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  • 1
    Electronic Resource
    Electronic Resource
    Stop-motion illuminator for centrifuges (1999)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 70 (1999), S. 4433-4434 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: The majority of laboratory centrifuges have no provisions for observation of the process of sedimentation and fractionation which goes on in the test tube in the darkness of a "black box." Some centrifuges have a transparent cover or a magnifier in the cover to watch a blurred picture of the layers in the moving test tube which provides little information. The stop-motion effect with the test tube can a priori be based on either mechanical or electronic stroboscopy. To implement the mechanical stroboscopy, a standard microscope illuminator with a diaphragm is attached to the wall of the centrifuge. Through a hole in the wall the illuminator projects a cone of light with the diameter 0.1 mm at the top of the cone. The cone intersects with a 0.1 mm hole in the test-tube holder. The test tube is illuminated at the moment of intersection. Its content is observed through a magnifier on the cover of the centrifuge. The holder has a cut-out window to see the test tube. The flash at the hole–beam intersection is 0.5 μs, which is many times shorter than the duration of a xenon-tube stroboscopic flash (10–20 μs). This is the main reason why industrial stroboscopes cannot be used for stop-motion illumination in a centrifuge. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1150089
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  • 2
    Electronic Resource
    Electronic Resource
    Prospects of centrifugation of cells, liquids, and gases at multimillion g-forces in magnetically suspended microrotors (1986)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 57 (1986), S. 598-601 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: Prototypes of magnetically suspended rotors for centrifugation of cells, organic and inorganic solutions, and gases are Fe-containing glass and metallic hollow microspheres 0.1–2 mm in diameter, composite steel-glass disks up to 1 cm, and microspheres of porous steel and Fe-impregnated porous glass. Fe-coated microcapsules with cells can be used for lower centrifugal forces. Predictable applications include cell hybridization and homogenization, spinning of samples at the "magic angle'' in nuclear-magnetic resonance (NMR), intensification of nuclear fusion by high-speed rotation of microspheres with deuterium and tritium, and other examples. Rotor contents can be examined under the microscope during centrifugation, or the spinning rotor can be frozen and its contents freeze dried for further examination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1138876
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  • 3
    Electronic Resource
    Electronic Resource
    A hypothesis of the code of nerve impulses (1980)
    Springer
    Acta biotheoretica 29 (1980), S. 101-109 
    Details
    ISSN: 1572-8358
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract There is probably only one information system in living nature — the macromolecular system including DNA, RNA and protein. Its unity for the genetic and nervous activity can be followed in the storage of information (heredity, memory) and in its processing (recombination and selection of both genetic and mental information). According to the hypothesis of the code of nerve impulses, nucleotide triplets of the nucleus, or more likely amino acids of the surface protein of the impulse generating area of a neuron, generate a limited variety of interspike intervals so that each amino acid corresponds to a certain interspike interval and this particular interval initiates by means of a specific neurotransmitter, the synthesis of the same amino acid (or nucleotide triplet) in the postsynaptic neuron. Thus, a series of impulses produces in the postsynaptic neuron a sequence of amino acids in a form of a polypeptide identical to the polypeptide of the presynaptic neuron.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00046375
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  • 4
    Electronic Resource
    Electronic Resource
    Centrifugal cell hybridization (1984)
    Springer
    Journal of biological physics 12 (1984), S. 17-20 
    Details
    ISSN: 1573-0689
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract Centrifugation of cell suspensions containing two or several cell types at forces up to 2 million g results in several basic events in succession: 1. Intracellular stratification. 2. Extrusion of the most dense cell parts (nuclei or cytoplasmic components) and their penetration into an adjacent cell in the compact sediment. Such introduction of protoplasmic elements from one cell into another is considered as centrifugal hybridization and fusion of cells. It differs from other methods of cell hybridization by its selectivity for cell components. 3. Further intermingling and mixing of cells into a fused protoplasmic mass. 4. With continuing increase of centrifugal force fractions of subcellular components are formed from the protoplasmic mass. These components are presumably viable since cells are not exposed to chemical treatment. Morphological demonstration of hybridization is based on centrifuging microscopy and on labeling donor cells or recipient cells by staining or fluorescence. Genetic evidence can be provided by cultivation of hybrid cellsin vitro and their cloningin vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01857617
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