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  • 1
    Electronic Resource
    Electronic Resource
    Rotational spectrum of a dark state in 2-fluoroethanol using microwave/radio-frequency-infrared multiple resonance (1994)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 100 (1994), S. 831-839 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Microwave/radio-frequency-infrared multiple resonance has been used with an electric-resonance optothermal spectrometer to characterize a weak 21.6 MHz perturbation in the infrared spectrum of the ν14 C–O stretching vibration of 2-fluoroethanol. The infrared spectrum of 2-fluoroethanol was recorded at a resolution of ∼2 MHz using a tunable microwave-sideband CO2 laser. The spectrum is fit by an asymmetric-rotor Hamiltonian to a precision of 0.6 MHz, except for the transitions to the 413 upper state which are split into doublets by an interaction between the 413 level and a rotational level of a nearby background, or dark, vibrational state. Microwave/radio-frequency-infrared double and triple resonance reveals that the 413 level of the C–O stretching vibration is interacting with the 431 level of the dark state. The rotational constants determined for the dark state allow us to assign the perturbing state to the ν18+4ν21 combination vibration of the lowest energy conformer, where ν18 is the CCO bending vibration and ν21 is the C–C torsional vibration. From the weak ΔKa=2 matrix element between ν14 and ν18+4ν21 it is possible to derive a J=0 anharmonic interaction between these states of ∼3.5 GHz.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.466566
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  • 2
    Electronic Resource
    Electronic Resource
    Essential Fatty Acids and Polyunsaturated Fatty Acids: Significance in Cutaneous Biology (1990)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Nutrition 10 (1990), S. 433-450 
    Details
    ISSN: 0199-9885
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.nu.10.070190.002245
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  • 3
    Electronic Resource
    Electronic Resource
    Validation of a morphometric method for evaluating fibroblast numbers in normal and pathologic tissues (2003)
    Oxford, UK : Munksgaard International Publishers
    Experimental dermatology 12 (2003), S. 0 
    Details
    ISSN: 1600-0625
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract:  The aim of this work was to validate an image analysis method, based on cell nuclei form factor determination, for counting fibroblasts within human dermis. We first used reconstructed dermal equivalents in which fibroblasts can also be counted directly after lysis of the collagen matrix. We found a good correlation between the results of direct counting and those of image analysis from day 10 to day 28 of culture. When applied to young normal donors' skin biopsies fixed in Bouin's solution and embedded in paraffin, the image analysis method yielded mid-dermis fibroblast counts of between 2100 and 4100 per mm3 of fresh tissue. A nuclear form factor (FF) comprised between 0.35 and 0.84 was found to be a biologic marker of fibroblasts. This was confirmed after fibroblast discrimination from other cell types, which had rounder nuclei (FF ≥ 0.85) and were identified either by their location (e.g. endothelial cells) or by labeling with specific antibodies (e.g. lymphocytes and monocytes/macrophages). Similar results were obtained with seven healthy donors' skin biopsies that had been frozen in nitrogen liquid and cryostat-sectioned, showing that this counting method is independent of the histologic procedure. Finally, analysis of samples of hypertrophic scars from two patients revealed that fibroblast density in some parts of the dermis was more than twice the value found in other parts presenting a fibroblast density almost normal, showing that this cell counting method can also be used to assess fibroblast heterogeneity within a given tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0625.2003.00023.x
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  • 4
    Electronic Resource
    Electronic Resource
    Staining with monoclonal antibodies to neurofilaments distinguishes between subpopulations of neurofibrillary tangles, between groups of axons and between groups of dendrites (1987)
    Springer
    Journal of neurology 234 (1987), S. 241-246 
    Details
    ISSN: 1432-1459
    Keywords: Alzheimer's disease ; Neurofibrillary tangles ; Neurofilament ; Monoclonal antibody ; Olfactory bulb
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A new monoclonal antibody (mab) against neurofilaments is described (mab 1215) and its reactions compared with previously characterized mabs (BF10; RT97). Mab 1215 recognizes an epitope on the heavy neurofilament polypeptide (NF-H). In Alzheimer's disease, mab 1215 recognizes only a subpopulation of neurofibrillary tangles and stains a proportion of tangles in the hippocampus but none of those in the olfactory bulb. However, mabs RT97 and BF10 stain the majority of tangles in both brain areas. Of the three antibodies, only mab BF10 recognizes, specifically, axons of granular cells in the dentate gyrus of the hippocampus. Mab 1215 recognizes more dendrites in the pyramidal layer than either mab BF10 or mab RT97. Our observations indicate that neurofilaments are not identical in all axons and that, contrary to previous reports, NF-H is present in dendrites. The dendritic form of NF-H appears to be different from NF-H in axons and this could be due to differences in the state of phosphorylation of NF-H. We suggest that the finding that distinct subpopulations of tangles exist indicates that tangles are not static lesions. Further investigations into this possibility may illuminate the pathophysiology of Alzheimer's disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00618257
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  • 5
    Electronic Resource
    Electronic Resource
    Selection, linkage, and dominance in small populations (1971)
    Springer
    Theoretical and applied genetics 41 (1971), S. 130-135 
