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  • 1
    Electronic Resource
    Electronic Resource
    Heterogeneity of Protein Profiles of Helicobacter pylori Isolated from Individual Patients (1998)
    Cambridge, MA, USA : Blackwell Science Inc, Boston, USA
    Helicobacter 3 (1998), S. 0 
    Details
    ISSN: 1523-5378
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In order to characterize the diversity of Helicobacter pylori (H. pylori) in infected individuals, 10 colonies of H. pylori were isolated from the gastric juice of 25 patients with gastroduodenal diseases (total 250 isolates).〈section xml:id="abs1-2"〉〈title type="main"〉Methods.Protein profiles of isolates were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Results were confirmed by Western blotting (immunoblotting) test using rabbit antisera against three different strains of H. pylori.〈section xml:id="abs1-3"〉〈title type="main"〉Results.The protein profiles of 18 of 25 cases (72%) showed a single type of H. pylori with the same polypeptide pattern. In contrast, heterogeneity in the protein profiles was seen in isolates from seven cases (28%). Two differing H. pylori types with two very different polypeptide patterns were found in 10 isolates from one case. In six patients, the protein profiles of isolates were found to have variations in their polypeptides between molecular weights of 30,000 (30K) and 14K, which are thought to be associated with bacterial membrane protein. In some isolates, a polypeptide band of the 16K was missing. Each of three different antisera confirmed differences among the distinct isolates from individual patients.〈section xml:id="abs1-4"〉〈title type="main"〉Conclusions.These findings suggest that more than one antigenically different strain of H. pylori may exist in same infected individuals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1523-5378.1998.08703.x
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  • 2
    Electronic Resource
    Electronic Resource
    Serum antibody againstHelicobacter pylori assayed by a new capture ELISA (1995)
    Springer
    Journal of gastroenterology 30 (1995), S. 295-300 
    Details
    ISSN: 1435-5922
    Keywords: Helicobacter pylori ; enzyme-linked immunosorbent assay ; monoclonal antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We developed a highly specific detection technique for serum antibody, using a monoclonal antibody to a specific antigen ofHelicobacter pylori. A monoclonal antibody preparation that reacted with the 54-kDa molecule ofH. pylori antigens was obtained. Using this preparation, an antigen-capture enzymelinked immunosorbent assay (ELISA) was established by fixation of the monoclonal antibody, followed by reaction with sonicated whole cell antigens. The serum antibody titers of patients with gastritis and peptic ulcers were significantly higher than control titers, and the antibody titer correlated with the histological severity of gastritis. Patients positive forH. pylori by bacterial culture had higher titers thanH. pylori-negative patients. Our new ELISA may be useful for the diagnosis ofH. pylori infections and for evaluation of the severity of gastric inflammation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02347502
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    Paper (German National Licenses)
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