ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract: For a study of the localization of various forms of the γ subunit of G proteins, antibodies were raised in rabbits against peptides that corresponded to partial amino acid sequences of bovine γ2, γ3, γ5, and γ7. Affinity-purified antibodies against γ2, γ3, and γ5 reacted specifically with γ2, γ3, and γ5, respectively, but the antibody against γ7 reacted with γ2, γ3, and a novel γ subunit, designated γs1, as well as with γ7. Because these antibodies reacted with the respective forms of the γ subunit from rat brain, we investigated the localization of γ subunits in the rat. γ2 and γ3 were abundant in all regions in the brain, whereas the concentration of γ5 and γ7 was relatively low with the single exception being a high concentration of γ7 in the striatum. The concentration of γ2 was consistently high during ontogenic development in the rat brain, whereas γ3 appeared about a week after birth and their concentrations then increased until a month after birth. In tissues other than the brain, γ3 was observed only in the pituitary gland, whereas γ2, γ5, and γ7 were found in a variety of tissues. In addition, most tissues contained relatively high concentrations of some other γ subunit, which was detected with an antibody against a γ7-derived peptide and appeared to be γs1. Among cloned cells tested, γ3 was detected only in PC12 pheochromocytoma cells. Taken together, the results indicated that γ3 was expressed specifically in neuronal cells, and γs1 was the major γ subunit in most nonneural cells. γ2, γ5, and γ7 were distributed in a variety of tissues, but γ2 was dominant in the brain.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1471-4159.1995.64031267.x
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