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  • 1
    ISSN: 1432-1211
    Keywords: Key words Membrane cofactor protein ; Testis ; Chromosome 1 ; Complement ; Alternative splicing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Human membrane cofactor protein (MCP, CD46) is widely distributed and is one of the plasma membrane complement inhibitors. We isolated cDNA clones encoding genetic homologues of human MCP from a rat testis cDNA library. Northern blot analysis indicated that rat MCP is preferentially expressed in testis, similar to what is found with guinea pig MCP. We identified several different cDNAs, which were presumably generated by alternative splicing from a single-copy gene. The most prevalent isoform corresponded to the Ser/Thr/Pro-rich C type of human MCP. Mouse MCP cDNA was cloned by polymerase chain reaction based on the nucleotide sequence of rat MCP. The deduced amino acid sequence showed 77.8% identity to rat MCP. Mouse MCP was also preferentially expressed in testis. Unique expression in testis in rat and mouse as well as guinea pig suggests that MCPs in these species not only act as complement regulatory proteins but may also have more specialized functions in fertilization or reproduction. Genetic mapping by linkage analysis indicated that the mouse Mcp gene is located on distal chromosome 1, closely linked to the complement receptor 2 (Cr2) gene.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Subsurface sensing technologies and applications 1 (2000), S. 161-175 
    ISSN: 1573-9317
    Keywords: borehole radar ; radar polarimetry ; stepped-frequency radar system ; polarimetric calibration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: Abstract Radar polarimetry is a technology that overcomes the limitation between the radar resolution and the penetration depth of borehole radar. We have developed a stepped-frequency polarimetric borehole radar system. This is a polarimetric borehole radar system which measures the full-radar polarimetry in a borehole by changing the antenna arrangements. By using a network analyzer and an optical analog signal link, this system has a frequency bandwidth of 2–500MHz, which is suitable for two different antennas for radar polarimetry. We also propose a technique for polarimetric antenna calibration. In order to understand the potential of polarimetric borehole radar, field measurement were carried out at the Mirror Lake fractured-rock research site (NH, USA). The radar penetration depth from the borehole in the reflection measurement was over 10m, at the frequency range of 2–402MHz. We observed many clear reflections from fractures in each polarization status. Even in the raw data, we found the differences in the radar profile for different polarization status. We believe the polarimetric feature is closely related to the roughness of each fracture, and it is also related to the physical properties of the fracture such as water permeability.
    Type of Medium: Electronic Resource
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