Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Molecular Identification of the 62 kd Form of Glutamic Acid Decarboxylase from the Mouse (1990)
    Oxford, UK : Blackwell Publishing Ltd
    European journal of neuroscience 2 (1990), S. 0 
    Details
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A series of overlapping clones coding for L-glutamic acid decarboxylase was purified from a mouse brain cDNA library, the longest of which contains a 1869 bp open reading frame and 913 bp of non-coding sequence. By comparison with the corresponding sequences from the mouse genome, it was determined that the first methionine in the longest cDNA represents the initiation codon. Expression of this cDNA in eukaryotic cells produces a 62 kd protein that is recognized by antiserum against rat GAD and which displays GAD activity commensurate with the amount of protein produced. Antibodies raised against the purified product of this cDNA recognize a 62 kd protein from mouse brain on immunoblots, specifically stain GABA-ergic neurons in brain sections, and are capable of immunoprecipitating most GAD activity from mouse brain extracts. These results provide the first definitive identification of a cDNA coding for the larger of two forms of GAD in mouse brain, and suggest that the two forms are closely related.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1460-9568.1990.tb00412.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Structure of Flavoglaucin and Auroglaucin (1949)
    [s.l.] : Nature Publishing Group
    Nature 164 (1949), S. 26-27 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] FLAVOGLAUCIN, C19H28O3, m.p. 109–110°, and auroglaucin, C19H22O3, m.p. 152–153°, are two pigments, yellow and red respectively, isolated by Raistrick and his collaborators1 from Aspergillus glaucus, and later found ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/164026a0
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Filamentous inclusions in the matrix of mitochondria from human livers
    Amsterdam : Elsevier
    Journal of Ultrasructure Research 11 (1964), S. 525-544 
    Details
    ISSN: 0022-5320
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0022-5320(64)80080-0
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Distribution of Cholecystokinin-like Immunoreactivity in the Brainstem Auditory System (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 448 (1985), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1985.tb29949.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Observations on the fine structure of the lateral vestibular nucleus (Deiters' nucleus) in the cat (1967)
    Springer
    Experimental brain research 4 (1967), S. 146-186 
    Details
    ISSN: 1432-1106
    Keywords: Deiters' nucleus ; Ultrastructure ; Synaptology ; Satellitosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A study has been made of the ultrastructure of the lateral vestibular nucleus of the normal cat. The study includes light microscopical observations made in Golgi material. The internal structure of the various types of cells is described. The soma of the larger nerve cells is surrounded by a protoplasmic layer, constituted by astroglial sheets, dendrites and boutons; glial cell bodies are usually located outside the layer. The smaller nerve cells display few axosomatic synapses and may be in direct contact with myelinated fibres and glial perikarya. Spines are present on the soma of large and small nerve cells and on all parts of the dendrites. The proximal dendrites are usually the part of the neurons which is most amply supplied with boutons. Various types of boutons and cell junctions are described and an attempt is made to correlate the findings in electron micrographs with those made in Golgi sections. The study serves as a basis for observations made in experimental material where afferent fibres to the nucleus have been damaged.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00240360
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Mode of termination of primary vestibular fibres in the lateral vestibular nucleus an experimental electron microscopical study in the cat (1967)
    Springer
    Experimental brain research 4 (1967), S. 187-211 
    Details
    ISSN: 1432-1106
    Keywords: Deiters' nucleus ; Electron microscopy ; Primary fibres ; Operated cats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Following transection of the vestibular nerve in cats, the electron microscopical changes occurring in the lateral vestibular nucleus were studied after survival periods of 2–11 days. Material for study was taken from the rostroventral part of the nucleus of Deiters since this is known to receive the primary vestibular fibres. Degeneration of terminal boutons is evident two days after the lesion. Degenerating boutons show an increased electron optic density, mitochondrial changes and a loss of synaptic vesicles. They are often surrounded by a pericellular space filled with flocculent (probably protein) material. At three days and later this space is occupied by processes of astrocytes or of a type of phagocytic cells which surround or engulf the degenerating boutons. Nine to eleven days after the lesion almost all degenerating boutons have disappeared. There is evidence of phagocytosis of axons and myelin sheaths by astrocytes but mainly by phagocytes of yet undetermined origin. The “filamentous type” of bouton degeneration has not been observed. Degenerating boutons are found on neuronal perikarya and on proximal as well as on thin distal dendrites and on spines. They are common on small and medium-sized cells, but have also been seen on some giant cells. The degenerating boutons do not form series of synaptic complexes. Degenerating fibres and boutons have so far been found only ipsilateral to the lesion. The findings confirm and extend those made in corresponding experiments with silver impregnation procedures, but emphasize the limitations of the latter methods as regards conclusions concerning synaptic contacts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00248022
