during the early phases of root development (14 days) and the other three cationic isozymes were present at 14 days, dropped in abundance at 21 days and then recovered to higher levels after 35 days. The possible roles and consequence of these cationic isozymes and the significance of their removal from the explant surface during root development is discussed.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5044
    Keywords: differentiation ; flax ; maltose ; organogenesis ; regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three carbohydrate sources, maltose, sucrose and cellobiose, were compared for the respective effects on regeneration from internodal sections of flax. It was found that different patterns of morphogenesis were attributable to the type of carbohydrate and its concentration. Mean shoot regeneration was highest on a medium containing 87 mM maltose with a rate of 2.4 shoots per explant and root formation was stimulated to the greatest degree on a medium with 87 mM sucrose. This medium also resulted in the highest tissue dry weight.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-5044
    Keywords: Brassica napus ; protoplasts ; Ficoll ; agarose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method is described for regenerating callus from mesophyll protoplasts of a winter variety of Brassica napus. The method combines the use of Ficoll in an initial liquid medium, enhancing early protoplast division and cell colony formation, with a transfer to an agarose system after 10 days culture to give rapid microcalli formation. Further transfers resulted in callus regeneration and the initiation of organogenesis.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 24 (1991), S. 43-47 
    ISSN: 1573-5044
    Keywords: Brassica napus ; protoplasts ; purification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of three different general purification protocols have been assessed quantitatively using mesophyll protoplasts of Brassica napus. Within the initial sample two distinct sub-populations were determined. The methods used influenced the ratio of the vacuolated to chloroplastic type protoplast sub-populations. Overall recovery rates of the initial sample varied according to the method used from 38% to 27%, but the relative recovery of the sub-populations varied considerably with a purified ratio of between 1.0:0.78 to 1.0:7.0. Size distribution profiles of the initial and purified populations are also presented.
    Type of Medium: Electronic Resource
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