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  • 1
    Electronic Resource
    Electronic Resource
    Continuous measurement of the cytoplasmic pH in Lactococcus lactis with a fluorescent pH indicator
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/General Subjects 1115 (1991), S. 75-83 
    Details
    ISSN: 0304-4165
    Keywords: (L. lactis) ; Carboxyfluorescein ; Fluorescent probe ; Intracellular pH ; Multidrug resistance ; pH regulation
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0304-4165(91)90014-8
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  • 2
    Electronic Resource
    Electronic Resource
    Side-firing devices for laser prostatectomy (1995)
    Springer
    World journal of urology 13 (1995), S. 88-93 
    Details
    ISSN: 1433-8726
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Transurethral laser coagulation of the prostate has become an accepted treatment for benign prostatic hyperplasia (BPH). The most common method is the use of a sideward-firing fiber that, once inserted in the prostatic area, irradiates the abundant prostatic tissue with Nd: YAG laser light. In this study, eight different side-firing fibers that are commercially available were evaluated. The devices can be characterized by the way laser light is deflected sideward and by their thermal behavior. Most of the eight devices differ with regard to the angle at which the laser beam is deflected, the spot size on the irradiated tissue surface, and the heating of the device itself. Implementation of the optical and thermal characteristics of each device in the treatment protocol will contribute to the optimal use of laser energy for prostatectomy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00183620
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA (1999)
    Springer
    Applied microbiology and biotechnology 52 (1999), S. 541-545 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract An improved method for the electrotransformation of wild-type Corynebacterium glutamicum (ATCC 13032) is described. The two crucial alterations to previously developed methods are: cultivation of cells used for electrotransformation at 18 °C instead of 30 °C, and application of a heat shock immediately following electrotransformation. Cells cultivated at sub optimal temperature have a 100-fold improved transformation efficiency (108 cfu μg−1) for syngeneic DNA (DNA isolated from the same species). A heat shock applied to these cells following electroporation improved the transformation efficiency for xenogeneic DNA (DNA isolated from a different species). In combination, low cultivation temperature and heat shock act synergistically and increased the transformation efficiency by four orders of magnitude to 2.5 × 106 cfu μg−1 xenogeneic DNA. The method was used to generate gene disruptions in C. glutamicum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051557
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    Paper (German National Licenses)
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