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  • 1
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Studies were performed to measure the protein quality of spray-dried whole milk and of casein using a short-term nitrogen balance index with adult human subjects. Comparative values were obtained using the conventional nitrogen balance assay technique. The quality of the protein was calculated by regression analysis of all nitrogen absorbed to nitrogen retained. The coefficients of regression for milk when the short-term approach was used were 1.06, 0.97, 0.91 and 0.88. The differences were not statistically significant. With the conventional method, the value obtained was 0.88. The value from the conventional method was not statistically different from those obtained by. the short-term variation, which has a tendency to give higher values. The coefficient of regression of nitrogen absorbed to nitrogen retained for casein was 0.65. The results also suggested less variation if the nitrogen balances per protein intake are of two days duration, allowing for more representative fecal collections. The results obtained suggest the short-term nitrogen balance index to be a suitable approach to protein quality evaluation in humans, although we recognize the need to perform additional studies to standardize the technique as much as possible.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The rice bean (Phaseolus calcaratus), cowpea (Vigna sinensis), red bean, and three black bean (Phaseolus vulgaris) samples grown in Guatemala, were found to be fairly similar in chemical composition. Average percentage values were: moisture 14.5, protein 22.3, ether extract 1.3, ash 3.5, crude fiber 6.0, and carbohydrate 52.4. The range in amino acid content, expressed as mg of amino acid per gram of nitrogen, was: arginine 356-528, histidine 159-232, isoleucine 143-339, leucine 189-260, lysine 322-544, total sulfur-containing amino acids 80-94, phenylalanine plus tyrosine 362-471, tryptophan 52-73, threonine 215-348, and valine 191-383. The cowpea sample was higher in lysine; the rice bean and red bean samples were higher in tryptophan. The isoleucine and valine contents were lower, and the leucine higher, in the red beans than in the other samples. When compared with the amino acid pattern of the FAO Reference Protein, methionine plus cystine was the most-limiting amino acid, and leucine and tryptophan respectively the second- and third-most limiting. All the samples contained high amounts of lysine, making beans a good source of this amino acid.
    Type of Medium: Electronic Resource
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