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    An experimental electron microscopical study on the mode of termination of cerebellar corticovestibular fibres in the cat lateral vestibular nucleus (Deiters' nucleus) (1967)
    Springer
    Experimental brain research 4 (1967), S. 212-236 
    Details
    ISSN: 1432-1106
    Keywords: Deiters' nucleus ; Electron microscopy ; Purkinje axons ; Operated cats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The present paper is an experimental study on the mode of termination of cerebellar corticovestibular fibres in the cat. The distribution of degenerating terminal structures as this appears in electron micrographs of eight animals with a survival time from three up to eleven days is described. The early stage of degeneration of Purkinje cell axons is a filamentous reaction during which fibres as well as boutons are enlarged and filled with filaments. The initial reaction is followed by shrinkage, and many fibres and boutons are at the 4 day stage electron dense, and no filaments can be recognized. Other fibres and boutons show intermediate stages of degeneration. Only dark degenerating axons and boutons are present at the 11 day stage. The observations are related to those made in other nuclei and regions where degeneration has been described in electron micrographs. Degenerating terminal myelinated fibres ending with terminal and en passage boutons are found. An attempt is made to correlate the findings with those made in normal animals and in Golgi sections. The mode of termination and the pattern of branching of Purkinje cell axons are discussed. The degenerating terminal structures are in synaptic contact with cells of all sizes and with all parts of the neurons, i. e., soma, proximal and distal dendritic trunks and spine-like projections. Elongated and rounded synaptic vesicles are present in the degenerating boutons. The glial reaction adjacent to degenerating boutons is also described, and brief mentioning is made of findings in the intracerebellar nuclei of the same animals. The findings in these nuclei are essentially the same as those made in the lateral vestibular nucleus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00248023
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Distribution and dendritic features of three groups of rat olivocochlear neurons (1992)
    Springer
    Anatomy and embryology 185 (1992), S. 1-16 
    Details
    ISSN: 1432-0568
    Keywords: LOCS ; MOCS ; Auditory brain stem ; Superior olivary complex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cholera toxin B subunit conjugated to horseradish peroxidase, and unconjugated cholera toxin B subunit are useful tools for retrograde tract tracing. Unilateral injection of either cholera toxin preparation into the cochlea results in excellent labeling of olivocochlear neurons, as judged by the Golgi-like filling of cell bodies, dendrites, and even axons. By this approach, we have studied the light microscopic cytology and topographic distribution of olivocochlear neurons and counted their numbers in Sprague-Dawley rats. The olivocochlear system of rats can be divided into three subgroups. The lateral olivocochlear system, composed of small cells located exclusively within the ipsilateral lateral superior olive (relative to the test cochlea), and a medial olivocochlear system, composed of large cells bilaterally dispersed within the ventral nucleus of the trapezoid body, conformed to previous topographic descriptions. A third subgroup of approximately 110 large cells, herein termed shell neurons, was labeled by both tracers, but was not well recognized in previous studies. Shell neurons and their dendrites surround the ipsilateral, and to a much lesser extent the contralateral, lateral superior olive. Lateral olivocochlear neurons do not project their dendrites outside the gray matter of the lateral superior olive, while dendrites belonging to shell neurons penetrate into that nucleus as well as into other auditory brain stem nuclei and the surrounding reticular formation. Medial olivocochlear neurons usually project dendrites ventrally into the trapezoid body and are always excluded from the lateral superior olive.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213596
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Development and fine structure of murine Purkinje cells in dissociated cerebellar cultures: dendritic differentiation, synaptic maturation, and formation of cell-class specific features (1997)
    Springer
    Anatomy and embryology 197 (1997), S. 31-50 
    Details
    ISSN: 1432-0568
