ISSN:
0886-1544
Keywords:
inhibition of cell motility and proliferation by interferon-β
;
interferon-β increases stationary time in fibroblasts
;
interferon-β decreases translocation rate in fibroblasts
;
fibroblast motility in culture
;
cell motility: translocation rate and stationary time
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
The rate of translocation and the percent of the time that cells are stationary have been measured by computer-assisted time-lapse cinemicrography in over 1,000 freshly planted human foreskin fibroblasts (FS-4 cell strain) for periods of up to a week and the effects of interferon-β (IFN-β) on these parameters have been determined. Cells were planted at 2.5 × 103 cells/cm2 in Eagle's minimal essential-medium (MEM) with 10% fetal bovine serum (FBS). Frames were taken every 2 or 4 minutes and data were collected on both cell location and cell division as a function of time. After planting FS-4 cells require ∼48 hr to reach maximum motility both with respect to the translocation rate when moving and percent time cells are moving. Recombinant human IFN-β (800 μ/ml) caused a marked increase in the fraction of time cells were stationary and a decrease of lesser magnitude in the translocation rate, as quantitated during the period during which the stationary fraction for control cells was at a minimum. IFN-β also decreased the rate of cell proliferation, without any evidence of degeneration or death of cells. Our results contribute new evidence that the fraction of time cells spend moving directionally is an important determinant of their locomotory behavior and that this determinant is responsive to modulation by cytokines.
Additional Material:
12 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/cm.970190205
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