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  • 1
    Electronic Resource
    Electronic Resource
    Ir GENE TESTING IN H-2 MUTANTS (1980)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 7 (1980), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Four different C57BL/6 mutants, each with a mutation presumably in the H-2Kb locus, were studied to determine whether the mutations had affected Ir gene function. When tested with the antigens, (TG)-A-L, ovalbumin (OA), and lactic dehydrogenase (LDH8), there was no difference in the antibody response of the mutants when compared to the parental C57BL/6 strain and there was also no difference in the response to the histocompatibility H-Y antigen. The responses to these antigens have been mapped, wholly or partially in the K-IA, IA or IB subregions and therefore the identical response of parent and mutants may provisionally exclude H-2Kb as a site of Ir gene function for the four antigens tested. In addition, the BALB/c-dm2 mutant, a mutation in the H-2Ld locus, gave identical responses to the parental BALB/cKh strain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.1980.tb00736.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The definition of a new IA antigenic specificity using the B6.C-H-2 bm12 I-region mutant strain (1980)
    Springer
    Immunogenetics 11 (1980), S. 1-6 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A new Ia specificity has been defined using theIA-subregion mutant B6.C-H-2 bm12. The immunization to produce the antiserum wasbm12 anti-A.BY, as all other immunizations, such asbm12 anti-C57BL/6, failed to produce antibody. By selecting strains of C57BL origin for testing, it was shown that, (a) the serum was only weakly cytotoxic but gave substantial reactions using a rosetting assay; (b) the antibody reacted with B cells and not T cells; (c) strains of theb, d, p andq H−2 haplotypes were positive, whereasf, k, r ands were negative; (d) absorption studies demonstrated only a single specificity to be present and by testing recombinant strains, the reaction mapped to theIA subregion; (e) SDS-PAGE demonstrated that the antiserum reacted with a molecule of MW ∼33 000. Preliminary studies indicate this new specificity, present on C57BL/6 and lost frombm12, is present on the same molecule as other I-A specificities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01567764
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Studies of twoH-2D b mutants: B6.C-H-2 bm13 and B6.C-H-2 bm14 (1980)
    Springer
    Immunogenetics 11 (1980), S. 341-349 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Two new C57BL/6H-2 mutants,B6.C-H- 2bm13 and B6.C-H- 2bm14 are described. They arose independently in C57BL/6 as spontaneous mutations of the gain and loss type. Complementation studies map the mutations in both bm13 and bm14 to theH-2D b gene. How ever, these two mutant strains are not identical, but occurred as independent mutations at the same locus, as shown by reciprocal graft rejection and by the inability of the (bm13 × bm114)F1 hybrid to accept C57BL/6 grafts. Serological studies by direct testing (cytotoxicity and hemagglutination) and by quantitative absorption demonstrated a decrease in the H-2Db private specificity H-2.2 in both bm13 and bm14 when compared to C57BL/6. This was confirmed by SDS-PAGE analysis using antisera detecting the H-2.2 specificity. Attempts to produce antibodies to either the gained or lost specificities of the two mutant strains failed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01567801
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    Paper (German National Licenses)
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