ISSN:
1040-0397
Keywords:
Biosensor
;
Alcohol
;
Avidin
;
Biotin
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Protein multilayers composed of avidin and biotin-labeled alcohol oxidase (AOx) were prepared on the surface of a platinum (Pt) electrode by repeated deposition of the proteins to develop amperometric alcohol sensors. The AOx multilayer-modified sensors showed a rapid response to ethanol over the concentration range of 0.01-10mg/100mL. The output current of the sensor depended linearly upon the thickness of the AOx layers (or the number of the AOx layers), suggesting that a constant amount of AOx can be immobilized by each deposition. The selectivity of the sensor to aliphatic alcohols was in the order of methanol 〉 ethanol 〉 n-propanol 〉 n-butanol, being in accord with the selectivity of AOx in a homogeneous system itself. The sensor retained its original response to ethanol for about one month without deterioration.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140080820
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