    Details
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Linkage, dominance, and selection interact significantly to alter the mean coefficient of inbreeding. The effect of one is not predictable without the other two. Close linkage between adjacent loci in the presence of intense selection caused a different response with overdominant gene action from with partial dominance. When selection was random, effects of linkage and dominance on the coefficient of inbreeding were nonexistent; but when selection was by either phenotype or genotype, linkage and dominance became important. Joint effects between linkage, dominance, and selection are illustrated in specific simulated populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00277754
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  • 6
    Electronic Resource
    Electronic Resource
    Monoclonal antibodies against plant proteins recognise animal intermediate filaments (1987)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 8 (1987), S. 312-323 
    Details
    ISSN: 0886-1544
    Keywords: plant cytoskeleton ; Chlamydomonas ; anti-IFA ; onion root tip cells ; immunoflurescence ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Four monoclonal antibodies were raised against polypetides present in a highsalt detergent-insoluble fraction from cells of Chlamydomonas reinhardtii. Indirect immunofluorescence microscopy of fibroblasts and epithelial cells grown in culture using these plant antibodies revealed staining arrays identical to those obtained with well characterised antibodies to animal intermediate filaments. Immunoflurescence microscopy of Chlamydomonas with these monoclonal antibodies and a monoclonal antibody that recognises all animal intermediate filaments (anti-IFA) gave a diffuse, patchy cytoplasmic staining pattern. Both the plant antibodies and anti-IFA stained interphase onion root tip cells in a diffuse perinuclear pattern. In metaphase through to telophase, the labelling patterns colocalised with those of microtubules. Labelling of the phragmoplast was also detected but not staining of the preprophase band. On Western blots of various animal cell lines and tissues, all the antibodies labelled known intermediate filament proteins. On Western blots of whole Chlamydomonas proteins, all the antiboides labelled a broad band in the 57,000 Mr range, and three antibodies labelled bands around 66,000 and 140,000 Mr but with varibale intensites. On Western blots of whole onion root tip proteins, all the antibodies labelled 50,0000 Mr (two to three bands) polypetides and a diffuse and around 60,000 Mr and three of the antibodies also labelled several polypeptides in the 90,000-200,000 Mr range. The consistent labelling of these different bands by several different monoclonal antibodies recognising animal intermediate filaments makes these polypetides putative plant intermediate filament proteins.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970080404
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  • 7
    Electronic Resource
    Electronic Resource
    Viscosimetrie (1938)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 113 (1938), S. 443-446 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01602299
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  • 8
    Electronic Resource
    Electronic Resource
    Cytoplasmic deletion processes during spermatogenesis in mosses (1986)
    New York, NY : Wiley-Blackwell
    Gamete Research 13 (1986), S. 253-270 
    Details
    ISSN: 0148-7280
    Keywords: mosses ; Sphagnum ; cytoplasmic deletion ; Golgi ; endoplasmic reticulum ; vacuoles ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Comparative ultrastructural observations reveal that cytoplasmic deletion during spermatogenesis in Sphagnum and other mosses (Bryopsida) has two distinct phases. In young spermatids, Golgi-derived vesicles produce the mucopolysaccharide sheaths in which the gametes are liberated. Golgi bodies, however, play no part in removal of cytoplasm during gamete maturation. Rounding off of the cells during this process results in a 50% reduction in volume.Mid-spermatid stages in Sphagnum are characterised by the sequential loss of Golgi bodies and endoplasmic reticulum (ER) but no further diminution of the cytoplasm. The final stages of nuclear metamorphosis and chromatin condensation, in late spermatids, are marked by the sudden appearance, in the otherwise featureless central cytoplasm, of a membrane vesicle complex (MVC) comprising cisternae, tubules, and smooth and coated vesicles. Following repositioning of the MVC beneath the plasma membrane, rapid shrinkage of the cytoplasm is associated with the presence of vesicle fusion profiles at the cell surface. The MVC is considered to be intimately involved in cytoplasmic breakdown and loss. Acid phosphatase activity can be detected throughout spermatogenesis. Spermatogenous cells and young spermatids possess relatively low levels of the enzyme, restricted to the ER and perinuclear space, but particularly high levels occur in the MVC region of late spermatids of Sphagnum.The deletion process in Bryopsida is much more gradual than that of Sphagnum. Mid-spermatids contain sheets of ER, Golgi with small vesicles, and irregular cisternae associated with coated vesicles. Vacuoles derived either from dilation of the ER or the coated vesicle complexes gradually increase in size and number at the expense of the cytoplasm. During the early stages of chromatin condensation, a large central vacuole opens onto the anterior face of the gametes. Further discharge of vesicles continues throughout gamete maturation.A comparative survey of spermatogenesis in land plants indicates that cytoplasmic deletion is achieved in different ways in different groups. We speculate that the spermatozoids of the common ancestor of archegoniate plants probably possessed large amounts of cytoplasm. The deletion mechanisms may have originated from a contractile vacuole apparatus.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120130308
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