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  • 1
    Electronic Resource
    Electronic Resource
    Somatic hybrid plants between the forage legumes Medicago sativa L. and Medicago arborea L. (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 183-189 
    Details
    ISSN: 1432-2242
    Keywords: Key wordsM. sativa ; M. arborea ; Protoplast electro-fusion ; Somatic hybrids ; Genome rearrangements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Interspecific somatic hybrid plants were obtained by symmetrical electrofusion of mesophyll protoplasts of Medicago sativa with callus protoplasts of Medicago arborea. Somatic hybrid calli were picked manually from semi-solid culture medium after they were identified by their dual color in fluorescent light. Twelve putative hybrid calli were selected and one of them regenerated plants. The morphogenesis of the somatic hybrid calli was induced by the synthetic growth regulator 1,2 benzisoxazole-3-acetic acid. Somatic hybrid plants showed intensive genome rearrangements, as evidenced by isozyme and RFLP analysis. The morphology of somatic hybrid plants was in general intermediate between the parents. The production of hybrids by protoplast fusion between sexually incompatible Medicago species is related to the in vitro responsiveness of the parental protoplasts. The possibility of using somatic hybrid plants in alfalfa breeding is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050264
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Somatic hybrid plants between the forage legumes Medicago sativa L. and Medicago arborea L. (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 183-189 
    Details
    ISSN: 1432-2242
    Keywords: M. sativa ; M. arborea ; Protoplast electrofusion ; Somatic hybrids ; Genome rearrangements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Interspecific somatic hybrid plants were obtained by symmetrical electrofusion of mesophyll protoplasts of Medicago sativa with callus protoplasts of Medicago arborea. Somatic hybrid calli were picked manually from semi-solid culture medium after they were identified by their dual color in fluorescent light. Twelve putative hybrid calli were selected and one of them regenerated plants. The morphogenesis of the somatic hybrid calli was induced by the synthetic growth regulator 1,2 benzisoxazole-3-acetic acid. Somatic hybrid plants showed intensive genome rearrangements, as evidenced by isozyme and RFLP analysis. The morphology of somatic hybrid plants was in general intermediate between the parents. The production of hybrids by protoplast fusion between sexually incompatible Medicago species is related to the in vitro respon siveness of the parental protoplasts. The possibility of using somatic hybrid plants in alfalfa breeding is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225744
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Chromosomal and molecular rearrangements in somatic hybrids between tetraploid Medicago sativa and diploid Medicago falcata (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 1112-1118 
    Details
    ISSN: 1432-2242
    Keywords: Key words Somatic hybridization ; rDNA locus ; Chromosome rearrangements ; Gene transfer ; Forage legumes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The aim of this study was to produce somatic hybrids between tetraploid (2n=4x=32) M. sativa and diploid (2n=2x=16) M.  falcata and analyse their genomic structure. Protoplasts from genotypes selected for regeneration ability from the cultivar Rangelander of M. sativa and Wisfal-1 of M. falcata were electrofused. Seven somatic hybrid calli were produced and one of them regenerated plants. The hybrid nature of these plants and their genetic composition were assessed with morphological, cytological, and molecular analyses. The resulting plants were hyper-aneuploid (2n=33) and contained one extra long chromosome, indicating that a translocation had taken place. The presence of both types of parental sequences in the RAPDs analysis confirmed the true hybrid nature of the plants. Rearrangements within the parental genomes and the presence of somaclonal variation among hybrid plants were observed through an RFLP analysis of the nucleolar organizing region (NOR). The possible causes for the gross genomic alterations, and the suitability of this method for transferring useful agronomic traits from wild species to cultivated alfalfa, are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050670
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Plant regeneration from explant and protoplast derived calluses of Medicago littoralis (1995)
