ISSN:
1573-5028
Keywords:
cDNA
;
gametophytic self-incompatibility
;
Lycopersicon peruvianum
;
polymerase chain reaction (PCR)
;
ribonuclease activity
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We isolated S allele-associated cDNA clones from each of the stylar cDNA libraries of Lycopersicon peruvianum of two different S genotypes (S 12Sband S 13 S c) with S 11 S callele-associated cDNA (LPS11) as a probe. The longest cDNA clones, designated LPS12 and LPS13, which were 779 bp and 853 bp in length, contained open reading frames of 189 and 210 amino acids, respectively. The three S alleleassociated cDNAs (LPS11, LPS12, and LPS13) did not cross-hybridize to each other under highly stringent condition by northern blot analysis. Their average identity to Nicotiana alata S-proteins so far was 49%. The fragments corresponding to LPS11 or LPS12 cosegregated with their respective S alleles in genetic crosses. From these results, we conclude that the three cloned cDNAs were derived from the three different S alleles of L. peruvianum.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00013760
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