ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

A highly conserved nucleotide string shared by all genomes of human papillomaviruses (1991)

Campione-Piccardo, José ; Montpetit, Michael L. ; Grégoire, Lucie ; [et al.]

Springer

Virus genes 5 (1991), S. 349-357

add to mindlist on the mindlist

Details

ISSN: 1572-994X

Keywords: papillomavirus ; consensus sequence ; sequence evolution ; oligonucleotide

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract The nucleotide string TAAAACGAAAGT is the longest perfect homology shared by all sequenced human papillomavirus genomes. This nucleotide string, which was also found to be highly specific for human papillomavirus genomes, shares the same genomic position in all viral types (5′ end of the E1 open reading frame) and putatively codes in every case for the same amino acids. One possible evolutionary model was used to estimate the probability of random occurrence of the nucleotide string in 10 human papillomavirus genomes. It assumed that the universal string had been subjected to the same mutation rate as the entire E1 open reading frame. The estimated probability was found to be very low, suggesting that the conservation of the string could not have resulted from random divergence and that its conservation among human papillomaviruses is likely to reflect the occurrence of biological constraints. It is speculated that this nucleotide string may be required to code for amino acids indispensable for the nuclear localization of E1-coded peptides or to bind cellular factors affecting viral replicative functions. Definitive evidence is expected to come from oligonucleotide-protein binding experiments and from site-directed mutagenesis of cloned HPV genomes. This motif, universal among human papillomaviruses, is being successfully used in the design of consensus primers from the early region.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00271533

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Does the lack of regression-associated mRNA expression render a rat ventral prostate epithelial cell line androgen independent? (1989)

Montpetit, Michael L. ; Tenniswood, Martin P.

New York, N.Y. : Wiley-Blackwell

Journal of Cellular Biochemistry 39 (1989), S. 285-292

add to mindlist on the mindlist

Details

ISSN: 0730-2312

Keywords: epithelial cells ; putative growth factor ; regression sequence ; androgen-independent epithelial cells ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology , Medicine

Notes: A series of rapidly dividing epithelial (RDE) cell lines have been isolated from primary cultures of rat ventral prostate (RVP) epithelial cells. Unlike androgen-dependent secretory epithelial cells, the RDE cells in culture do not express the androgen-dependent secretory proteins, nor do they express the androgen-repressed cell death sequences (TRPM-2) found in the epithelial cells during prostatic regression. Screening of a cDNA clone library established from RDE cell mRNA has yielded a number of RDE cell-specific sequences. One of these, RDE-.25 is a 250-base mRNA. The sequence of RDE-.25 shows considerable homology with the rat growth hormone gene and two murine oncogene sequences. We believe that the absence of androgen-repressed cell death sequence expression confers androgen independence for survival and growth, while the expression of RDE-.25 may represent an autocrine growth stimulus which greatly increases the rate of cell division in these cells.

Additional Material: 4 Ill.