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  • 1
    Electronic Resource
    Electronic Resource
    The same nuclear proteins bind to the 5′-flanking regions of genes for the rice seed storage protein: 16 kDa albumin, 13 kDa prolamin and type II glutelin (1996)
    Springer
    Plant molecular biology 32 (1996), S. 621-630 
    Details
    ISSN: 1573-5028
    Keywords: rice seed storage protein ; albumin ; gene expression ; glutelin ; prolamin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Expression of rice seed storage-protein genes is dramatically regulated over a short period of seed maturation. To characterize the expression mechanism of the rice seed storage protein genes, their expression of major storage protein genes (16 kDa albumin, 13 kDa prolamin and type II glutelin) were compared by RNA blot analysis. Their coordinate expression suggests that the transcriptional regulatory machinery is shared among the glutelin, prolamin and albumin-genes. We isolated two novel genomic genes for prolamins (PG5a and PG5b) and obtained the promoter region of the glutelin gene by PCR. The 5′-flanking regions of these three rice seed storage protein genes were found to contain some similar conserved sequences. Nuclear extract partially purified from maturing rice seeds was used for the gel shift assay of the 5′ region of the RA gene. We identified two DNA sequences of RA gene which were recognized by independent DNA-binding proteins. The complexes of these DNA sequences and DNA-binding proteins were inhibited by the fragments containing the 5′ regions of the prolamin and glutelin genes, suggesting that these three genes share transcription factors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020203
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of a novel rice bZIP protein which binds to the α-globulin promoter (1997)
    Springer
    Plant molecular biology 33 (1997), S. 513-522 
    Details
    ISSN: 1573-5028
    Keywords: allergen ; bZIP ; α-globulin ; rice ; storage protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Many plant basic leucine-zipper (bZIP) proteins have been isolated several of which have been shown to play a role in seed-specific gene expression. We isolated a novel bZIP protein (REB) gene encoding 425 amino acid residues from rice endosperm, which is similar to Opaque-2 heterodimerizing protein (OHP) of maize. The gene product, termed REB, contains Pro- and Gly-rich regions at its N terminus, followed by the typical basic and leucine-repeat regions. Recombinant REB binds to the region from -754 to -562 in the α-globulin gene promoter, but not to promoters of other major storage genes such as glutelin, prolamin and albumin. The 5′ region of the α-globulin gene possesses three binding sites for REB, which were determined as GCCACGT(A/C)AG, by using synthetic oligonucleotides. A Super-shift assay using anti-REB antibody suggested that REB is a major DNA-binding protein for the α-globulin gene promoter in rice endosperm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005784717782
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    Paper (German National Licenses)
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