Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Comparison of bacterial diversity along the human intestinal tract by direct cloning and sequencing of 16S rRNA genes (2005)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 54 (2005), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Bacterial diversity of the mucosal biopsies from human jejunum, distal ileum, ascending colon and rectum were compared by analysis of PCR-amplified 16S rDNA clone libraries. A total of 347 clones from the mucosal biopsies were partially sequenced and assigned to six phylogenetic phyla of the domain Bacteria: Firmicutes, Bacteroidetes, Proteobacteria, Fusobacteria, Verrucomicrobia, and Actinobacteria. The jejunum sample had least microbial diversity compared to the other samples and a trend towards highest diversity in ascending colon was observed. The clone libraries of distal ileum, ascending colon and rectum were not significantly different from each other (P 〉 0.0043), but they differed significantly from the jejunum library (P= 0.001). The population of sequences retrieved from jejunal biopsies was dominated by sequences closely related to Streptococcus (67%), while the population of sequences derived from distal ileum, ascending colon and rectum were dominated by sequences affiliated with Bacteroidetes (27–49%), and Clostridium clusters XIVa (20–34%) and IV (7–13%). The results indicate that the microbial community in jejunum is different from those in distal ileum, ascending colon and rectum, and that the major phylogenetic groups are similar from distal ileum to rectum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsec.2005.03.012
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Effect of temperature and glucose supply on the production of polyunsaturated fatty acids by the fungus Mortierella alpina CBS 343.66 in fermentor cultures (1993)
    Springer
    Applied microbiology and biotechnology 39 (1993), S. 450-455 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The fungus Mortierella alpina CBS 343.66 was grown in a fermentor at different pH, temperatures and supplies of carbon source (glucose) in order to optimize the culture conditions for rapid biomass and lipid production with a high proportion of polyunsaturated fatty acids, especially arachidonic acid (AA). Good growth and lipid production with 31% AA was obtained at pH 6.5 and 25°C. A temperature decrease to 18°C gave a significantly higher degree of polyunsaturated fatty acids. Eicosapentaenoic acid was not detected at 25°C, but was formed at 18°C at about 10%. The AA concentration but was similar for 18°C about 10%. The AA concentration was similar for 18°C and 25°C (around 30%): 18°C allowed relatively good growth and had a beneficial effect on the fungus morphology, i.e. pellets were formed. Best lipid production and a AA content of up to 33% was achieved at an excess of glucose (carbon source) and a deficit of ammonium chloride (nitrogen source). The percentage of AA of the total fatty acid composition was constant as ong as glucose was present. At glucose exhaustion, the proportion of AA increased to 57%. The increase in AA corresponded to a decrease in palmitic acid, stearic acid and oleic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00205031
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Capacity for biosurfactant production of environmental Pseudomonas and Vibrionaceae growing on carbohydrates (1987)
    Springer
    Applied microbiology and biotechnology 26 (1987), S. 439-442 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Pseudomonas and Vibrionaceae strains with the capacity to produce biosurfactants when growing on sucrose were isolated from the environment by a simple screening procedure. Agargrown colonies were randomly selected; each colony was suspended in a water droplet on a microscope slide. The tested strain was regarded as positive if the droplet spread over the surface. 1779 Pseudomonas and 660 Vibrionaceae isolates were tested; 1% and 0.8% of the isolates, respectively, were positive for biosurfactant production. No production was detected amongst the isolates of a control group of 538 Gram-positive and 1063 Gram-negative strains. Four biosurfactant producing strains were grown in fermenter cultures on a semisynthetic medium using sucrose as carbon and energy source. The terminal concentrations of biosurfactants were in the range of a factor 40 times the critical micelle dilution. One P. fluorescens strain was grown in a carbon limited chemostat (succinate). The biosurfactant production was successively decreasing until it stopped after less than ten generation times.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00253528
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    The aerobic growth and product formation of Lactobacillus, Leuconostoc, Brochothrix, and Carnobacterium in batch cultures (1989)
    Springer
