ISSN:
1573-7373
Keywords:
RLGS
;
gel electrophoresis
;
genetic subtypes
;
glioblastoma
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The 16 primary gliomas were examined for changes in genomic DNA using arecently developed 2-dimensional gel electrophoresis method calledrestriction landmark genomic scanning (RLGS). This approach allows detectionof DNA amplification, deletion, methylation and potentially other geneticrearrangements represented as decreases and increases in spot/fragmentintensity on an autoradiogram. Approximately 2,000 landmark sites in tumorDNA were compared with those of DNA isolated from normal brain tissues.Seven spots showing intensified signal were consistently detected in atleast 50% of tumors, implying activation of corresponding DNAsequences, and 8 additional spots having reduced signal were observed, againin more than 50% of all tumors, suggesting inactivation by the lossof 1 allele or homozygous deletion. Decreased signal may also infer relativeCpG island methylation state. Of those spots consistently identified intumors, 2 amplified and 4 reduced spots were found to be characteristic oflow- and high-grade tumors, while the remaining 5 amplified and 4 reducedspots were associated with high-grade gliomas only, suggesting a link ofspecific mutations to degree of malignancy. A separate subset ofglioblastomas evaluated, however, showed no alterations in these ’hot spots‘which were detected in even low grade astrocytomas. The results demonstratethe genetic heterogeneity of glioblastoma and implicate the progression ofneoplasia via differing genetic pathways.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005712308061
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