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Demineralization with EDTA by constant replacement (1965)

Bélanger, Leonard F. ; Copp, D. Harold ; Morton, Margaret A.

New York, NY [u.a.] : Wiley-Blackwell

The @Anatomical Record 153 (1965), S. 41-47

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ISSN: 0003-276X

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Medicine

Notes: An apparatus for demineralization of hard tissues by constant replacement of a saturated solution of EDTA is described. This method allows for more rapid demineralization through better Ca chelation. EDTA demineralization preserves cells and matrix constituents particularly the mucopolysaccharides as demonstrated by staining, Alpharadiography, phase contrast microscopy and microincineration. Cold acetone fixation followed by cold EDTA demineralization retains several enzymic activities.

Additional Material: 1 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/ar.1091530106

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