    Keywords: Key words Purkinje neuron ; Synaptogenesis ; Dendritogenesis ; L7 ; Cerebellum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The morphological differentiation of E16 murine Purkinje cells (PCs) in dissociated cerebellar cultures was analyzed by light and electron microscopic immunocytochemistry after 2–5 weeks in vitro (wiv), with particular emphasis on dendritic differentiation, synaptic maturation, and formation of stereotypical fine structural features. This study complements a companion paper on the features of PCs after 1 wiv. After 2 wiv, the PCs have an eccentric nucleus and the cytoplasmic organelles appear immature; the axon has a distinct initial segment and beaded axon collaterals but its boutons still contain sparse synaptic vesicles; dendrites show few bifurcations and tufts of spiny branchlets. After 3 wiv, the PCs display a centered nucleus, an extensive hypolemmal cisternal system, and stacks of up to four cisterns of granular endoplasmic reticulum; there is an increased number of dendritic bifurcations, spiny branchlets, mature spines, and axonal branches; dendritic tips still contain vesicle clusters, suggesting growth, and many synapses and afferent boutons continue to display immature features. After 4 wiv, elaborate perinucleolar coiled body rosettes, subsurface cistern-mitochondrion complexes and large stacks of granular endoplasmic reticulum finally appear within the soma; dendrites show a further increase in the numbers of bifurcations, segments and spines; most spines are synaptic and show mature features; afferent synapses are differentially distributed; PC boutons consistently display mature features and show a considerable degree of target specificity, although naked spines and reduced glial sheaths persist. After 5 wiv, PCs do not show further maturation and some dystrophic features appear. We conclude that under standard conditions and despite the disruption of normal tissue organization, PCs in dissociated cultures differentiate maximally after 4 wiv, at which stage they display many of the light and electron microscopic features that characterize mature PCs in situ. This prolonged developmental time-frame resembles that in the normal cerebellum. In view of the increasing usage of dissociated cerebellar cultures to study aspects of neuronal differentiation, synaptic activation and neuronal-glial interactions, an elucidation of the neurocytology of dissociated cerebellar cultures as presented in this study provides important clues for the interpretation of experimental data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004290050118
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Development and fine structure of murine Purkinje cells in dissociated cerebellar cultures: neuronal polarity (1997)
    Springer
    Anatomy and embryology 197 (1997), S. 9-29 
    Details
    ISSN: 1432-0568
    Keywords: Key words Cerebellum ; Dendritogenesis ; Filopodia ; Growth cone ; L7 ; Polarization ; Purkinje neuron ; Synaptogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Cerebellar Purkinje cells (PC) display a highly distinctive form of polarity. We have cultured murine PCs from dissociated E16 cerebellar anlagen for 1 week to investigate the early stages of neuronal compartmentalization and synaptic interactions, features which are important for the establishment of neuronal polarity. To unequivocally identify the PCs we utilized light and electron microscopic immunocytochemistry with an antiserum to the cell class-specific marker L7/pcp2 gene product. The PCs typically show a single, long axon, numerous short appendages classified as filopodia and protospines, and a small number of protodendrites. The nucleus is positioned asymmetrically in both the horizontal and vertical axes of the soma. The Golgi apparatus, coated and uncoated vesicles, and mitochondria are prominent ultrastructural features, while the endoplasmic reticulum is highly fragmented. The cell body receives rudimentary synapses on its smooth surfaces and appendages and no consistent morphological differences were detected between these elementary contacts. The axon is clearly identifiable; it emanates from either the cell body or a protodendrite, bifurcates at predominantly right angles, forms beaded collaterals, and terminates with relatively large growth cones. The varicosities of the PC axon contain pleomorphic synaptic vesicles and form rudimentary synapses primarily with the dendritic shafts of immunonegative neurons. The protodendrites are short, quickly tapering and sparsely branched; they emit numerous filopodia and immature spines and terminate with small growth cones. Rudimentary synapses are received on the proximal dendritic shafts and filopodia, and more mature synapses occur frequently on protospines. With few exceptions, PCs lie atop an astrocytic bed layer and glial processes are apposed to the various aspects of the PC body left free by the afferent axons. By contrast, PC processes are largely free of glial sheaths. We conclude that the ”stellate stage” of PC development in situ is replicated rather faithfully in culture and that PCs have established polarity and have begun to form intercellular contacts by 1 week in vitro. Moreover, the PCs are already morphologically distinct from other cell types in the 1-week cultures, although they have yet to develop the differentiated features that distinguish mature PCs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004290050117
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...