    Applied microbiology and biotechnology 30 (1989), S. 81-88 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The aerobic growth and metabolism of eleven homofermentative and three heterofermentative Lactobacillus strains, three Leuconostoc strains, two Brochothrix thermosphacta strains and two Carnobacterium strains were studied in batch cultures at pH 6.0 and 25°C on a complex substrate containing 10.0 g glucose per litre. All strains, except Carnobacterium divergens 69, grew well aerobically. An oxygen consumption was registered for 18 of the strains—the exceptions being Lactobacillus alimentarius DSM 20249T, Lactobacillus farciminis DSM 20284T and Lactobacillus sharpeae DSM 20505T. The homofermentative lactobacilli showed a maximal oxygen consumption during the stationary growth phase and this was coupled with a low final viable count. Leuconostoc strains, heterofermentative lactobacilli, Brochothrix thermosphacta and Carnobacterium strains showed a maximal oxygen consumption during the exponential growth phase together with a high final viable count. The maximum specific growth rate varied from 0.19 to 0.54 h-1 while the growth yield varied from 19 to 86 g dry weight per mol glucose consumed. In general, homofermentative lactobacilli produced dl-lactic acid, acetic acid and acetoin. The three heterofermentative lactobacilli produced dl-lactic acid and acetic acid, two strains also produced ethanol Leuconostoc spp. formed d-lactic acid, acetic acid, and ethanol. B. thermosphacta produced acetoin, acetic acid, formic acid, isobutyric acid and isovaleric acid but no lactic acid. Carnobacterium produced l-lactic acid, acetic acid and acetoin. All strains accumulated hydrogen peroxide except L. alimentarius DSM 20249T, Carnobacterium piscicola 3 and B. thermosphacta.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00256001
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Conversion of fat into yeast biomass in protein-containing waste-water (1990)
    Springer
    Applied microbiology and biotechnology 33 (1990), S. 473-476 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Candida tropicalis S001 was grown on the lipid fraction of a protein-containing waste-water in order to (i) remove fat from the water, and (ii) produre yeast biomass for feed. The yeast cells were separated from the waste-water by sedimentation. Defatted waste-water was used for methane production and gave a yield of a 0.3 m3 methane/kg reduced chemical oxygen demand. The maximum specific growth rate (µmax) of C. tropicalis growing on waste-water fat at pH 4.0 was 0.35 h−1; the fat content was decreased from 8 g/l to about 0.1 g/l within 24 h. In continous culture a corresponding reduction was maintained at dilution rates up to 0.36 h−1. The effect on growth of pH, temperature and CO2 concentration was studied with triolein as the major carbon source. The µmax was nearly constant (0.16 h−1) in the pH and temperature range of 3.2–4.0 and 30°–38° C, respectively; 10% CO2 was optimal for growth. Growth on triolein resulted in a biomass yield of 0.70 g dry weight/g fat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00176669
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Screening for lipase-producing microorganisms with a continuous cultivation system (1991)
    Springer
    Applied microbiology and biotechnology 35 (1991), S. 19-20 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The enrichment of lipase-producing bacteria, isolated from the environment, was evaluated using a continuous cultivation system. Continuous cultivations were performed using a synthetic medium where the carbon and energy source, triolein, was provided physically entrapped in an external loop. Using this system, pure cultivations of Pseudomonas fluorescens 378 showed an increase in lipase-producing ability, instead of declining as in more conventional systems. Enrichment cultures were obtained with environmental samples originating from a vegetable oil processing plant using the same system. Three types of lipase-producing bacteria were identified: P. alcaligenes, Enterobacter intermedium and a Gram-negative, oxidase-positive rod.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00180629
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Biofilm build-up of Pseudomonas putida in a chemostat at different dilution rates (1982)
    Springer
    Applied microbiology and biotechnology 15 (1982), S. 218-222 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A test system was set up where the build-up of a biofilm on a defined surface could be studied in a carbon source limited chemostat. The attachment of P. putida ATCC 11172 to glass when growing on L-asparagine was studied at different dilution rates (specific growth rates) from 0.1 to 1.5 h−1 The number of attached colony forming units (cfu) increased with dilution rate from 1×106 cfu/cm2 at 0.1 h−1 to 4×107 cfu/cm2 at 1.0 h−1 and then the attachment decreased to about 6×106 cfu/cm2 at higher dilution rates (1.1–1.5 h−1). The number of attached cfu was measured after 24 h exposure. The value of the maximum specific growth rate in batch culture was 0.6 h−1. The total amount of attached cell-mass followed roughly the same pattern as the viable count. The viable count of the cells suspended in the growth medium showed its lowest value at the same dilution rate as resulted in maximum adhesion. It was shown that the effect of growth rate on the biofilm build-up of P. putida is significant, and ought to be borne in mind when continuous culture systems are set up and results evaluated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00499959
