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  • 1
    Electronic Resource
    Electronic Resource
    GETTING STARTED:Regulating the Initiation of DNA Replication in Yeast (1997)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Microbiology 51 (1997), S. 125-149 
    Details
    ISSN: 0066-4227
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Notes: Abstract Initiation of DNA replication in yeast appears to operate through a two-step process. The first step occurs at the end of mitosis in the previous cell cycle, where, following the decrease in B cyclin-dependent kinase activity, an extended protein complex called the prereplicative complex (pre-RC) forms over the origin of replication. This complex is dependent on the association of the Cdc6 protein with the Origin Recognition Complex (ORC) and appears concomitantly with the nuclear entry of members of the Mcm family of proteins. The second step is dependent upon the cell passing through a G1 decision point called Start. If the environmental conditions are favorable, and the cells reach a critical size, then there is a rise in G1 cyclin-dependent kinase activity, which leads to the activation of downstream protein kinases; the protein kinases are, in turn, required for triggering initiation from the preformed initiation complexes. These protein kinases, Dbf4-Cdc7 and Clb5/6(B-cyclin)-Cdc28, are thought to phosphorylate targets within the pre-RC. The subsequent rise in B cyclin protein kinase activity following Start not only triggers origin firing, but also inhibits the formation of new pre-RCs, which ensures that there is only one S phase in each cell cycle. The destruction of B-cyclin protein kinase activity at the end of the cell cycle potentiates the formation of new pre-RCs-resetting origins for the next S phase. "... Or say that the end precedes the beginning." TS Eliot
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.micro.51.1.125
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  • 2
    Electronic Resource
    Electronic Resource
    Polo kinase controls cell-cycle-dependent transcription by targeting a coactivator protein (2006)
    [s.l.] : Nature Publishing Group
    Nature 444 (2006), S. 494-498 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Polo kinases have crucial conserved functions in controlling the eukaryotic cell cycle through orchestrating several events during mitosis. An essential element of cell cycle control is exerted by altering the expression of key regulators. Here we show an important function for the polo ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature05339
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  • 3
    Electronic Resource
    Electronic Resource
    Direct evidence for rifampicin-promoted readthrough of the partial terminator tL7 in the rpoBC operon of Escherichia coli (1987)
    Springer
    Molecular genetics and genomics 210 (1987), S. 358-363 
    Details
    ISSN: 1617-4623
    Keywords: Transcription ; Termination ; Rifampicin ; Readthrough ; rpoBC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The RNA polymerase subunits β and β′ of Escherichia coli, encoded by the genes rpoB and rpoC, are co-transcribed with four 50 S ribosomal protein genes, rplKAJL. After treatment with the antibiotic rifampicin a partial uncoupling of rpoBC from rplKAJL transcription occurs. We have been investigating the role played in uncoupling by tL7, an 80% efficient terminator of transcription present in the 319 bp intercistronic space between rplL and rpoB, using S1 nuclease mapping of transcripts produced in vivo in normal (rpoBC haploid) strains. Our results show directly that rifampicin stimulates readthrough of tL7 on the chromosome by approximately twofold, an effect sufficient to explain the observed increase in ββ′ protein synthesis. We also provide preliminary evidence for the map position of PL7, and show that both this and Pβ, two very weak promoters which might in principle be activated by rifampicin, are not in fact stimulated by the drug.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00325706
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  • 4
    Electronic Resource
    Electronic Resource
    DNA synthesis control in yeast: An evolutionarily conserved mechanism for regulating DNA synthesis genes? (1992)
    New York, NY : Wiley-Blackwell
    BioEssays 14 (1992), S. 823-830 
    Details
    ISSN: 0265-9247
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: After yeast cells commit to the cell cycle in a process called START, genes required for DNA synthesis are expressed in late G1. Periodicity is mediated by a hexameric sequence, known as a MCB element, present in all DNA synthesis gene promoters. A complex that specifically binds MCBs has been identified. One polypeptide in the MCB complex is Swi6, a transcription factor that together with Swi4 also binds G1 cyclin promoters and participates in a positive feedback loop at START. The finding that Swi6 is directly involved in both START and DNA synthesis gene control suggest a model in which Swi6, activated through its participation in START, serves as the central transcription factor in coordinating late G1 gene expression. The mechanism may be conserved in all eukaryotic cells.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bies.950141206
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