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  • 1
    Electronic Resource
    Electronic Resource
    A simplified method for determination of tritiated thymidine incorporation into cells from tissue in soft agar culture (1986)
    Springer
    Surgery today 16 (1986), S. 235-238 
    Details
    ISSN: 1436-2813
    Keywords: tritiated thymidine incorporation ; soft agar culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We designed a simple method forin vitro chemosensitivity testing of tritiated thymidine incorporation by cells from tumor tissue plated in soft agar. Cells from tumor tissue were cultured in a plastic dish, of which a Millipore membrane had been sealed to the underside, containing two layers of agar. After labeling the cells with tritiated thymidine, the dishes were placed in a boiling water bath to solubilize the agar. The labeled cells could be readily collected on the membrane by evacuating the solubilized medium through membrane filters sealed underside of the dish. Using this system, 11 (55 per cent) of 20 tumor specimens from 20 patients with various carcinomas showed an incorporation of over 200 dpm above background and 80 per cent or greater inhibition of thymidine uptake by sodium azide treatment. This method offered several advantages over the thymidine incorporation assay, including technical simplicity and a shorter time course.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02471099
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Colony growth of cells from primary breast carcinoma in soft agar culture (1984)
    Springer
    Surgery today 14 (1984), S. 212-216 
    Details
    ISSN: 1436-2813
    Keywords: breast carcinoma ; colony growth ; soft agar ; clinical stage ; sensitivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract An in vitro soft agar culture system was utilized to evaluate the colony growth of cells from primary breast carcinoma. A total of 53 specimens from fifty-three patients were placed in culture. Of these, 29 samples (55 per cent) formed at least 30 colonies per 500,000 cells plated. In relation to histologic type of tumor and clinical status of the disease, 4 of 4 samples from mucous carcinoma grew into colonies and, then, t-categories, i.e. histological extent of primary tumor, and colony growth showed an inverse correlation. Estrogen receptor status did not appear to influence growth of the colonies. The in vitro sensitivity studies to adriamycin showed a dose dependent increase in lethality. However, the in vitro response rate was relatively low. This assay system can be used to study the biology and clinical approaches to treatment of breast carcinoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02469570
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Cellular hypersensitivity to autologous tumor extract in patients with breast carcinoma (1978)
    Springer
    Surgery today 8 (1978), S. 236-241 
    Details
    ISSN: 1436-2813
    Keywords: cellular immunity ; indirect migration inhibition test ; breast carcinoma ; TNM classification ; survival rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The indirect macrophage migration inhibition technique was used to study cellular hypersensitivity to autologous tumor extract in relation to the progress and prognosis of breast carcinoma. Cellular immune response, evidenced by production of a macrophage migration inhibitory factor (MIF), was noted preoperatively in 21 of 63 patients (33 per cent). This reactivity was used at the time of surgery to determine the grade of the primary tumor, lymph node involvement and the stage of the disease according to the TNM system. The five-year survival rate was 76 per cent for patients whose lymphocytes responded preoperatively and 54 per cent for patients whose lymphocytes did not respond, indicating that this assay may be valuable in detecting cellular immune response to breast carcinoma and in evaluating the immunological status of patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02469449
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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