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    The impact of dilution rate and attached growth on steady-state characteristics of Pseudomonas putida (1981)
    Springer
    Applied microbiology and biotechnology 13 (1981), S. 102-106 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Pseudomonas putida (ATCC 111 72) was studied in a continuous culture at various dilution rates with asparagine as the sole carbon source and limiting factor. Under the experimental conditions applied, a considerable number of the cells became attached to the fermentor walls and equipment. The viable count of the attached cells was of the same magnitude as those in suspension. The following steady-state characteristics were obtained: The cell-mass (OD620 and dry weight) versus dilution rate (D) had maxima at 0.63 and 1.1 h−1. The corresponding plot of viable count had a minimum at 0.94 h−1 whereafter it reached a maximum at 1.3 h−1. Largest yield coefficient obtained was 0.44 g dry weight/g asparagine (D=1.1 h−1). The productivity of the culture increased with D up to 1.1 h−1, which is far above the D corresponding to the maximum specific growth rate (μmax) of a batch culture (0.59 h−1). The cell mass was not completly washed-out of the fermentor even at a D of 2.2 h−1. The influence of attached growth for the steady-state characteristics, and the significance of the results in relation to chemostate as an instrument for testing environmental factors, are discussed. It is suggested that the attached cells had a significantly higher (μmax) value than the suspended ones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00499696
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Effect of different environmental parameters on the biofilm build-up of Pseudomonas putida ATCC 11172 in chemostat (1983)
    Springer
    Applied microbiology and biotechnology 18 (1983), S. 114-119 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The influence of carbon source, cell concentration, oxygen tension, pH and temperature on the biofilm build-up of Pseudomonas putida ATCC 11172 was studied. The experiments were performed in a carbon and energy limited chemostat (asparagine). When the asparagine was replaced by glucose the biofilm build-up was decreasing. Cell concentration, oxygen tension or temperature did not to any significant degree affect the biofilm build-up. Temperature, however influenced the size of the suspended cells. The cell size successively increased with decreasing temperature. A similar change in cell size could be accomplished by increasing the dilution rate from 0.2 h−1 (small cells) to 0.7 h−1 (large cells). The biofilm build-up increased with increasing pH in the interval of 5.5–6.7. The viable count of the biofilm after 24 h exposure of test surface increased from 8×106 cfu/cm2 to 1.6×108 cfu/cm2 while the biomass increased by a factor of about 70.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00500834
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Measurement of the maximum specific growth rate in chemostat of Pseudomonas spp. with different abilities for biofilm formation (1983)
    Springer
    Applied microbiology and biotechnology 18 (1983), S. 303-307 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Attempts to measure the maximum specific growth rate (μmax) using the method of wash-out were made on two Pseudomonas spp. with different abilities for biofilm formation. The apparent “μmax” of the strain with the highest biofilm activity (P. putida ATCC 11172) was governed by the dilution rate of the steady-state preceding the wash-out experiment. The apparent “μmax” was increased from 0.4 to 3.8 h−1 by changing the dilution rate of the preceding steady-state from 0.3 to 2.5 h−1. The μmax determined from a single batch culture was 0.59 h−1. The apparent “μmax” of the strain with the lower biofilm ability (P. fragi 72) was also influenced, but to a lesser extent, by the steady-state preceding the wash-out experiment. The apparent “μmax” was altered from 0.39 h−1 to 0.59 h−1 by changing the preceding dilution rate from 0.34 h−1 to 0.49 h−1. It was experimentally demonstrated that μmax cannot be measured in a carbon and energy limited chemostat using the wash-out method, if the organism has any tendency towards biofilm formation. It was also indicated that the reciprocal plot of specific growth rate against substrate concentration, using data from a chemostat with attached growth, was unlinear and hence μmax could not be calculated in this way. It is suggested that instead of using invalidly estimated μmax as a measurement of the “maximal growth capacity” of an organism in continuous culture, the dilution rate where the biomass of the steady-state starts to decrease should be given.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00500496
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...