    Springer
    Plant cell, tissue and organ culture 41 (1995), S. 41-48 
    Details
    ISSN: 1573-5044
    Keywords: callus ; Medicago littoralis ; plant regeneration ; protoplasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plant regeneration from explant and protoplast derived callus has been achieved in Medicago littoralis cv. Harbinger 1886, an annual legume resistant to the fungus Pseudopeziza medicaginis. Callus was induced from different tissue explants and the fastest growth rate was observed for hypocotyls in B5 medium with 2 mg l−1 2,4-dichlorophenoxyacetic acid and 0.5 mg l−1 N6-benzyladenine. Protoplasts were isolated from cotyledons and leaves of sterile plants and from callus; the first two kinds of protoplasts showed a plating efficiency of 5.6% and 5%, respectively, when embedded in agarose. Plant regeneration occurred on media containing % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9qq-f0-yqaqVeLsFr0-vr% 0-vr0db8meaabaqaciGacaGaaeqabaWaaeaaeaaakeaacaqGobWaaW% baaSqabeaacaqG2aaaaOGaaeOVfiaabs5adaahaaWcbeqaaiaaikda% aaGccaqG+waaaa!3F97!\[{\text{N}}^{\text{6}} {\text{\Delta }}^2 {\text{}}\]isopentenyl-adenine combined with indole-3-acetic acid or 1,2-benzisoxazole-3-acetic acid, and on media with N6-benzyladenine plus α-naphtaleneacetic acid; a cytokinin/auxin ratio higher than 1 induced embryos while a ratio around 1 stimulated shoot formation. Embryo development and rooting of shoots were performed in RL medium without growth regulators.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00124085
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    An efficient and rapid procedure for plantlet regeneration from chicory mesophyll protoplasts (2000)
    Springer
    Plant cell, tissue and organ culture 62 (2000), S. 85-88 
    Details
    ISSN: 1573-5044
    Keywords: Ca-alginate droplets ; chicory ; mesophyll protoplasts ; organogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An efficient procedure for plantlet regeneration from chicory mesophyll protoplasts has been developed in order to perform protoplast fusion experiments. Protoplasts were isolated from a genotype of Italian red chicory (CH 363) and purified by centrifugation in a solution containing 13% (w/v) sucrose to collect uniform protoplasts in size. After 2 days culture at a density of 2×104 protoplasts ml−1 of liquid medium, protoplasts were cultured following three different procedures: in liquid medium, stratified in semi-solid medium, and embedded in Ca-alginate droplets. Four different media were used and culture procedures were evaluated recording the protoplast viability, protoplast division frequency and plating efficiency for each experiment. The embedding of protoplasts in Ca-alginate droplets enhanced both division frequency and plating efficiency for chicory mesophyll cells. Furthermore, this procedure shortened the cycle of plant regeneration from protoplasts, which could be completed in eight weeks.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006477502644
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Plant regeneration and genetic transformation of Lotus angustissimus (1996)
    Springer
    Plant cell, tissue and organ culture 45 (1996), S. 145-152 
    Details
    ISSN: 1573-5044
    Keywords: Agrobacterium rhizogenes ; callus ; shoot organogenesis ; tannins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Culture conditions have been established for callus induction and growth from different explants in L. angustissimus L. Calli were obtained from hypocotyls, leaves, stems, cotyledons and roots cultured on media containing 2,4-dichlorophenoxyacetic acid or α-naphthaleneacetic acid with kinetin, N6 − Δ2 or benzyladenine in different combinations and concentrations. Only those calli induced in presence of α-naphthaleneacetic acid with benzyladenine or kinetin produced shoots. Calli induced from hypocotyl explants were the most efficient in regeneration of shoots. Transformation with an Agrobacterium rhizogenes binary vector carrying the plasmid pBI 121.1 is reported. The percentage of cotransformation was estimated by testing GUS activity in hairy roots. The integration of Ri T-DNA and the NPTII gene in transformed plants was confirmed by molecular analyses and in vitro culture of transgenic tissues in the presence of kanamycin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048758
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    Paper (German National Licenses)
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