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  • 1
    Electronic Resource
    Electronic Resource
    Molecular characterization of the Cassava brown streak virus coat protein (2001)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 50 (2001), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A partial sequence of 1114 nucleotides of a virus from cassava brown streak diseased (CBSD) material was obtained. Alignment of the predicted amino acid sequence with those of other members of the Potyviridae showed closest identity with the coat protein of Sweet potato mild mottle virus (genus Ipomovirus). The predicted amino acid sequence has one open reading frame with a 3′ untranslated region of 144 nucleotides and a poly(A) tail. The expressed protein was shown to cross-react with an antiserum raised previously to a virus isolated from CBSD material. Evidence presented suggests that CBSD is caused by Cassava brown streak virus, a tentative member of the genus Ipomovirus, as this virus is consistently found associated with CBSD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.2001.00589.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of different isolates of Cassava brown streak virus and development of a diagnostic test (2001)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 50 (2001), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: An RT-PCR based detection method for Cassava brown streak virus (CBSV)-infected cassava has been developed. The RT-PCR detection method described includes RNA extraction methods for cassava leaves, a distinct primer set for the virus and RT-PCR conditions. The primers were designed to the virus coat protein gene and generate a virus-specific product of 231 bp from infected cassava. The test can detect the virus in the new growth of cassava sticks before any disease symptoms are visible. This test was used successfully with infected cassava from both Tanzania and Mozambique. Three isolates from Tanzania were found to exhibit different symptoms on the secondary host plants Nicotiana benthamiana and N. tabacum SR1. They have nucleotide sequence variation within the coat protein region of up to 8% and amino acid differences of up to 6%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.2001.00647.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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